LOCUS       sequence-274779-       14025 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used to generate a transgene that will express a fluorescent Chitin 
            Synthase in Drosophila where the active site is converted to the 
            mosquito sequence. Expression by using Gal4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14025)
  AUTHORS   Adler PN
  TITLE     The localization of chitin synthase mediates the patterned 
            deposition of chitin in developing Drosophila bristles
  JOURNAL   bioRxiv 2020
REFERENCE   2  (bases 1 to 14025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2020"
COMMENT     Sequence Label: pUAS-kkv-mos::NG
FEATURES             Location/Qualifiers
     source          1..14025
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    40..73
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    106..200
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        219..457
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     404..425
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             complement(1184..1195)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      2479..2488
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5350..6060
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      5605..5614
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          6147..6212
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6342..6362
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6634..6768
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6671..6690)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6725..6744
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    6797..6866
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(7085..7103)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7124..7140)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7124..7140)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7137..7159)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7148..7164
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7172..7178,7179..7196,7197..7202))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7217..7238
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7355..7372)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7526..8114)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7606..7625)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8285..9076,9077..9145))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8908..8927
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9146..9250)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9276..9731)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9413..9434)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9625..9644
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9858..9880
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9872..9889
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9873..9889
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9896..9915
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9896..9914
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9948..9966)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(10418..10489,10891..11164,11239..11893,11955..12270,
                     12474..12605,12676..13290)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
ORIGIN
        1 atttagaaaa tgcttggatt tcactggaac taggctagca taacttcgta taatgtatgc
       61 tatacgaagt tatgctagcg gatccaagct tgcatgcctg caggtcggag tactgtcctc
      121 cgagcggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga
      181 gcggagtact gtcctccgag cggagactct agcgagcgcc ggagtataaa tagaggcgct
      241 tcgtctacgg agcgacaatt caattcaaac aagcaaagtg aacacgtcgc taagcgaaag
      301 ctaagcaaat aaacaagcgc agctgaacaa gctaaacaat ctgcagtaaa gtgcaagtta
      361 aagtgaatca attaaaagta accagcaacc aagtaaatca actgcaacta ctgaaatctg
      421 ccaagaagta attattgaat acaagaagag aactctgaat agggaattgg gaattcgtta
      481 acagatctgc ggccgcggct cgagatgtct gcgatgcggc atcgcccgat ggcccctccg
      541 ggccaaggac caggagcggg aaccgctggg gagcacgtcg acagcgatga caacaacttt
