LOCUS       sequence-274764-       13377 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used to generate a transgene that will express an epitope tagged 
            Chitin Synthase in Drosophila using Gal4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13377)
  AUTHORS   Adler PN
  TITLE     The localization of chitin synthase mediates the patterned 
            deposition of chitin in developing Drosophila bristles
  JOURNAL   bioRxiv 2020
REFERENCE   2  (bases 1 to 13377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2020"
COMMENT     Sequence Label: pUAS-kkv-OH
FEATURES             Location/Qualifiers
     source          1..13377
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    333..366
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    399..493
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        512..750
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     697..718
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             complement(1477..1488)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      2772..2781
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5643..5684
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             5685..5702
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     intron          5792..5857
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5987..6007
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6279..6413
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6316..6335)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6370..6389
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    6442..6511
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(6730..6748)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6769..6785)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6769..6785)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6782..6804)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6793..6809
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6817..6823,6824..6841,6842..6847))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6862..6883
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7000..7017)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7171..7759)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7251..7270)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7930..8721,8722..8790))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8553..8572
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8791..8895)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8921..9376)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9058..9079)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9270..9289
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9503..9525
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9517..9534
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9518..9534
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9541..9560
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9541..9559
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9593..9611)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(10063..10134,10536..10809,10884..11538,11600..11915,
                     12119..12250,12321..12935)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
ORIGIN
        1 gagtggttca tttcgtctca atagaaatta gtaataaata tttgtatgta caatttattt
       61 gctccaatat atttgtatat atttccctca cagctatatt tattctaatt taatattatg
      121 actttttaag gtaatttttt gtgacctgtt cggagtgatt agcgttacaa tttgaactga
      181 aagtgacatc cagtgtttgt tccttgtgta gatgcatctc aaaaaaatgg tgggcataat
      241 agtgttgttt atatatatca aaaataacaa ctataataat aagaatacat ttaatttaga
      301 aaatgcttgg atttcactgg aactaggcta gcataacttc gtataatgta tgctatacga
      361 agttatgcta gcggatccaa gcttgcatgc ctgcaggtcg gagtactgtc ctccgagcgg
      421 agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt
