LOCUS       sequence-274725-        6352 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses activated Fyn kinase in mec-4 neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6352)
  AUTHORS   Ryan VH, Perdikari TM, Naik MT, Saueressig CF, Lins J, Dignon GL, 
            Mittal J, Hart AC, Fawzi NL
  TITLE     Tyrosine phosphorylation regulates hnRNPA2 granule protein 
            partitioning and reduces neurodegeneration.
  JOURNAL   EMBO J. 2021 Feb 1;40(3):e105001. doi: 10.15252/embj.2020105001. 
            Epub 2020 Dec 22.
  PUBMED    33349959
REFERENCE   2  (bases 1 to 6352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2021-02-1"; volume: "40(3):e105001. doi"; pages: " 
            10.15252/embj.2020105001. Epub 2020 Dec 22"
COMMENT     Sequence Label: pHA852 - mec-4p::FynY531F
FEATURES             Location/Qualifiers
     source          1..6352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     72..91
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(310..414)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      441..896
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(528..547)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     738..759
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1022..1044
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1036..1053
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1037..1053
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1063..1082
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1063..1081
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4601..4621)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4601..4619)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4640..4656)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4640..4656)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4653..4675)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4664..4680
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4688..4694,4695..4712,4713..4718))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4733..4754
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4871..4888)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5042..5630)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5122..5141)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5801..6352,1..240,241..309))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
       61 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
      121 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
      181 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
      241 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
      301 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
      361 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
      421 tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
      481 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
      541 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
      601 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
      661 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
      721 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
      781 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
      841 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttccat
      901 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
      961 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     1021 tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag
     1081 ggcgaattgg agctccaccg cggtggcggc cgctctagaa ctagtaagct tcaatacaag
     1141 ctcaaataca atactatttt ttttccgtct gaacataaaa ttagtttcta aataaaaaat
     1201 gtgaaaataa atatttggtt ttaatcgtta aaatcttcca aattactaat aaagttcagt
     1261 caactatatg cttcgaaatt cactgaatct attattgtta ttaattttta gacccttgga
     1321 gagctcccaa ctctttcaat ccagaagact gatggatgcc aggtgtactt gtcaaaggtg
     1381 gctcaaggat gcgagattgt cacttccaaa tccagtgaga tgaacatctc cgtgcaaacc
     1441 aatgatgatg gggattatgt aagtttttcg gattgtagaa cccaatctaa atttcctttt
     1501 cagtcggagt tcccggttcc ggagcagttc aagacaactt ttgtcaatgg caagctagtc
     1561 actgtcgtct cggacatttg ctaaatcttc tcacgtcata accttatttt tcaacttcat
     1621 gaactatccc ctcgaacttt tttatttgtc cctgtctaca attctttata agcaattgag
     1681 acacactttg ctttattaaa taataccgat tttgttttgt atatgccttg actggtgctc
     1741 aactattttt attgaaaaaa ttacttttta ctgtctcgtc gaataaaagt gatcccttcc
     1801 aaaaaatagt tattcatgtt tgaaggtttg tgatcgtaga acaattgcac cgaatgtagt
     1861 gtaaaaaaaa ttccagaatg ttcaaataca tttccaccct caccagaaca tctcaaacac
     1921 caggcacatt tcaacacact ttcatggatc ttatcttgcc tcaaaaccat cagctttctt
     1981 ctcattctac aagtatgaaa caatctacta ttctatacat ttataatgta atggaaccat
     2041 gtgaagacat gagcaagatc attgtcgtca gcgactaccc ctccttcatt tttcttagag
     2101 tatcagtttg ctaatgctat tttttttatc gctatcaagt tatagaggga tccccgggat
     2161 tggccaaagg acccaaaggt atgtttcgaa tgatactaac ataacataga acattttcag