      601 accgacgacg agagctcgcc cctcacccac gacatttacg gcggcagcca acgcaccata
      661 caagagacga agggttggga tgttttccgg gatccgccaa tcaaaattga gaccggatcg
      721 acggcgaacc aggaatgctt agaattaacc gtgaagatct tgaagatctt cgcctatgtg
      781 attacgttta taatagtttt aaccggtggc gtcatcgcca agggcacaat gctcttcatg
      841 acttcgcagg tgcgcaagga taagaagatg gagtactgca acaaagactt gggtcgggac
      901 aaaagcttcg tagtccgact tccggaggag gagcgagtgg cctggatctg ggctctgctc
      961 atagcatatg cccttcccga gatcggagcc ttgatccgat ccgcccgcat ctgcttcttc
     1021 aagaccttta aggtgccgaa gacgggccac ttcctcttcg tctggctgat ggagagcttg
     1081 agtgccgtgg gcatggcgct gctgatgttc gtggttctgc cccagatcga cgccatccag
     1141 ggcgccatgc taaccaactg cctgtgcgtc gtgccgggca tcttcggcct tctatcgcgc
     1201 acctccaagg agggcaagcg ctttgtaaag gtgatcatcg atctggcggc ggtggcggcc
     1261 caggtgacgg gcctggtgat ctggccgctg ctggagaacc gccgtgagct gtgggtcatc
     1321 ccggtggcct gtgtcatgat ctcgtgcggc tggtgggaga attacgtgtc gccacagtcc
     1381 ccgctcggcc tggttcgagc cctgggtcgc atcaaggagg agatgaagta cactcgctac
     1441 ttctgccaca tattcctatc catctggaag atcctgcttt tcttcaccgt cacactgctc
     1501 atctactggg cgcaaggcga ggaacccggc aatctgtttg ccatgtacgg agatgccttt
     1561 ggaccccaca agatcatagt ctacgaactg ccagcgggac tgggcggtgt gcttccagat
     1621 accctggaat cggccaacat tgacaccgtg gatgtggatg ccgcctacaa tacggtggtg
     1681 tatgtactgc ttctacagat tttcggcgcc tacttgtgct acatcttcgg aaagttcgcc
     1741 tgcaagatcc tcatccaggg attcagctac gcgtttcccg tcagtctaac tgttccgctc
     1801 tctgttacgt tcctgatcgc agcctgtgga atccggatcg acgatccctg cttcttccat
     1861 gacaccattc cggactatct gttcttcacg agcccctcga acttccggtt caacaacttt
     1921 gtcaccgagc aaatggcgtg ggcctggatc ctttggctcc tcagtcagac ctggatagca
     1981 ctgcacatct ggactccgaa gtgcgagcgt ctggccacca ccgagaagtt gtttgtccag
     2041 cccatgtact cctctctact gatcgatcag tcgatggccc tcaaccgaag gcgcgatgac
     2101 caggcagatg tgaaaacaga ggatctctcg gagatcgaaa aggaaaaggg cgatgagtac
     2161 tacgagacca tttctgtgca cacggatcgc tcctcggcac ccaacaagcc atcgattaag
     2221 tcctccgaca acatcacacg aatctactcc tgcgcgacca tgtggcatga gacgaaggac
     2281 gagatgatag agttcttgaa gagcatcatg cgaatggacg aggaccagtg tgctcgtcgc
     2341 gtggctcaaa agtatctgag ggttctcgat ccggactatt atgaatttga gacccacatc
     2401 ttcttcgatg acgccttcga aatctccgat cacagcgacg atgatatcca gtgcaatcga
     2461 ttcgtcaagc tcttgatagc caccatggac gaggctgcct ccgagatcca tcagaccacg
     2521 atcagactgc gtccgcccaa gaagtatccc actccgtacg gaggccgcct ggtgtggacc
     2581 cttccaggga agaccaaatt cattacgcac ttaaaggaca aggaccgcat tcgtcacagg
     2641 aagcgttggt ctcaggtcat gtacatgtat tacctgctgg gacatcgtct aatggagctg
     2701 cccatttcgg tggatcgcaa ggatgtaatg gcggagaaca cctacctttt gaccctggac
     2761 ggagatattg acttcaaccc gagtgcggtg actctactgg tggatctgat gaagaagaac
     2821 aagaacctgg gtgctgcctg cggtcgcatt catcccatcg gatcgggacc catggtgtgg
     2881 taccaaaaat tcgaatacgc catcggccat tggctgcaaa aggccacgga gcacatgatt
     2941 ggctgcgtgc tctgttcacc tggatgcttc tccctgttca ggggcaaggg cctcatggat
     3001 gacaatgtga tgaggaaata cacgacacgg tcggatgagg ctcgtcacta cgtgcagtac
     3061 gatcagggcg aggatcgttg gctgtgcaca ttgctcctcc agaggggata ccgcgtggag
     3121 tactcggctg ccagtgatgc gtacacccac tgccccgagg gattcaatga gttctacaac
     3181 cagcggcgca gatgggtgcc ctcgaccata gccaacatca tggatcttct ggcggatgcg
     3241 aagcgcacga tcaagatcaa tgacaacatt tcgcttctgt acatcttcta ccagatgatg
     3301 ttgatgggcg gcaccatcct gggacccgga accattttcc ttatgttggt gggtgcattc
     3361 gtggcagcct ttcgcattga caactggacc tccttccact acaacatcgt gccgatcctg
     3421 gccttcatgt ttatctgctt cacctgtaag tcgaacatcc agttgttcgt ggcccaagtc
     3481 ctttcgacgg catatgccct gatcatgatg gcggtgattg tgggtacggc cttgcagttg
     3541 ggagaggacg gaataggctc cccttcggcc attttcctga tatccatggt gggctcattc
     3601 ttcatagccg catgtttgca tccacaagag ttctggtgca ttacatgcgg cctaatctat
     3661 ttgctgtcca tcccgtccat gtacctgctg ctcatcttgt actctatcat caacctaaac
     3721 gtcgtctcct ggggcacccg cgaggtggtg gctaagaaga ccaagaaaga actggaggcg
     3781 gagaagaagg ccgccgagga ggccaaaaag agggtgaagc agaagagcat gctgagcttc