      481 actgtcctcc gagcggagac tctagcgagc gccggagtat aaatagaggc gcttcgtcta
      541 cggagcgaca attcaattca aacaagcaaa gtgaacacgt cgctaagcga aagctaagca
      601 aataaacaag cgcagctgaa caagctaaac aatctgcagt aaagtgcaag ttaaagtgaa
      661 tcaattaaaa gtaaccagca accaagtaaa tcaactgcaa ctactgaaat ctgccaagaa
      721 gtaattattg aatacaagaa gagaactctg aatagggaat tgggaattcg ttaacagatc
      781 tgcggccgcg gctcgagatg tctgcgatgc ggcatcgccc gatggcccct ccgggccaag
      841 gaccaggagc gggaaccgct ggggagcacg tcgacagcga tgacaacaac tttaccgacg
      901 acgagagctc gcccctcacc cacgacattt acggcggcag ccaacgcacc atacaagaga
      961 cgaagggttg ggatgttttc cgggatccgc caatcaaaat tgagaccgga tcgacggcga
     1021 accaggaatg cttagaatta accgtgaaga tcttgaagat cttcgcctat gtgattacgt
     1081 ttataatagt tttaaccggt ggcgtcatcg ccaagggcac aatgctcttc atgacttcgc
     1141 aggtgcgcaa ggataagaag atggagtact gcaacaaaga cttgggtcgg gacaaaagct
     1201 tcgtagtccg acttccggag gaggagcgag tggcctggat ctgggctctg ctcatagcat
     1261 atgcccttcc cgagatcgga gccttgatcc gatccgcccg catctgcttc ttcaagacct
     1321 ttaaggtgcc gaagacgggc cacttcctct tcgtctggct gatggagagc ttgagtgccg
     1381 tgggcatggc gctgctgatg ttcgtggttc tgccccagat cgacgccatc cagggcgcca
     1441 tgctaaccaa ctgcctgtgc gtcgtgccgg gcatcttcgg ccttctatcg cgcacctcca
     1501 aggagggcaa gcgctttgta aaggtgatca tcgatctggc ggcggtggcg gcccaggtga
     1561 cgggcctggt gatctggccg ctgctggaga accgccgtga gctgtgggtc atcccggtgg
     1621 cctgtgtcat gatctcgtgc ggctggtggg agaattacgt gtcgccacag tccccgctcg
     1681 gcctggttcg agccctgggt cgcatcaagg aggagatgaa gtacactcgc tacttctgcc
     1741 acatattcct atccatctgg aagatcctgc ttttcttcac cgtcacactg ctcatctact
     1801 gggcgcaagg cgaggaaccc ggcaatctgt ttgccatgta cggagatgcc tttggacccc
     1861 acaagatcat agtctacgaa ctgccagcgg gactgggcgg tgtgcttcca gataccctgg
     1921 aatcggccaa cattgacacc gtggatgtgg atgccgccta caatacggtg gtgtatgtac
     1981 tgcttctaca gattttcggc gcctacttgt gctacatctt cggaaagttc gcctgcaaga
     2041 tcctcatcca gggattcagc tacgcgtttc ccgtcagtct aactgttccg ctctctgtta
     2101 cgttcctgat cgcagcctgt ggaatccgga tcgacgatcc ctgcttcttc catgacacca
     2161 ttccggacta tctgttcttc acgagcccct cgaacttccg gttcaacaac tttgtcaccg
     2221 agcaaatggc gtgggcctgg atcctttggc tcctcagtca gacctggata gcactgcaca
     2281 tctggactcc gaagtgcgag cgtctggcca ccaccgagaa gttgtttgtc cagcccatgt
     2341 actcctctct actgatcgat cagtcgatgg ccctcaaccg aaggcgcgat gaccaggcag
     2401 atgtgaaaac agaggatctc tcggagatcg aaaaggaaaa gggcgatgag tactacgaga
     2461 ccatttctgt gcacacggat cgctcctcgg cacccaacaa gccatcgatt aagtcctccg
     2521 acaacatcac acgaatctac tcctgcgcga ccatgtggca tgagacgaag gacgagatga
     2581 tagagttctt gaagagcatc atgcgaatgg acgaggacca gtgtgctcgt cgcgtggctc
     2641 aaaagtatct gagggttctc gatccggact attatgaatt tgagacccac atcttcttcg
     2701 atgacgcctt cgaaatctcc gatcacagcg acgatgatat ccagtgcaat cgattcgtca
     2761 agctcttgat agccaccatg gacgaggctg cctccgagat ccatcagacc acgatcagac
     2821 tgcgtccgcc caagaagtat cccactccgt acggaggccg cctggtgtgg acccttccag
     2881 ggaagaccaa attcattacg cacttaaagg acaaggaccg cattcgtcac aggaagcgtt
     2941 ggtctcaggt catgtacatg tattacctgc tgggacatcg tctaatggag ctgcccattt
     3001 cggtggatcg caaggatgca attgcggaga acacctacct tttgaccctg gacggagata
     3061 ttgacttcaa gccgaatgcg gtgactctac tggtggatct gatgaagaag aacaagaacc
     3121 tgggtgctgc ctgcggtcgc attcatcccg tgggatcggg acccatggtg tggtaccaac
     3181 ttttcgaata cgccatcggc cattggctgc aaaaggccac ggagcacatg attggctgcg
     3241 tgctctgttc acctggatgc ttctccctgt tcaggggcaa ggccctcatg gatgacaatg
     3301 tgatgaagaa atacacgaca cggtcggatg aggctcgtca ctacgtgcag tacgatcagg
     3361 gcgaggatcg ttggctgtgc acattgctcc tccagagggg ataccgcgtg gagtactcgg
     3421 ctgccagtga tgcgtacacc cactgccccg agggattcaa tgagttctac aaccagcggc
     3481 gcagatgggt gccctcgacc atagccaaca tcatggatct tctggcggat gcgaagcgca
     3541 cgatcaagat caatgacaac atttcgcttc tgtacatctt ctaccagatg atgttgatgg
