LOCUS       sequence-274788-       14025 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used to generate a transgene that will express a fluorescent Chitin 
            Synthase in Drosophila using Gal4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14025)
  AUTHORS   Adler PN
  TITLE     The localization of chitin synthase mediates the patterned 
            deposition of chitin in developing Drosophila bristles
  JOURNAL   bioRxiv 2020
REFERENCE   2  (bases 1 to 14025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2020"
COMMENT     Sequence Label: pUAS-kkv::NG
FEATURES             Location/Qualifiers
     source          1..14025
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    66..99
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    132..226
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        245..483
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     430..451
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             complement(1210..1221)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      2505..2514
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5376..6086
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      5631..5640
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          6173..6238
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6368..6388
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6660..6794
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6697..6716)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6751..6770
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    6823..6892
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(7111..7129)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7150..7166)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7150..7166)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7163..7185)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7174..7190
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7198..7204,7205..7222,7223..7228))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7243..7264
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7381..7398)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7552..8140)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7632..7651)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8311..9102,9103..9171))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8934..8953
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9172..9276)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9302..9757)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9439..9460)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9651..9670
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9884..9906
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9898..9915
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9899..9915
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9922..9941
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9922..9940
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9974..9992)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(10444..10515,10917..11190,11265..11919,11981..12296,
                     12500..12631,12702..13316)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
ORIGIN
        1 caactataat aataagaata catttaattt agaaaatgct tggatttcac tggaactagg
       61 ctagcataac ttcgtataat gtatgctata cgaagttatg ctagcggatc caagcttgca
      121 tgcctgcagg tcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt
      181 cctccgagcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gactctagcg
      241 agcgccggag tataaataga ggcgcttcgt ctacggagcg acaattcaat tcaaacaagc
      301 aaagtgaaca cgtcgctaag cgaaagctaa gcaaataaac aagcgcagct gaacaagcta
      361 aacaatctgc agtaaagtgc aagttaaagt gaatcaatta aaagtaacca gcaaccaagt
      421 aaatcaactg caactactga aatctgccaa gaagtaatta ttgaatacaa gaagagaact
      481 ctgaataggg aattgggaat tcgttaacag atctgcggcc gcggctcgag atgtctgcga
      541 tgcggcatcg cccgatggcc cctccgggcc aaggaccagg agcgggaacc gctggggagc
      601 acgtcgacag cgatgacaac aactttaccg acgacgagag ctcgcccctc acccacgaca
      661 tttacggcgg cagccaacgc accatacaag agacgaaggg ttgggatgtt ttccgggatc
      721 cgccaatcaa aattgagacc ggatcgacgg cgaaccagga atgcttagaa ttaaccgtga
      781 agatcttgaa gatcttcgcc tatgtgatta cgtttataat agttttaacc ggtggcgtca
      841 tcgccaaggg cacaatgctc ttcatgactt cgcaggtgcg caaggataag aagatggagt
      901 actgcaacaa agacttgggt cgggacaaaa gcttcgtagt ccgacttccg gaggaggagc
