LOCUS       sequence-274729-        5482 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Control vector for Fyn kinase expression in mec-4 neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5482)
  AUTHORS   Ryan VH, Perdikari TM, Naik MT, Saueressig CF, Lins J, Dignon GL, 
            Mittal J, Hart AC, Fawzi NL
  TITLE     Tyrosine phosphorylation regulates hnRNPA2 granule protein 
            partitioning and reduces neurodegeneration.
  JOURNAL   EMBO J. 2021 Feb 1;40(3):e105001. doi: 10.15252/embj.2020105001. 
            Epub 2020 Dec 22.
  PUBMED    33349959
REFERENCE   2  (bases 1 to 5482)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2021-02-1"; volume: "40(3):e105001. doi"; pages: " 
            10.15252/embj.2020105001. Epub 2020 Dec 22"
COMMENT     Sequence Label: pHA853 - mec-4p::Fynempty
FEATURES             Location/Qualifiers
     source          1..5482
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     116..138
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     130..147
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     131..147
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     157..176
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        157..175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2825..2845)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2825..2843)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2864..2880)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2864..2880)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2877..2899)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2888..2904
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2912..2918,2919..2936,2937..2942))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2957..2978
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3095..3112)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3266..3854)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3346..3365)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4025..4816,4817..4885))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4648..4667
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4886..4990)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5017..5472
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5104..5123)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5314..5335
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
       61 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
      121 tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa
      181 ttggagctcc accgcggtgg cggccgctct agaactagta agcttcaata caagctcaaa
      241 tacaatacta ttttttttcc gtctgaacat aaaattagtt tctaaataaa aaatgtgaaa
      301 ataaatattt ggttttaatc gttaaaatct tccaaattac taataaagtt cagtcaacta
      361 tatgcttcga aattcactga atctattatt gttattaatt tttagaccct tggagagctc
      421 ccaactcttt caatccagaa gactgatgga tgccaggtgt acttgtcaaa ggtggctcaa
      481 ggatgcgaga ttgtcacttc caaatccagt gagatgaaca tctccgtgca aaccaatgat
      541 gatggggatt atgtaagttt ttcggattgt agaacccaat ctaaatttcc ttttcagtcg
      601 gagttcccgg ttccggagca gttcaagaca acttttgtca atggcaagct agtcactgtc
      661 gtctcggaca tttgctaaat cttctcacgt cataacctta tttttcaact tcatgaacta
      721 tcccctcgaa cttttttatt tgtccctgtc tacaattctt tataagcaat tgagacacac
      781 tttgctttat taaataatac cgattttgtt ttgtatatgc cttgactggt gctcaactat
      841 ttttattgaa aaaattactt tttactgtct cgtcgaataa aagtgatccc ttccaaaaaa
      901 tagttattca tgtttgaagg tttgtgatcg tagaacaatt gcaccgaatg tagtgtaaaa
      961 aaaattccag aatgttcaaa tacatttcca ccctcaccag aacatctcaa acaccaggca
     1021 catttcaaca cactttcatg gatcttatct tgcctcaaaa ccatcagctt tcttctcatt
     1081 ctacaagtat gaaacaatct actattctat acatttataa tgtaatggaa ccatgtgaag
     1141 acatgagcaa gatcattgtc gtcagcgact acccctcctt catttttctt agagtatcag
     1201 tttgctaatg ctattttttt tatcgctatc aagttataga gggatccccg ggattggcca
     1261 aaggacccaa aggtatgttt cgaatgatac taacataaca tagaacattt tcaggaggac
     1321 ccttgcttgg agggtaccgg tagaaaaaat gggctgtgtg caatgtaagg ataaagaagc
     1381 aacaaaactg acggaggaga gggacggcag cctgaaccag agctctgggt accgctatgg
     1441 cacagacccc acccctcagc actaccccag cttcggtgtg acctccatcc ccaactacaa
     1501 caacttccac gcagccgggg gccaaggact caccgtcttt ggaggtgtga actcttcgtc
     1561 tcatacgggg accttgcgta cgagaggagg aacaggagtg acactctttg tggcccttta
     1621 tgactatgaa gcacggacag aagatgacct gagttttcac aaaggagaaa aatttcaaat
     1681 attgaacagc tcggaaggag attggtggga agcccgctcc ttgacaactg gagagacagg
     1741 ttacattccc agcaattatg tggctccagt ttggacggcc cccgaggcag ccctgtacgg
     1801 gaggttcaca atcaagtctg acgtgtggtc ttttggaatc ttactcacag agctggtcac
     1861 caaaggaaga gtgccatacc caggcatgaa caaccgggag gtgctggagc aggtggagcg
     1921 aggctacagg atgccctgcc cgcaggactg ccccatctct ctgcatgagc tcatgatcca
     1981 ctgctggaaa aaggaccctg aagaacgccc cacttttgag tacttgcaga gcttcctgga
     2041 agactacttt accgcgacag agccccagtt ccaacctggt gaaaacctgt aagtccaatt
     2101 actcttcaac atccctacat gctctttctc cctgtgctcc caccccctat ttttgttatt
     2161 atcaaaaaac ttctcttaat ttctttgttt tttagcttct tttaagtcac ctctaacaat
     2221 gaaattgtgt agattcaaaa atagaattaa ttcgtaataa aaagtcgaaa aaaattgtgc
     2281 tccctccccc cattaataat aattctatcc caaaatctac acaatgttct gtgtacactt
     2341 cttatgtttt ttacttctga taaatttttt tgaaacatca tagaaaaaac cgcacacaaa
     2401 ataccttatc atatgttacg tttcagttta tgaccgcaat ttttatttct tcgcacgtct
     2461 gggcctctca tgacgtcaaa tcatgctcat cgtgaaaaag ttttggagta tttttggaat
     2521 ttttcaatca agtgaaagtt tatgaaatta attttcctgc ttttgctttt tggggtttcc
     2581 cctattgttt gtcaagattt cgaggacggc gtttttcttg ctaaaatcac aagtattgat
     2641 gagcacgatg caagaaagat cggaagaagg tttgggtttg aggctcagtg gaaggtgagt
     2701 agaagttgat aatttgaaag tggagtagtg tctatggggt ttttgcctta aatgacagaa
     2761 tacattccca atataccaaa cataactgtt tctcgagggg gggcccggta cccagctttt
     2821 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg
     2881 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     2941 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     3001 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     3061 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     3121 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     3181 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     3241 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     3301 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     3361 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     3421 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     3481 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     3541 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     3601 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     3661 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     3721 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     3781 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     3841 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     3901 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     3961 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     4021 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     4081 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     4141 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     4201 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     4261 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     4321 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     4381 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     4441 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     4501 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     4561 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     4621 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     4681 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     4741 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     4801 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     4861 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     4921 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     4981 gggttccgcg cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa
     5041 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     5101 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     5161 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     5221 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     5281 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     5341 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     5401 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     5461 cgcttacaat ttccattcgc ca
//
