LOCUS       sequence-27473-e         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Muona M, Aranko AS, Iwai H
  TITLE     Segmental isotopic labelling of a multidomain protein by protein 
            ligation by protein trans-splicing.
  JOURNAL   Chembiochem. 2008 Dec 15. 9(18):2958-61.
  PUBMED    19031436
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Chembiochem. 2008 Dec 15. 9(18):2958-61."
COMMENT     Sequence Label: pMMRSF1-16
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..92
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             460..504
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(542..560)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      556..603
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aataattttg tttaacttta ataaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcag cggcctggtg ccgcgcggca gccatatgga ccataatttt gcactcaaaa
      121 atggcttcat agcttctaat tgtttcaatg gtaccctggg tgggccggag cctgggccct
      181 atgcccaacc cagcgtcaac actccgctcc ctaacctcca gaatgggccc atttatgcca
      241 gggttatcca gaagcgagtc cctaatgcct acgacaagac agccttggct ttggaggtcg
      301 gtgagctggt aaaggttacg aagattaatg tgagtggtca gtgggaaggg gagtgtaatg
      361 gcaaacgagg tcacttccca ttcacacatg tccgtctgct ggatcaacag aatcccgatg
      421 aggacttcag ctaagcttgc ggccgcactc gagtctggta aagaaaccgc tgctgcgaaa
      481 tttgaacgcc agcacatgga ctcgtctact agcgcagctt aattaaccta ggctgctgcc
      541 accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
      601 ttgctgaaac ctcaggcatt tgagaagcac acggtcacac tgcttccggt agtcaataaa
      661 ccggtaaacc agcaatagac ataagcggct atttaacgac cctgccctga accgacgaca
      721 agctgacgac cgggtctccg caagtggcac ttttcgggga aatgtgcgcg a
//