      961 gagtggcctg gatctgggct ctgctcatag catatgccct tcccgagatc ggagccttga
     1021 tccgatccgc ccgcatctgc ttcttcaaga cctttaaggt gccgaagacg ggccacttcc
     1081 tcttcgtctg gctgatggag agcttgagtg ccgtgggcat ggcgctgctg atgttcgtgg
     1141 ttctgcccca gatcgacgcc atccagggcg ccatgctaac caactgcctg tgcgtcgtgc
     1201 cgggcatctt cggccttcta tcgcgcacct ccaaggaggg caagcgcttt gtaaaggtga
     1261 tcatcgatct ggcggcggtg gcggcccagg tgacgggcct ggtgatctgg ccgctgctgg
     1321 agaaccgccg tgagctgtgg gtcatcccgg tggcctgtgt catgatctcg tgcggctggt
     1381 gggagaatta cgtgtcgcca cagtccccgc tcggcctggt tcgagccctg ggtcgcatca
     1441 aggaggagat gaagtacact cgctacttct gccacatatt cctatccatc tggaagatcc
     1501 tgcttttctt caccgtcaca ctgctcatct actgggcgca aggcgaggaa cccggcaatc
     1561 tgtttgccat gtacggagat gcctttggac cccacaagat catagtctac gaactgccag
     1621 cgggactggg cggtgtgctt ccagataccc tggaatcggc caacattgac accgtggatg
     1681 tggatgccgc ctacaatacg gtggtgtatg tactgcttct acagattttc ggcgcctact
     1741 tgtgctacat cttcggaaag ttcgcctgca agatcctcat ccagggattc agctacgcgt
     1801 ttcccgtcag tctaactgtt ccgctctctg ttacgttcct gatcgcagcc tgtggaatcc
     1861 ggatcgacga tccctgcttc ttccatgaca ccattccgga ctatctgttc ttcacgagcc
     1921 cctcgaactt ccggttcaac aactttgtca ccgagcaaat ggcgtgggcc tggatccttt
     1981 ggctcctcag tcagacctgg atagcactgc acatctggac tccgaagtgc gagcgtctgg
     2041 ccaccaccga gaagttgttt gtccagccca tgtactcctc tctactgatc gatcagtcga
     2101 tggccctcaa ccgaaggcgc gatgaccagg cagatgtgaa aacagaggat ctctcggaga
     2161 tcgaaaagga aaagggcgat gagtactacg agaccatttc tgtgcacacg gatcgctcct
     2221 cggcacccaa caagccatcg attaagtcct ccgacaacat cacacgaatc tactcctgcg
     2281 cgaccatgtg gcatgagacg aaggacgaga tgatagagtt cttgaagagc atcatgcgaa
     2341 tggacgagga ccagtgtgct cgtcgcgtgg ctcaaaagta tctgagggtt ctcgatccgg
     2401 actattatga atttgagacc cacatcttct tcgatgacgc cttcgaaatc tccgatcaca
     2461 gcgacgatga tatccagtgc aatcgattcg tcaagctctt gatagccacc atggacgagg
     2521 ctgcctccga gatccatcag accacgatca gactgcgtcc gcccaagaag tatcccactc
     2581 cgtacggagg ccgcctggtg tggacccttc cagggaagac caaattcatt acgcacttaa
     2641 aggacaagga ccgcattcgt cacaggaagc gttggtctca ggtcatgtac atgtattacc
     2701 tgctgggaca tcgtctaatg gagctgccca tttcggtgga tcgcaaggat gcaattgcgg
     2761 agaacaccta ccttttgacc ctggacggag atattgactt caagccgaat gcggtgactc
     2821 tactggtgga tctgatgaag aagaacaaga acctgggtgc tgcctgcggt cgcattcatc
     2881 ccgtgggatc gggacccatg gtgtggtacc aacttttcga atacgccatc ggccattggc
     2941 tgcaaaaggc cacggagcac atgattggct gcgtgctctg ttcacctgga tgcttctccc
     3001 tgttcagggg caaggccctc atggatgaca atgtgatgaa gaaatacacg acacggtcgg
     3061 atgaggctcg tcactacgtg cagtacgatc agggcgagga tcgttggctg tgcacattgc
     3121 tcctccagag gggataccgc gtggagtact cggctgccag tgatgcgtac acccactgcc
     3181 ccgagggatt caatgagttc tacaaccagc ggcgcagatg ggtgccctcg accatagcca
     3241 acatcatgga tcttctggcg gatgcgaagc gcacgatcaa gatcaatgac aacatttcgc
     3301 ttctgtacat cttctaccag atgatgttga tgggcggcac catcctggga cccggaacca
     3361 ttttccttat gttggtgggt gcattcgtgg cagcctttcg cattgacaac tggacctcct
     3421 tccactacaa catcgtgccg atcctggcct tcatgtttat ctgcttcacc tgtaagtcga
     3481 acatccagtt gttcgtggcc caagtccttt cgacggcata tgccctgatc atgatggcgg
     3541 tgattgtggg tacggccttg cagttgggag aggacggaat aggctcccct tcggccattt
     3601 tcctgatatc catggtgggc tcattcttca tagccgcatg tttgcatcca caagagttct
     3661 ggtgcattac atgcggccta atctatttgc tgtccatccc gtccatgtac ctgctgctca
     3721 tcttgtactc tatcatcaac ctaaacgtcg tctcctgggg cacccgcgag gtggtggcta
     3781 agaagaccaa gaaagaactg gaggcggaga agaaggccgc cgaggaggcc aaaaagaggg
     3841 tgaagcagaa gagcatgctg agcttccttc agagtggaat cggggacaat ggcgacgagg
     3901 agggctccgt ggagttctct ctcgctggac tcttccgctg catattctgc acccacggaa
     3961 aaacatccga cgagaagcag cagctgacct ccatcgccga atcgctggac acgatcaaac
     4021 atcgaatgga caccatcgag agtgctgtgg atccccatgg tcaccatgcc tcccgacatg
     4081 gcaggaggag aaccacatcc agtggctcca aggatcacca cctgctgacc tcggtggcgg
     4141 agaagtctgg cgatgaatcg gacgagtcgg actcagacac ttcggcggaa ccaaagcagg
     4201 agcgagactt tctaaccaat ccctactgga tcgaggaccc cgatgtacgc aagggcgagg
     4261 tcgattttct ctccagcacc gagatccagt tctggaagga tctcatcgac cagtatctct
     4321 atcccatcga caatgatccc gtggagcagg cccgcatagc caaagatctg aaggagctgc
     4381 gcgactcgtc ggtgttcgcg ttcttcatga tcaatgcact gttcgtgctg atcgtgttcc