     3601 gcggcaccat cctgggaccc ggaaccattt tccttatgtt ggtgggtgca ttcgtggcag
     3661 cctttcgcat tgacaactgg acctccttcc actacaacat cgtgccgatc ctggccttca
     3721 tgtttatctg cttcacctgt aagtcgaaca tccagttgtt cgtggcccaa gtcctttcga
     3781 cggcatatgc cctgatcatg atggcggtga ttgtgggtac ggccttgcag ttgggagagg
     3841 acggaatagg ctccccttcg gccattttcc tgatatccat ggtgggctca ttcttcatag
     3901 ccgcatgttt gcatccacaa gagttctggt gcattacatg cggcctaatc tatttgctgt
     3961 ccatcccgtc catgtacctg ctgctcatct tgtactctat catcaaccta aacgtcgtct
     4021 cctggggcac ccgcgaggtg gtggctaaga agaccaagaa agaactggag gcggagaaga
     4081 aggccgccga ggaggccaaa aagagggtga agcagaagag catgctgagc ttccttcaga
     4141 gtggaatcgg ggacaatggc gacgaggagg gctccgtgga gttctctctc gctggactct
     4201 tccgctgcat attctgcacc cacggaaaaa catccgacga gaagcagcag ctgacctcca
     4261 tcgccgaatc gctggacacg atcaaacatc gaatggacac catcgagagt gctgtggatc
     4321 cccatggtca ccatgcctcc cgacatggca ggaggagaac cacatccagt ggctccaagg
     4381 atcaccacct gctgacctcg gtggcggaga agtctggcga tgaatcggac gagtcggact
     4441 cagacacttc ggcggaacca aagcaggagc gagactttct aaccaatccc tactggatcg
     4501 aggaccccga tgtacgcaag ggcgaggtcg attttctctc cagcaccgag atccagttct
     4561 ggaaggatct catcgaccag tatctctatc ccatcgacaa tgatcccgtg gagcaggccc
     4621 gcatagccaa agatctgaag gagctgcgcg actcgtcggt gttcgcgttc ttcatgatca
     4681 atgcactgtt cgtgctgatc gtgttcctgc tgcagctgaa caaggacaac atccacgtga
     4741 agtggccctt cggagtgcgg acgaacatca cctacgacga gtccacccag gaggtgcaca
     4801 tctccaagga gtaccttcag ctggagccca tcggattggt gttcgtattc ttctttgctc
     4861 ttattttgat aatccagttc acggccatgc tgttccatcg atttggaacc atttcgcaca
     4921 tcctggcctc gactgaactc aacttctgca agaagaagtc tgaggatctg acacaggacc
     4981 aactaataga taagcatgct gtggagatcg tcaagaactt gcagaggctg cagggaatcg
     5041 atggcgacta cgacaatgac tccggttccg ggccagatcg catagccagg cggaagacca
     5101 ttcaaaatct ggagaaggcg cgacagccac gtcgccagat cggcaccctg gatgtggcgt
     5161 tcaagaagcg cttcctgaaa ctcaccgccg atgcggagaa caatccggcc acgcccattc
     5221 tcacccgccg cctgacaatg cgggcggaga ccatccgggc actggaggtg cgtaagaatt
     5281 cggtgatggc cgaaaggcgg aagtccgcga tgcaaactct gggagcaaag aacgagtacg
     5341 gaatcacaac tggagcaccg attaacaaca atggagctct gccgaatcag cgaagcggac
     5401 gtgtctcgaa tgcaggcatc agcatcaagg atgtattcaa tgtgaacggt ggtggtgccg
     5461 agcagattta cggctcgaat gggggcggca ctatcaacca gggttacgag catgtgatcg
     5521 acgaggacgg cgacggcaac tccctgcggc tgaccaccag gaatccccat ccccacccgc
     5581 accaccaggt ctcctggggc cagaacacca acggcggcgg cggtaatggc acaggtcgcc
     5641 tgtccggctt cgccaacgag ctgggccccc gcctgatggg caagcatcac catcatcacc
     5701 actaatagtc tagaggatct ttgtgaagga accttacttc tgtggtgtga cataattgga
     5761 caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg
     5821 tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga
     5881 atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc
     5941 atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag
     6001 aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt
     6061 gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact
     6121 gctatacaag aaaattatgg aaaaatattt gatgtatagt gccttgacta gagatcataa
     6181 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     6241 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     6301 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     6361 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccactagt
     6421 gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg tgccagggcg tgcccttggg
     6481 ctccccgggc gcgtactcca cctcacccat ctggtccatc atgatgaacg ggtcgaggtg
     6541 gcggtagttg atcccggcga acgcgcggcg caccgggaag ccctcgccct cgaaaccgct
     6601 gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg tcggcgggtg cggatacgcg
     6661 gggcagcgtc agcgggttct cgacggtcac ggcgggcatg tcgacactag ttctagccag
