LOCUS       sequence-274787-       14025 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used to generate a transgene that will express an inactive 
            fluorescent Chitin Synthase in Drosophila using Gal4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14025)
  AUTHORS   Adler PN
  TITLE     The localization of chitin synthase mediates the patterned 
            deposition of chitin in developing Drosophila bristles
  JOURNAL   bioRxiv 2020
REFERENCE   2  (bases 1 to 14025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2020"
COMMENT     Sequence Label: pUAS-kkv-R896K::NG
FEATURES             Location/Qualifiers
     source          1..14025
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     55..76
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             complement(835..846)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      2130..2139
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5001..5711
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      5256..5265
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          5798..5863
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5993..6013
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6285..6419
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6322..6341)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6376..6395
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    6448..6517
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(6736..6754)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6775..6791)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6775..6791)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6788..6810)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6799..6815
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6823..6829,6830..6847,6848..6853))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6868..6889
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7006..7023)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7177..7765)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7257..7276)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7936..8727,8728..8796))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8559..8578
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8797..8901)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8927..9382)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9064..9085)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9276..9295
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9509..9531
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9523..9540
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9524..9540
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9547..9566
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9547..9565
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9599..9617)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(10069..10140,10542..10815,10890..11544,11606..11921,
                     12125..12256,12327..12941)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    13716..13749
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    13782..13876
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        join(13895..14025,1..108)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
ORIGIN
        1 agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac caagtaaatc aactgcaact
       61 actgaaatct gccaagaagt aattattgaa tacaagaaga gaactctgaa tagggaattg
      121 ggaattcgtt aacagatctg cggccgcggc tcgagatgtc tgcgatgcgg catcgcccga
      181 tggcccctcc gggccaagga ccaggagcgg gaaccgctgg ggagcacgtc gacagcgatg
      241 acaacaactt taccgacgac gagagctcgc ccctcaccca cgacatttac ggcggcagcc
      301 aacgcaccat acaagagacg aagggttggg atgttttccg ggatccgcca atcaaaattg
      361 agaccggatc gacggcgaac caggaatgct tagaattaac cgtgaagatc ttgaagatct
      421 tcgcctatgt gattacgttt ataatagttt taaccggtgg cgtcatcgcc aagggcacaa
      481 tgctcttcat gacttcgcag gtgcgcaagg ataagaagat ggagtactgc aacaaagact
      541 tgggtcggga caaaagcttc gtagtccgac ttccggagga ggagcgagtg gcctggatct