     4441 tgctgcagct gaacaaggac aacatccacg tgaagtggcc cttcggagtg cggacgaaca
     4501 tcacctacga cgagtccacc caggaggtgc acatctccaa ggagtacctt cagctggagc
     4561 ccatcggatt ggtgttcgta ttcttctttg ctcttatttt gataatccag ttcacggcca
     4621 tgctgttcca tcgatttgga accatttcgc acatcctggc ctcgactgaa ctcaacttct
     4681 gcaagaagaa gtctgaggat ctgacacagg accaactaat agataagcat gctgtggaga
     4741 tcgtcaagaa cttgcagagg ctgcagggaa tcgatggcga ctacgacaat gactccggtt
     4801 ccgggccaga tcgcatagcc aggcggaaga ccattcaaaa tctggagaag gcgcgacagc
     4861 cacgtcgcca gatcggcacc ctggatgtgg cgttcaagaa gcgcttcctg aaactcaccg
     4921 ccgatgcgga gaacaatccg gccacgccca ttctcacccg ccgcctgaca atgcgggcgg
     4981 agaccatccg ggcactggag gtgcgtaaga attcggtgat ggccgaaagg cggaagtccg
     5041 cgatgcaaac tctgggagca aagaacgagt acggaatcac aactggagca ccgattaaca
     5101 acaatggagc tctgccgaat cagcgaagcg gacgtgtctc gaatgcaggc atcagcatca
     5161 aggatgtatt caatgtgaac ggtggtggtg ccgagcagat ttacggctcg aatgggggcg
     5221 gcactatcaa ccagggttac gagcatgtga tcgacgagga cggcgacggc aactccctgc
     5281 ggctgaccac caggaatccc catccccacc cgcaccacca ggtctcctgg ggccagaaca
     5341 ccaacggcgg cggcggtaat ggcacaggtc gcctgatggt gagcaagggc gaggaggata
     5401 acatggcctc tctcccagcg acacatgagt tacacatctt tggctccatc aacggtgtgg
     5461 actttgacat ggtgggtcag ggcaccggca atccaaatga tggttatgag gagttaaacc
     5521 tgaagtccac caagggtgac ctccagttct ccccctggat tctggtccct catatcgggt
     5581 atggcttcca tcagtacctg ccctaccctg acgggatgtc gcctttccag gccgccatgg
     5641 tagatggctc cggataccaa gtccatcgca caatgcagtt tgaagatggt gcctccctta
     5701 ctgttaacta ccgctacacc tacgagggaa gccacatcaa aggagaggcc caggtgaagg
     5761 ggactggttt ccctgctgac ggtcctgtga tgaccaactc gctgaccgct gcggactggt
     5821 gcaggtcgaa gaagacttac cccaacgaca aaaccatcat cagtaccttt aagtggagtt
     5881 acaccactgg aaatggcaag cgctaccgga gcactgcgcg gaccacctac acctttgcca
     5941 agccaatggc ggctaactat ctgaagaacc agccgatgta cgtgttccgt aagacggagc
     6001 tcaagcactc caagaccgag ctcaacttca aggagtggca aaaggccttt accgatgtga
     6061 tgggcatgga cgagctgtac aagtaatagt ctagaggatc tttgtgaagg aaccttactt
     6121 ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat
     6181 aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat
     6241 tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct
     6301 gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc
     6361 tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct
     6421 aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac
     6481 cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt tgatgtatag
     6541 tgccttgact agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     6601 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     6661 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     6721 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     6781 atcatgtctg gatccactag tgtcgacgat gtaggtcacg gtctcgaagc cgcggtgcgg
     6841 gtgccagggc gtgcccttgg gctccccggg cgcgtactcc acctcaccca tctggtccat
     6901 catgatgaac gggtcgaggt ggcggtagtt gatcccggcg aacgcgcggc gcaccgggaa
     6961 gccctcgccc tcgaaaccgc tgggcgcggt ggtcacggtg agcacgggac gtgcgacggc
     7021 gtcggcgggt gcggatacgc ggggcagcgt cagcgggttc tcgacggtca cggcgggcat
     7081 gtcgacacta gttctagcca gcttttgttc cctttagtga gggttaattt cgagcttggc
     7141 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     7201 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     7261 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     7321 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     7381 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     7441 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     7501 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     7561 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     7621 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     7681 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     7741 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     7801 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     7861 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     7921 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     7981 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     8041 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     8101 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     8161 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     8221 