     6721 cttttgttcc ctttagtgag ggttaatttc gagcttggcg taatcatggt catagctgtt
     6781 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     6841 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     6901 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     6961 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     7021 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     7081 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     7141 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     7201 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     7261 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     7321 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     7381 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     7441 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     7501 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     7561 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     7621 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     7681 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     7741 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     7801 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     7861 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     7921 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     7981 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     8041 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     8101 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     8161 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     8221 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     8281 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     8341 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     8401 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     8461 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     8521 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     8581 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     8641 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     8701 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     8761 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     8821 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     8881 aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt
     8941 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     9001 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     9061 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     9121 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     9181 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     9241 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     9301 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     9361 ccgctacagg gcgcgtccca ttcgccattc aggctgcgca actgttggga agggcgatcg
     9421 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     9481 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattg
     9541 taatacgact cactataggg cgaattgggt acgtaccggg cccctagtat gtatgtaagt
     9601 taataaaacc catttttgcg gaaagtagat aaaaaaaaca tttttttttt ttactgcact
     9661 ggatatcatt gaacttatct gatcagtttt aaatttactt cgatccaagg gtatttgatg
     9721 taccaggttc tttcgattac ctctcactca aaatgacatt ccactcaaag tcagcgctgt
     9781 ttgcctcctt ctctgtccac agaaatatcg ccgtctcttt cgccgctgcg tccgctatct
     9841 ctttcgccac cgtttgtagc gttacgtagc gtcaatgtcc gccttcagtt gcattttgtc
     9901 agcggtttcg tgacgaagct ccaagcggtt tacgccatca attaaacaca aagtgctgtg
     9961 ccaaaactcc tctcgcttct tatttttgtt tgttttttga gtgattgggg tggtgattgg
    10021 ttttgggtgg gtaagcaggg gaaagtgtga aaaatcccgg caatgggcca agaggatcag
    10081 gagctattaa ttcgcggagg cagcaaacac ccatctgccg agcatctgaa caatgtgagt
    10141 agtacatgtg catacatctt aagttcactt gatctatagg aactgcgatt gcaacatcaa
    10201 attgtctgcg gcgtgagaac tgcgacccac aaaaatccca aaccgcaatt gcacaaacaa
