LOCUS       sequence-27474-e         619 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Muona M, Aranko AS, Iwai H
  TITLE     Segmental isotopic labelling of a multidomain protein by protein 
            ligation by protein trans-splicing.
  JOURNAL   Chembiochem. 2008 Dec 15. 9(18):2958-61.
  PUBMED    19031436
REFERENCE   2  (bases 1 to 619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Chembiochem. 2008 Dec 15. 9(18):2958-61."
COMMENT     Sequence Label: pMMRSF17
FEATURES             Location/Qualifiers
     source          1..619
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             72..362
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
ORIGIN
        1 aataattttg tttaacttta ataaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcac tagtgactca gaagtcaatc aagaagctaa gccagaggtc aagccagaag
      121 tcaagcctga gactcacatc aatttaaagg tgtccgatgg atcttcagaa atcttcttta
      181 agatcaaaaa gaccactcct ttaagaaggc tgatggaagc gttcgctaaa agacagggta
      241 aggaaatgga ctccttaaga ttcttgtacg acggtattag aattcaagct gatcagaccc
      301 ctgaagattt ggacatggag gataacgata ttattgaggc tcacagagaa cagattggtg
      361 gttcgaagag ccatatgcag gaggaggcag agtatgtgcg ggccctcttt gactttaatg
      421 ggaatgatga agaagatctt ccctttaaga aaggagacat cctgagaatc cgggataagc
      481 ctgaagagca gtggtggaat gcagaggaca gcgaaggaaa gagggggatg attcctgtcc
      541 cttacgtgga gaagtataga cctgcctccg cctcagtatc ggctctgatt ggaggtaacc
      601 agggatcctg tttaagcta
//