      601 gggctctgct catagcatat gcccttcccg agatcggagc cttgatccga tccgcccgca
      661 tctgcttctt caagaccttt aaggtgccga agacgggcca cttcctcttc gtctggctga
      721 tggagagctt gagtgccgtg ggcatggcgc tgctgatgtt cgtggttctg ccccagatcg
      781 acgccatcca gggcgccatg ctaaccaact gcctgtgcgt cgtgccgggc atcttcggcc
      841 ttctatcgcg cacctccaag gagggcaagc gctttgtaaa ggtgatcatc gatctggcgg
      901 cggtggcggc ccaggtgacg ggcctggtga tctggccgct gctggagaac cgccgtgagc
      961 tgtgggtcat cccggtggcc tgtgtcatga tctcgtgcgg ctggtgggag aattacgtgt
     1021 cgccacagtc cccgctcggc ctggttcgag ccctgggtcg catcaaggag gagatgaagt
     1081 acactcgcta cttctgccac atattcctat ccatctggaa gatcctgctt ttcttcaccg
     1141 tcacactgct catctactgg gcgcaaggcg aggaacccgg caatctgttt gccatgtacg
     1201 gagatgcctt tggaccccac aagatcatag tctacgaact gccagcggga ctgggcggtg
     1261 tgcttccaga taccctggaa tcggccaaca ttgacaccgt ggatgtggat gccgcctaca
     1321 atacggtggt gtatgtactg cttctacaga ttttcggcgc ctacttgtgc tacatcttcg
     1381 gaaagttcgc ctgcaagatc ctcatccagg gattcagcta cgcgtttccc gtcagtctaa
     1441 ctgttccgct ctctgttacg ttcctgatcg cagcctgtgg aatccggatc gacgatccct
     1501 gcttcttcca tgacaccatt ccggactatc tgttcttcac gagcccctcg aacttccggt
     1561 tcaacaactt tgtcaccgag caaatggcgt gggcctggat cctttggctc ctcagtcaga
     1621 cctggatagc actgcacatc tggactccga agtgcgagcg tctggccacc accgagaagt
     1681 tgtttgtcca gcccatgtac tcctctctac tgatcgatca gtcgatggcc ctcaaccgaa
     1741 ggcgcgatga ccaggcagat gtgaaaacag aggatctctc ggagatcgaa aaggaaaagg
     1801 gcgatgagta ctacgagacc atttctgtgc acacggatcg ctcctcggca cccaacaagc
     1861 catcgattaa gtcctccgac aacatcacac gaatctactc ctgcgcgacc atgtggcatg
     1921 agacgaagga cgagatgata gagttcttga agagcatcat gcgaatggac gaggaccagt
     1981 gtgctcgtcg cgtggctcaa aagtatctga gggttctcga tccggactat tatgaatttg
     2041 agacccacat cttcttcgat gacgccttcg aaatctccga tcacagcgac gatgatatcc
     2101 agtgcaatcg attcgtcaag ctcttgatag ccaccatgga cgaggctgcc tccgagatcc
     2161 atcagaccac gatcagactg cgtccgccca agaagtatcc cactccgtac ggaggccgcc
     2221 tggtgtggac ccttccaggg aagaccaaat tcattacgca cttaaaggac aaggaccgca
     2281 ttcgtcacag gaagcgttgg tctcaggtca tgtacatgta ttacctgctg ggacatcgtc
     2341 taatggagct gcccatttcg gtggatcgca aggatgcaat tgcggagaac acctaccttt
     2401 tgaccctgga cggagatatt gacttcaagc cgaatgcggt gactctactg gtggatctga
     2461 tgaagaagaa caagaacctg ggtgctgcct gcggtcgcat tcatcccgtg ggatcgggac
     2521 ccatggtgtg gtaccaactt ttcgaatacg ccatcggcca ttggctgcaa aaggccacgg
     2581 agcacatgat tggctgcgtg ctctgttcac ctggatgctt ctccctgttc aggggcaagg
     2641 ccctcatgga tgacaatgtg atgaagaaat acacgacacg gtcggatgag gctcgtcact
     2701 acgtgcagta cgatcagggc gaggatcgtt ggctgtgcac attgctcctc cagaggggat
     2761 accgcgtgga gtactcggct gccagtgatg cgtacaccca ctgccccgag ggattcaatg
     2821 agttctacaa ccagcggcgc aaatgggtgc cctcgaccat agccaacatc atggatcttc
     2881 tggcggatgc gaagcgcacg atcaagatca atgacaacat ttcgcttctg tacatcttct
     2941 accagatgat gttgatgggc ggcaccatcc tgggacccgg aaccattttc cttatgttgg
     3001 tgggtgcatt cgtggcagcc tttcgcattg acaactggac ctccttccac tacaacatcg
     3061 tgccgatcct ggccttcatg tttatctgct tcacctgtaa gtcgaacatc cagttgttcg
     3121 tggcccaagt cctttcgacg gcatatgccc tgatcatgat ggcggtgatt gtgggtacgg
     3181 ccttgcagtt gggagaggac ggaataggct ccccttcggc cattttcctg atatccatgg
     3241 tgggctcatt cttcatagcc gcatgtttgc atccacaaga gttctggtgc attacatgcg
     3301 gcctaatcta tttgctgtcc atcccgtcca tgtacctgct gctcatcttg tactctatca
     3361 tcaacctaaa cgtcgtctcc tggggcaccc gcgaggtggt ggctaagaag accaagaaag
     3421 aactggaggc ggagaagaag gccgccgagg aggccaaaaa gagggtgaag cagaagagca
     3481 tgctgagctt ccttcagagt ggaatcgggg acaatggcga cgaggagggc tccgtggagt
     3541 tctctctcgc tggactcttc cgctgcatat tctgcaccca cggaaaaaca tccgacgaga
     3601 agcagcagct gacctccatc gccgaatcgc tggacacgat caaacatcga atggacacca
     3661 tcgagagtgc tgtggatccc catggtcacc atgcctcccg acatggcagg aggagaacca
     3721 catccagtgg ctccaaggat caccacctgc tgacctcggt ggcggagaag tctggcgatg
     3781 aatcggacga gtcggactca gacacttcgg cggaaccaaa gcaggagcga gactttctaa