    10261 atagtgacac gaaacagatt attctggtag ctgttctcgc tatataagac aatttttgag
    10321 atcatatcat gatcaagaca tctaaaggca ttcattttcg actatattct tttttacaaa
    10381 aaatataaca accagatatt ttaagctgat cctagatgca caaaaaataa ataaaagtat
    10441 aaacctactt cgtaggatac ttcggggtac tttttgttcg gggttagatg agcataacgc
    10501 ttgtagttga tatttgagat cccctatcat tgcagggtga cagcggagcg gcttcgcaga
    10561 gctgcattaa ccagggcttc gggcaggcca aaaactacgg cacgctccgg ccacccagtc
    10621 cgccggagga ctccggttca gggagcggcc aactagccga gaacctcacc tatgcctggc
    10681 acaatatgga catctttggg gcggtcaatc agccgggctc cggatggcgg cagctggtca
    10741 accggacacg cggactattc tgcaacgagc gacacatacc ggcgcccagg aaacatttgc
    10801 tcaagaacgg tgagtttcta ttcgcagtcg gctgatctgt gtgaaatctt aataaagggt
    10861 ccaattacca atttgaaact cagtttgcgg cgtggcctat ccgggcgaac ttttggccgt
    10921 gatgggcagt tccggtgccg gaaagacgac cctgctgaat gcccttgcct ttcgatcgcc
    10981 gcagggcatc caagtatcgc catccgggat gcgactgctc aatggccaac ctgtggacgc
    11041 caaggagatg caggccaggt gcgcctatgt ccagcaggat gacctcttta tcggctccct
    11101 aacggccagg gaacacctga ttttccaagc catggtgcgg atgccacgac atctgaccta
    11161 tcggcagcga gtggcccgcg tggatcaggt gatccaggag ctttcgctca gcaaatgtca
    11221 gcacacgatc atcggtgtgc ccggcagggt gaaaggtctg tccggcggag aaaggaagcg
    11281 tctggcattc gcctccgagg ctctaaccga tccgccgctt ctgatctgcg atgagcccac
    11341 ctccggactg gactccttta ccgcccacag cgtcgtccag gtgctgaaga agctgtcgca
    11401 gaagggcaag accgtcatcc tgaccattca tcagccgtct tccgagctgt ttgagctctt
    11461 tgacaagatc cttctgatgg ccgagggcag ggtagctttc ttgggcactc ccagcgaagc
    11521 cgtcgacttc ttttcctagt gagttcgatg tgtttattaa gggtatctag tattacataa
    11581 catctcaact cctatccagc gtgggtgccc agtgtcctac caactacaat ccggcggact
    11641 tttacgtaca ggtgttggcc gttgtgcccg gacgggagat cgagtcccgt gatcggatcg
    11701 ccaagatatg cgacaatttt gccattagca aagtagcccg ggatatggag cagttgttgg
    11761 ccaccaaaaa tctggagaag ccactggagc agccggagaa tgggtacacc tacaaggcca
    11821 cctggttcat gcagttccgg gcggtcctgt ggcgatcctg gctgtcggtg ctcaaggaac
    11881 cactcctcgt aaaagtgcga cttattcaga caacggtgag tggttccagt ggaaacaaat
    11941 gatataacgc ttacaattct tggaaacaaa ttcgctagat tttagataga attgcctgat
    12001 tccacaccct tcttagtttt tttcaatgag atgtatagtt tatagttttg cagaagataa
    12061 ataaatttca tttaactcgc gaatattaat gagatgcgag taacatttta atttgcagat
    12121 ggttgccatc ttgattggcc tcatcttttt gggccaacaa ctcacgcaag tgggtgtgat
    12181 gaatatcaac ggagccatct tcctcttcct gaccaacatg acctttcaaa acgtctttgc
    12241 cacgataaat gtaagtcatg tttagaatac atttgcattt caataattta ctaactttct
    12301 aatgaatcga ttcgatttag gtgttcacct cagagctgcc agtttttatg agggaggccc
    12361 gaagtcgact ttatcgctgt gacacatact ttctgggcaa aacgattgcc gaattgccgc
    12421 tttttctcac agtgccactg gtcttcacgg cgattgccta tccgatgatc ggactgcggg
    12481 ccggagtgct gcacttcttc aactgcctgg cgctggtcac tctggtggcc aatgtgtcaa
    12541 cgtccttcgg atatctaata tcctgcgcca gctcctcgac ctcgatggcg ctgtctgtgg
    12601 gtccgccggt tatcatacca ttcctgctct ttggcggctt cttcttgaac tcgggctcgg
    12661 tgccagtata cctcaaatgg ttgtcgtacc tctcatggtt ccgttacgcc aacgagggtc
    12721 tgctgattaa ccaatgggcg gacgtggagc cgggcgaaat tagctgcaca tcgtcgaaca
    12781 ccacgtgccc cagttcgggc aaggtcatcc tggagacgct taacttctcc gccgccgatc
    12841 tgccgctgga ctacgtgggt ctggccattc tcatcgtgag cttccgggtg ctcgcatatc
    12901 tggctctaag acttcgggcc cgacgcaagg agtagccgac atatatccga aataactgct
    12961 tgtttttttt ttttaccatt attaccatcg tgtttactgt ttattgcccc ctcaaaaagc
    13021 taatgtaatt atatttgtgc caataaaaac aagatatgac ctatagaata caagtatttc
    13081 cccttcgaac atccccacaa gtagactttg gatttgtctt ctaaccaaaa gacttacaca
    13141 cctgcatacc ttacatcaaa aactcgttta tcgctacata aaacaccggg atatattttt
    13201 tatatacata cttttcaaat cgcgcgccct cttcataatt cacctccacc acaccacgtt
    13261 tcgtagttgc tctttcgctg tctcccaccc gctctccgca acacattcac cttttgttcg
    13321 acgaccttgg agcgactgtc gttagttccg cgcgattcgg ttcgctcaaa tggttcc
//