     2221 gaggaccctt gcttggaggg taccggtaga aaaaatgggc tgtgtgcaat gtaaggataa
     2281 agaagcaaca aaactgacgg aggagaggga cggcagcctg aaccagagct ctgggtaccg
     2341 ctatggcaca gaccccaccc ctcagcacta ccccagcttc ggtgtgacct ccatccccaa
     2401 ctacaacaac ttccacgcag ccgggggcca aggactcacc gtctttggag gtgtgaactc
     2461 ttcgtctcat acggggacct tgcgtacgag aggaggaaca ggagtgacac tctttgtggc
     2521 cctttatgac tatgaagcac ggacagaaga tgacctgagt tttcacaaag gagaaaaatt
     2581 tcaaatattg aacagctcgg aaggagattg gtgggaagcc cgctccttga caactggaga
     2641 gacaggttac attcccagca attatgtggc tccagttgac tctatccagg cagaagagtg
     2701 gtactttgga aaacttggcc gaaaagatgc tgagcgacag ctattgtcct ttggaaaccc
     2761 aagaggtacc tttcttatcc gcgagagtga aaccaccaaa ggtgcctatt cactttctat
     2821 ccgtgattgg gatgatatga aaggagacca tgtcaaacat tataaaattc gcaaacttga
     2881 caatggtgga tactacatta ccacccgggc ccagtttgaa acacttcagc agcttgtaca
     2941 acattactca gagagagctg caggtctctg ctgccgccta gtagttccct gtcacaaagg
     3001 gatgccaagg cttaccgatc tgtctgtcaa aaccaaagat gtctgggaaa tccctcgaga
     3061 atccctgcag ttgatcaaga gactgggaaa tgggcagttt ggggaagtat ggatgggtac
     3121 ctggaatgga aacacaaaag tagccataaa gactcttaaa ccaggcacaa tgtcccccga
     3181 atcattcctt gaggaagcgc agatcatgaa gaagctgaag cacgacaagc tggtccagct
     3241 ctatgcagtg gtgtctgagg agcccatcta catcgtcacc gagtatatga acaaaggaag
     3301 tttactggat ttcttaaaag atggagaagg aagagctctg aaattaccaa atcttgtgga
     3361 catggcagca caggtggctg caggaatggc ttacatcgag cgcatgaatt atatccatag
     3421 agatctgcga tcagcaaaca ttctagtggg gaatggactc atatgcaaga ttgctgactt
     3481 cggattggcc cgattgatag aagacaatga gtacacagca agacaaggtg caaagttccc
     3541 catcaagtgg acggcccccg aggcagccct gtacgggagg ttcacaatca agtctgacgt
     3601 gtggtctttt ggaatcttac tcacagagct ggtcaccaaa ggaagagtgc catacccagg
     3661 catgaacaac cgggaggtgc tggagcaggt ggagcgaggc tacaggatgc cctgcccgca
     3721 ggactgcccc atctctctgc atgagctcat gatccactgc tggaaaaagg accctgaaga
     3781 acgccccact tttgagtact tgcagagctt cctggaagac tactttaccg cgacagagcc
     3841 ccagttccaa cctggtgaaa acctgtaagt ccaattactc ttcaacatcc ctacatgctc
     3901 tttctccctg tgctcccacc ccctattttt gttattatca aaaaacttct cttaatttct
     3961 ttgtttttta gcttctttta agtcacctct aacaatgaaa ttgtgtagat tcaaaaatag
     4021 aattaattcg taataaaaag tcgaaaaaaa ttgtgctccc tccccccatt aataataatt
     4081 ctatcccaaa atctacacaa tgttctgtgt acacttctta tgttttttac ttctgataaa
     4141 tttttttgaa acatcataga aaaaaccgca cacaaaatac cttatcatat gttacgtttc
     4201 agtttatgac cgcaattttt atttcttcgc acgtctgggc ctctcatgac gtcaaatcat
     4261 gctcatcgtg aaaaagtttt ggagtatttt tggaattttt caatcaagtg aaagtttatg
     4321 aaattaattt tcctgctttt gctttttggg gtttccccta ttgtttgtca agatttcgag
     4381 gacggcgttt ttcttgctaa aatcacaagt attgatgagc acgatgcaag aaagatcgga
     4441 agaaggtttg ggtttgaggc tcagtggaag gtgagtagaa gttgataatt tgaaagtgga
     4501 gtagtgtcta tggggttttt gccttaaatg acagaataca ttcccaatat accaaacata
     4561 actgtttctc gagggggggc ccggtaccca gcttttgttc cctttagtga gggttaattg
     4621 cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     4681 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     4741 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     4801 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     4861 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     4921 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     4981 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     5041 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     5101 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     5161 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     5221 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     5281 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     5341 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     5401 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     5461 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     5521 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     5581 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     5641 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     5701 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     5761 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     5821 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     5881 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     5941 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     6001 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     6061 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     6121 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     6181 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     6241 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     6301 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt ga
//