     3841 ccaatcccta ctggatcgag gaccccgatg tacgcaaggg cgaggtcgat tttctctcca
     3901 gcaccgagat ccagttctgg aaggatctca tcgaccagta tctctatccc atcgacaatg
     3961 atcccgtgga gcaggcccgc atagccaaag atctgaagga gctgcgcgac tcgtcggtgt
     4021 tcgcgttctt catgatcaat gcactgttcg tgctgatcgt gttcctgctg cagctgaaca
     4081 aggacaacat ccacgtgaag tggcccttcg gagtgcggac gaacatcacc tacgacgagt
     4141 ccacccagga ggtgcacatc tccaaggagt accttcagct ggagcccatc ggattggtgt
     4201 tcgtattctt ctttgctctt attttgataa tccagttcac ggccatgctg ttccatcgat
     4261 ttggaaccat ttcgcacatc ctggcctcga ctgaactcaa cttctgcaag aagaagtctg
     4321 aggatctgac acaggaccaa ctaatagata agcatgctgt ggagatcgtc aagaacttgc
     4381 agaggctgca gggaatcgat ggcgactacg acaatgactc cggttccggg ccagatcgca
     4441 tagccaggcg gaagaccatt caaaatctgg agaaggcgcg acagccacgt cgccagatcg
     4501 gcaccctgga tgtggcgttc aagaagcgct tcctgaaact caccgccgat gcggagaaca
     4561 atccggccac gcccattctc acccgccgcc tgacaatgcg ggcggagacc atccgggcac
     4621 tggaggtgcg taagaattcg gtgatggccg aaaggcggaa gtccgcgatg caaactctgg
     4681 gagcaaagaa cgagtacgga atcacaactg gagcaccgat taacaacaat ggagctctgc
     4741 cgaatcagcg aagcggacgt gtctcgaatg caggcatcag catcaaggat gtattcaatg
     4801 tgaacggtgg tggtgccgag cagatttacg gctcgaatgg gggcggcact atcaaccagg
     4861 gttacgagca tgtgatcgac gaggacggcg acggcaactc cctgcggctg accaccagga
     4921 atccccatcc ccacccgcac caccaggtct cctggggcca gaacaccaac ggcggcggcg
     4981 gtaatggcac aggtcgcctg atggtgagca agggcgagga ggataacatg gcctctctcc
     5041 cagcgacaca tgagttacac atctttggct ccatcaacgg tgtggacttt gacatggtgg
     5101 gtcagggcac cggcaatcca aatgatggtt atgaggagtt aaacctgaag tccaccaagg
     5161 gtgacctcca gttctccccc tggattctgg tccctcatat cgggtatggc ttccatcagt
     5221 acctgcccta ccctgacggg atgtcgcctt tccaggccgc catggtagat ggctccggat
     5281 accaagtcca tcgcacaatg cagtttgaag atggtgcctc ccttactgtt aactaccgct
     5341 acacctacga gggaagccac atcaaaggag aggcccaggt gaaggggact ggtttccctg
     5401 ctgacggtcc tgtgatgacc aactcgctga ccgctgcgga ctggtgcagg tcgaagaaga
     5461 cttaccccaa cgacaaaacc atcatcagta cctttaagtg gagttacacc actggaaatg
     5521 gcaagcgcta ccggagcact gcgcggacca cctacacctt tgccaagcca atggcggcta
     5581 actatctgaa gaaccagccg atgtacgtgt tccgtaagac ggagctcaag cactccaaga
     5641 ccgagctcaa cttcaaggag tggcaaaagg cctttaccga tgtgatgggc atggacgagc
     5701 tgtacaagta atagtctaga ggatctttgt gaaggaacct tacttctgtg gtgtgacata
     5761 attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt
     5821 ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac
     5881 tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga
     5941 aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa
     6001 gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca
     6061 tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc
     6121 tgcactgcta tacaagaaaa ttatggaaaa atatttgatg tatagtgcct tgactagaga
     6181 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     6241 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     6301 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     6361 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc
     6421 actagtgtcg acgatgtagg tcacggtctc gaagccgcgg tgcgggtgcc agggcgtgcc
     6481 cttgggctcc ccgggcgcgt actccacctc acccatctgg tccatcatga tgaacgggtc
     6541 gaggtggcgg tagttgatcc cggcgaacgc gcggcgcacc gggaagccct cgccctcgaa
     6601 accgctgggc gcggtggtca cggtgagcac gggacgtgcg acggcgtcgg cgggtgcgga
     6661 tacgcggggc agcgtcagcg ggttctcgac ggtcacggcg ggcatgtcga cactagttct
     6721 agccagcttt tgttcccttt agtgagggtt aatttcgagc ttggcgtaat catggtcata