     3841 cttcagagtg gaatcgggga caatggcgac gaggagggct ccgtggagtt ctctctcgct
     3901 ggactcttcc gctgcatatt ctgcacccac ggaaaaacat ccgacgagaa gcagcagctg
     3961 acctccatcg ccgaatcgct ggacacgatc aaacatcgaa tggacaccat cgagagtgct
     4021 gtggatcccc atggtcacca tgcctcccga catggcagga ggagaaccac atccagtggc
     4081 tccaaggatc accacctgct gacctcggtg gcggagaagt ctggcgatga atcggacgag
     4141 tcggactcag acacttcggc ggaaccaaag caggagcgag actttctaac caatccctac
     4201 tggatcgagg accccgatgt acgcaagggc gaggtcgatt ttctctccag caccgagatc
     4261 cagttctgga aggatctcat cgaccagtat ctctatccca tcgacaatga tcccgtggag
     4321 caggcccgca tagccaaaga tctgaaggag ctgcgcgact cgtcggtgtt cgcgttcttc
     4381 atgatcaatg cactgttcgt gctgatcgtg ttcctgctgc agctgaacaa ggacaacatc
     4441 cacgtgaagt ggcccttcgg agtgcggacg aacatcacct acgacgagtc cacccaggag
     4501 gtgcacatct ccaaggagta ccttcagctg gagcccatcg gattggtgtt cgtattcttc
     4561 tttgctctta ttttgataat ccagttcacg gccatgctgt tccatcgatt tggaaccatt
     4621 tcgcacatcc tggcctcgac tgaactcaac ttctgcaaga agaagtctga ggatctgaca
     4681 caggaccaac taatagataa gcatgctgtg gagatcgtca agaacttgca gaggctgcag
     4741 ggaatcgatg gcgactacga caatgactcc ggttccgggc cagatcgcat agccaggcgg
     4801 aagaccattc aaaatctgga gaaggcgcga cagccacgtc gccagatcgg caccctggat
     4861 gtggcgttca agaagcgctt cctgaaactc accgccgatg cggagaacaa tccggccacg
     4921 cccattctca cccgccgcct gacaatgcgg gcggagacca tccgggcact ggaggtgcgt
     4981 aagaattcgg tgatggccga aaggcggaag tccgcgatgc aaactctggg agcaaagaac
     5041 gagtacggaa tcacaactgg agcaccgatt aacaacaatg gagctctgcc gaatcagcga
     5101 agcggacgtg tctcgaatgc aggcatcagc atcaaggatg tattcaatgt gaacggtggt
     5161 ggtgccgagc agatttacgg ctcgaatggg ggcggcacta tcaaccaggg ttacgagcat
     5221 gtgatcgacg aggacggcga cggcaactcc ctgcggctga ccaccaggaa tccccatccc
     5281 cacccgcacc accaggtctc ctggggccag aacaccaacg gcggcggcgg taatggcaca
     5341 ggtcgcctga tggtgagcaa gggcgaggag gataacatgg cctctctccc agcgacacat
     5401 gagttacaca tctttggctc catcaacggt gtggactttg acatggtggg tcagggcacc
     5461 ggcaatccaa atgatggtta tgaggagtta aacctgaagt ccaccaaggg tgacctccag
     5521 ttctccccct ggattctggt ccctcatatc gggtatggct tccatcagta cctgccctac
     5581 cctgacggga tgtcgccttt ccaggccgcc atggtagatg gctccggata ccaagtccat
     5641 cgcacaatgc agtttgaaga tggtgcctcc cttactgtta actaccgcta cacctacgag
     5701 ggaagccaca tcaaaggaga ggcccaggtg aaggggactg gtttccctgc tgacggtcct
     5761 gtgatgacca actcgctgac cgctgcggac tggtgcaggt cgaagaagac ttaccccaac
     5821 gacaaaacca tcatcagtac ctttaagtgg agttacacca ctggaaatgg caagcgctac
     5881 cggagcactg cgcggaccac ctacaccttt gccaagccaa tggcggctaa ctatctgaag
     5941 aaccagccga tgtacgtgtt ccgtaagacg gagctcaagc actccaagac cgagctcaac
     6001 ttcaaggagt ggcaaaaggc ctttaccgat gtgatgggca tggacgagct gtacaagtaa
     6061 tagtctagag gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac
     6121 tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta
     6181 aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg
     6241 agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta
     6301 gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag aagagaaagg
     6361 tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat gctgtgttta
     6421 gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct gcactgctat
     6481 acaagaaaat tatggaaaaa tatttgatgt atagtgcctt gactagagat cataatcagc
     6541 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     6601 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     6661 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     6721 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcca ctagtgtcga
     6781 cgatgtaggt cacggtctcg aagccgcggt gcgggtgcca gggcgtgccc ttgggctccc
     6841 cgggcgcgta ctccacctca cccatctggt ccatcatgat gaacgggtcg aggtggcggt
     6901 agttgatccc ggcgaacgcg cggcgcaccg ggaagccctc gccctcgaaa ccgctgggcg
     6961 cggtggtcac ggtgagcacg ggacgtgcga cggcgtcggc gggtgcggat acgcggggca
     7021 gcgtcagcgg gttctcgacg gtcacggcgg gcatgtcgac actagttcta gccagctttt