     6781 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     6841 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     6901 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     6961 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     7021 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     7081 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     7141 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     7201 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     7261 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     7321 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     7381 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     7441 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     7501 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     7561 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     7621 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     7681 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     7741 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     7801 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     7861 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     7921 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     7981 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     8041 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     8101 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     8161 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     8221 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     8281 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     8341 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     8401 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     8461 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     8521 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     8581 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     8641 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     8701 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     8761 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     8821 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     8881 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt
     8941 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     9001 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     9061 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     9121 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     9181 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     9241 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     9301 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     9361 aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg
     9421 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
     9481 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
     9541 gaattgtaat acgactcact atagggcgaa ttgggtacgt accgggcccc tagtatgtat
     9601 gtaagttaat aaaacccatt tttgcggaaa gtagataaaa aaaacatttt ttttttttac
     9661 tgcactggat atcattgaac ttatctgatc agttttaaat ttacttcgat ccaagggtat
     9721 ttgatgtacc aggttctttc gattacctct cactcaaaat gacattccac tcaaagtcag
     9781 cgctgtttgc ctccttctct gtccacagaa atatcgccgt ctctttcgcc gctgcgtccg
     9841 ctatctcttt cgccaccgtt tgtagcgtta cgtagcgtca atgtccgcct tcagttgcat
     9901 tttgtcagcg gtttcgtgac gaagctccaa gcggtttacg ccatcaatta aacacaaagt
     9961 gctgtgccaa aactcctctc gcttcttatt tttgtttgtt ttttgagtga ttggggtggt
    10021 gattggtttt gggtgggtaa gcaggggaaa gtgtgaaaaa tcccggcaat gggccaagag
    10081 gatcaggagc tattaattcg cggaggcagc aaacacccat ctgccgagca tctgaacaat
    10141 gtgagtagta catgtgcata catcttaagt tcacttgatc tataggaact gcgattgcaa
    10201 catcaaattg tctgcggcgt gagaactgcg acccacaaaa atcccaaacc gcaattgcac
    10261 aaacaaatag tgacacgaaa cagattattc tggtagctgt tctcgctata taagacaatt
    10321 tttgagatca tatcatgatc aagacatcta aaggcattca ttttcgacta tattcttttt
    10381 tacaaaaaat ataacaacca gatattttaa gctgatccta gatgcacaaa aaataaataa
    10441 aagtataaac ctacttcgta ggatacttcg gggtactttt tgttcggggt tagatgagca
    10501 taacgcttgt agttgatatt tgagatcccc tatcattgca gggtgacagc ggagcggctt
    10561 cgcagagctg cattaaccag ggcttcgggc aggccaaaaa ctacggcacg ctccggccac
    10621 ccagtccgcc ggaggactcc ggttcaggga gcggccaact agccgagaac ctcacctatg
    10681 cctggcacaa tatggacatc tttggggcgg tcaatcagcc gggctccgga tggcggcagc
    10741 tggtcaaccg gacacgcgga ctattctgca acgagcgaca cataccggcg cccaggaaac