     7081 gttcccttta gtgagggtta atttcgagct tggcgtaatc atggtcatag ctgtttcctg
     7141 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     7201 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     7261 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     7321 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     7381 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     7441 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     7501 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     7561 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     7621 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     7681 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     7741 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     7801 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     7861 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     7921 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     7981 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     8041 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     8101 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     8161 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     8221 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     8281 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     8341 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     8401 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     8461 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     8521 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     8581 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     8641 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     8701 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     8761 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     8821 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     8881 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     8941 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     9001 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     9061 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     9121 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     9181 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     9241 gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt
     9301 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     9361 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     9421 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     9481 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     9541 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     9601 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     9661 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     9721 acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     9781 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     9841 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata
     9901 cgactcacta tagggcgaat tgggtacgta ccgggcccct agtatgtatg taagttaata
     9961 aaacccattt ttgcggaaag tagataaaaa aaacattttt tttttttact gcactggata
    10021 tcattgaact tatctgatca gttttaaatt tacttcgatc caagggtatt tgatgtacca
    10081 ggttctttcg attacctctc actcaaaatg acattccact caaagtcagc gctgtttgcc
    10141 tccttctctg tccacagaaa tatcgccgtc tctttcgccg ctgcgtccgc tatctctttc
    10201 gccaccgttt gtagcgttac gtagcgtcaa tgtccgcctt cagttgcatt ttgtcagcgg
    10261 tttcgtgacg aagctccaag cggtttacgc catcaattaa acacaaagtg ctgtgccaaa
    10321 actcctctcg cttcttattt ttgtttgttt tttgagtgat tggggtggtg attggttttg
    10381 ggtgggtaag caggggaaag tgtgaaaaat cccggcaatg ggccaagagg atcaggagct
    10441 attaattcgc ggaggcagca aacacccatc tgccgagcat ctgaacaatg tgagtagtac
    10501 atgtgcatac atcttaagtt cacttgatct ataggaactg cgattgcaac atcaaattgt
    10561 ctgcggcgtg agaactgcga cccacaaaaa tcccaaaccg caattgcaca aacaaatagt
    10621 gacacgaaac agattattct ggtagctgtt ctcgctatat aagacaattt ttgagatcat
    10681 atcatgatca agacatctaa aggcattcat tttcgactat attctttttt acaaaaaata
    10741 taacaaccag atattttaag ctgatcctag atgcacaaaa aataaataaa agtataaacc
    10801 tacttcgtag gatacttcgg ggtacttttt gttcggggtt agatgagcat aacgcttgta
    10861 gttgatattt gagatcccct atcattgcag ggtgacagcg gagcggcttc gcagagctgc
    10921 attaaccagg gcttcgggca ggccaaaaac tacggcacgc tccggccacc cagtccgccg
    10981 gaggactccg gttcagggag cggccaacta gccgagaacc tcacctatgc ctggcacaat
    11041 atggacatct ttggggcggt caatcagccg ggctccggat ggcggcagct ggtcaaccgg
    11101 acacgcggac tattctgcaa cgagcgacac ataccggcgc ccaggaaaca tttgctcaag
    11161 aacggtgagt ttctattcgc agtcggctga tctgtgtgaa atcttaataa agggtccaat
    11221 taccaatttg aaactcagtt tgcggcgtgg cctatccggg cgaacttttg gccgtgatgg
    11281 gcagttccgg tgccggaaag acgaccctgc tgaatgccct tgcctttcga tcgccgcagg
    11341 gcatccaagt atcgccatcc gggatgcgac tgctcaatgg ccaacctgtg gacgccaagg
    11401 agatgcaggc caggtgcgcc tatgtccagc aggatgacct ctttatcggc tccctaacgg
    11461 ccagggaaca cctgattttc caagccatgg tgcggatgcc acgacatctg acctatcggc
    11521 agcgagtggc ccgcgtggat caggtgatcc aggagctttc gctcagcaaa tgtcagcaca
    11581 cgatcatcgg tgtgcccggc agggtgaaag gtctgtccgg cggagaaagg aagcgtctgg
    11641 cattcgcctc cgaggctcta accgatccgc cgcttctgat ctgcgatgag cccacctccg
    11701 gactggactc ctttaccgcc cacagcgtcg tccaggtgct gaagaagctg tcgcagaagg
    11761 gcaagaccgt catcctgacc attcatcagc cgtcttccga gctgtttgag ctctttgaca
    11821 agatccttct gatggccgag ggcagggtag ctttcttggg cactcccagc gaagccgtcg
    11881 acttcttttc ctagtgagtt cgatgtgttt attaagggta tctagtatta cataacatct
    11941 caactcctat ccagcgtggg tgcccagtgt cctaccaact acaatccggc ggacttttac
    12001 gtacaggtgt tggccgttgt gcccggacgg gagatcgagt cccgtgatcg gatcgccaag
    12061 atatgcgaca attttgccat tagcaaagta gcccgggata tggagcagtt gttggccacc
    12121 aaaaatctgg agaagccact ggagcagccg gagaatgggt acacctacaa ggccacctgg
    12181 ttcatgcagt tccgggcggt cctgtggcga tcctggctgt cggtgctcaa ggaaccactc
    12241 ctcgtaaaag tgcgacttat tcagacaacg gtgagtggtt ccagtggaaa caaatgatat
    12301 aacgcttaca attcttggaa acaaattcgc tagattttag atagaattgc ctgattccac
    12361 acccttctta gtttttttca atgagatgta tagtttatag ttttgcagaa gataaataaa
    12421 tttcatttaa ctcgcgaata ttaatgagat gcgagtaaca ttttaatttg cagatggttg
    12481 ccatcttgat tggcctcatc tttttgggcc aacaactcac gcaagtgggt gtgatgaata
    12541 tcaacggagc catcttcctc ttcctgacca acatgacctt tcaaaacgtc tttgccacga
    12601 taaatgtaag tcatgtttag aatacatttg catttcaata atttactaac tttctaatga
    12661 atcgattcga tttaggtgtt cacctcagag ctgccagttt ttatgaggga ggcccgaagt
    12721 cgactttatc gctgtgacac atactttctg ggcaaaacga ttgccgaatt gccgcttttt
    12781 ctcacagtgc cactggtctt cacggcgatt gcctatccga tgatcggact gcgggccgga
    12841 gtgctgcact tcttcaactg cctggcgctg gtcactctgg tggccaatgt gtcaacgtcc
    12901 ttcggatatc taatatcctg cgccagctcc tcgacctcga tggcgctgtc tgtgggtccg
    12961 ccggttatca taccattcct gctctttggc ggcttcttct tgaactcggg ctcggtgcca
    13021 gtatacctca aatggttgtc gtacctctca tggttccgtt acgccaacga gggtctgctg
    13081 attaaccaat gggcggacgt ggagccgggc gaaattagct gcacatcgtc gaacaccacg
    13141 tgccccagtt cgggcaaggt catcctggag acgcttaact tctccgccgc cgatctgccg
    13201 ctggactacg tgggtctggc cattctcatc gtgagcttcc gggtgctcgc atatctggct
    13261 ctaagacttc gggcccgacg caaggagtag ccgacatata tccgaaataa ctgcttgttt
    13321 ttttttttta ccattattac catcgtgttt actgtttatt gccccctcaa aaagctaatg
    13381 taattatatt tgtgccaata aaaacaagat atgacctata gaatacaagt atttcccctt
    13441 cgaacatccc cacaagtaga ctttggattt gtcttctaac caaaagactt acacacctgc
    13501 ataccttaca tcaaaaactc gtttatcgct acataaaaca ccgggatata ttttttatat
    13561 acatactttt caaatcgcgc gccctcttca taattcacct ccaccacacc acgtttcgta
    13621 gttgctcttt cgctgtctcc cacccgctct ccgcaacaca ttcacctttt gttcgacgac
    13681 cttggagcga ctgtcgttag ttccgcgcga ttcggttcgc tcaaatggtt ccgagtggtt
    13741 catttcgtct caatagaaat tagtaataaa tatttgtatg tacaatttat ttgctccaat
    13801 atatttgtat atatttccct cacagctata tttattctaa tttaatatta tgacttttta
    13861 aggtaatttt ttgtgacctg ttcggagtga ttagcgttac aatttgaact gaaagtgaca
    13921 tccagtgttt gttccttgtg tagatgcatc tcaaaaaaat ggtgggcata atagtgttgt
    13981 ttatatatat caaaaataac aactataata ataagaatac attta
//