    10801 atttgctcaa gaacggtgag tttctattcg cagtcggctg atctgtgtga aatcttaata
    10861 aagggtccaa ttaccaattt gaaactcagt ttgcggcgtg gcctatccgg gcgaactttt
    10921 ggccgtgatg ggcagttccg gtgccggaaa gacgaccctg ctgaatgccc ttgcctttcg
    10981 atcgccgcag ggcatccaag tatcgccatc cgggatgcga ctgctcaatg gccaacctgt
    11041 ggacgccaag gagatgcagg ccaggtgcgc ctatgtccag caggatgacc tctttatcgg
    11101 ctccctaacg gccagggaac acctgatttt ccaagccatg gtgcggatgc cacgacatct
    11161 gacctatcgg cagcgagtgg cccgcgtgga tcaggtgatc caggagcttt cgctcagcaa
    11221 atgtcagcac acgatcatcg gtgtgcccgg cagggtgaaa ggtctgtccg gcggagaaag
    11281 gaagcgtctg gcattcgcct ccgaggctct aaccgatccg ccgcttctga tctgcgatga
    11341 gcccacctcc ggactggact cctttaccgc ccacagcgtc gtccaggtgc tgaagaagct
    11401 gtcgcagaag ggcaagaccg tcatcctgac cattcatcag ccgtcttccg agctgtttga
    11461 gctctttgac aagatccttc tgatggccga gggcagggta gctttcttgg gcactcccag
    11521 cgaagccgtc gacttctttt cctagtgagt tcgatgtgtt tattaagggt atctagtatt
    11581 acataacatc tcaactccta tccagcgtgg gtgcccagtg tcctaccaac tacaatccgg
    11641 cggactttta cgtacaggtg ttggccgttg tgcccggacg ggagatcgag tcccgtgatc
    11701 ggatcgccaa gatatgcgac aattttgcca ttagcaaagt agcccgggat atggagcagt
    11761 tgttggccac caaaaatctg gagaagccac tggagcagcc ggagaatggg tacacctaca
    11821 aggccacctg gttcatgcag ttccgggcgg tcctgtggcg atcctggctg tcggtgctca
    11881 aggaaccact cctcgtaaaa gtgcgactta ttcagacaac ggtgagtggt tccagtggaa
    11941 acaaatgata taacgcttac aattcttgga aacaaattcg ctagatttta gatagaattg
    12001 cctgattcca cacccttctt agtttttttc aatgagatgt atagtttata gttttgcaga
    12061 agataaataa atttcattta actcgcgaat attaatgaga tgcgagtaac attttaattt
    12121 gcagatggtt gccatcttga ttggcctcat ctttttgggc caacaactca cgcaagtggg
    12181 tgtgatgaat atcaacggag ccatcttcct cttcctgacc aacatgacct ttcaaaacgt
    12241 ctttgccacg ataaatgtaa gtcatgttta gaatacattt gcatttcaat aatttactaa
    12301 ctttctaatg aatcgattcg atttaggtgt tcacctcaga gctgccagtt tttatgaggg
    12361 aggcccgaag tcgactttat cgctgtgaca catactttct gggcaaaacg attgccgaat
    12421 tgccgctttt tctcacagtg ccactggtct tcacggcgat tgcctatccg atgatcggac
    12481 tgcgggccgg agtgctgcac ttcttcaact gcctggcgct ggtcactctg gtggccaatg
    12541 tgtcaacgtc cttcggatat ctaatatcct gcgccagctc ctcgacctcg atggcgctgt
    12601 ctgtgggtcc gccggttatc ataccattcc tgctctttgg cggcttcttc ttgaactcgg
    12661 gctcggtgcc agtatacctc aaatggttgt cgtacctctc atggttccgt tacgccaacg
    12721 agggtctgct gattaaccaa tgggcggacg tggagccggg cgaaattagc tgcacatcgt
    12781 cgaacaccac gtgccccagt tcgggcaagg tcatcctgga gacgcttaac ttctccgccg
    12841 ccgatctgcc gctggactac gtgggtctgg ccattctcat cgtgagcttc cgggtgctcg
    12901 catatctggc tctaagactt cgggcccgac gcaaggagta gccgacatat atccgaaata
    12961 actgcttgtt tttttttttt accattatta ccatcgtgtt tactgtttat tgccccctca
    13021 aaaagctaat gtaattatat ttgtgccaat aaaaacaaga tatgacctat agaatacaag
    13081 tatttcccct tcgaacatcc ccacaagtag actttggatt tgtcttctaa ccaaaagact
    13141 tacacacctg cataccttac atcaaaaact cgtttatcgc tacataaaac accgggatat
    13201 attttttata tacatacttt tcaaatcgcg cgccctcttc ataattcacc tccaccacac
    13261 cacgtttcgt agttgctctt tcgctgtctc ccacccgctc tccgcaacac attcaccttt
    13321 tgttcgacga ccttggagcg actgtcgtta gttccgcgcg attcggttcg ctcaaatggt
    13381 tccgagtggt tcatttcgtc tcaatagaaa ttagtaataa atatttgtat gtacaattta
    13441 tttgctccaa tatatttgta tatatttccc tcacagctat atttattcta atttaatatt
    13501 atgacttttt aaggtaattt tttgtgacct gttcggagtg attagcgtta caatttgaac
    13561 tgaaagtgac atccagtgtt tgttccttgt gtagatgcat ctcaaaaaaa tggtgggcat
    13621 aatagtgttg tttatatata tcaaaaataa caactataat aataagaata catttaattt
    13681 agaaaatgct tggatttcac tggaactagg ctagcataac ttcgtataat gtatgctata
    13741 cgaagttatg ctagcggatc caagcttgca tgcctgcagg tcggagtact gtcctccgag
    13801 cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg
    13861 agtactgtcc tccgagcgga gactctagcg agcgccggag tataaataga ggcgcttcgt
    13921 ctacggagcg acaattcaat tcaaacaagc aaagtgaaca cgtcgctaag cgaaagctaa
    13981 gcaaataaac aagcgcagct gaacaagcta aacaatctgc agtaa
//