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     8281 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     8341 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     8401 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     8461 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     8521 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     8581 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     8641 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     8701 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     8761 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     8821 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     8881 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     8941 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     9001 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     9061 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     9121 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     9181 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     9241 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     9301 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     9361 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     9421 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     9481 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     9541 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     9601 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     9661 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     9721 acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc
     9781 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     9841 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
     9901 aaaacgacgg ccagtgaatt gtaatacgac tcactatagg gcgaattggg tacgtaccgg
     9961 gcccctagta tgtatgtaag ttaataaaac ccatttttgc ggaaagtaga taaaaaaaac
    10021 attttttttt tttactgcac tggatatcat tgaacttatc tgatcagttt taaatttact
    10081 tcgatccaag ggtatttgat gtaccaggtt ctttcgatta cctctcactc aaaatgacat
    10141 tccactcaaa gtcagcgctg tttgcctcct tctctgtcca cagaaatatc gccgtctctt
    10201 tcgccgctgc gtccgctatc tctttcgcca ccgtttgtag cgttacgtag cgtcaatgtc
    10261 cgccttcagt tgcattttgt cagcggtttc gtgacgaagc tccaagcggt ttacgccatc
    10321 aattaaacac aaagtgctgt gccaaaactc ctctcgcttc ttatttttgt ttgttttttg
    10381 agtgattggg gtggtgattg gttttgggtg ggtaagcagg ggaaagtgtg aaaaatcccg
    10441 gcaatgggcc aagaggatca ggagctatta attcgcggag gcagcaaaca cccatctgcc
    10501 gagcatctga acaatgtgag tagtacatgt gcatacatct taagttcact tgatctatag
    10561 gaactgcgat tgcaacatca aattgtctgc ggcgtgagaa ctgcgaccca caaaaatccc
    10621 aaaccgcaat tgcacaaaca aatagtgaca cgaaacagat tattctggta gctgttctcg
    10681 ctatataaga caatttttga gatcatatca tgatcaagac atctaaaggc attcattttc
    10741 gactatattc ttttttacaa aaaatataac aaccagatat tttaagctga tcctagatgc
    10801 acaaaaaata aataaaagta taaacctact tcgtaggata cttcggggta ctttttgttc
    10861 ggggttagat gagcataacg cttgtagttg atatttgaga tcccctatca ttgcagggtg
    10921 acagcggagc ggcttcgcag agctgcatta accagggctt cgggcaggcc aaaaactacg
    10981 gcacgctccg gccacccagt ccgccggagg actccggttc agggagcggc caactagccg
    11041 agaacctcac ctatgcctgg cacaatatgg acatctttgg ggcggtcaat cagccgggct
    11101 ccggatggcg gcagctggtc aaccggacac gcggactatt ctgcaacgag cgacacatac
    11161 cggcgcccag gaaacatttg ctcaagaacg gtgagtttct attcgcagtc ggctgatctg
    11221 tgtgaaatct taataaaggg tccaattacc aatttgaaac tcagtttgcg gcgtggccta
    11281 tccgggcgaa cttttggccg tgatgggcag ttccggtgcc ggaaagacga ccctgctgaa
    11341 tgcccttgcc tttcgatcgc cgcagggcat ccaagtatcg ccatccggga tgcgactgct
    11401 caatggccaa cctgtggacg ccaaggagat gcaggccagg tgcgcctatg tccagcagga
    11461 tgacctcttt atcggctccc taacggccag ggaacacctg attttccaag ccatggtgcg
    11521 gatgccacga catctgacct atcggcagcg agtggcccgc gtggatcagg tgatccagga
    11581 gctttcgctc agcaaatgtc agcacacgat catcggtgtg cccggcaggg tgaaaggtct
    11641 gtccggcgga gaaaggaagc gtctggcatt cgcctccgag gctctaaccg atccgccgct
    11701 tctgatctgc gatgagccca cctccggact ggactccttt accgcccaca gcgtcgtcca
    11761 ggtgctgaag aagctgtcgc agaagggcaa gaccgtcatc ctgaccattc atcagccgtc
    11821 ttccgagctg tttgagctct ttgacaagat ccttctgatg gccgagggca gggtagcttt
    11881 cttgggcact cccagcgaag ccgtcgactt cttttcctag tgagttcgat gtgtttatta
    11941 agggtatcta gtattacata acatctcaac tcctatccag cgtgggtgcc cagtgtccta
    12001 ccaactacaa tccggcggac ttttacgtac aggtgttggc cgttgtgccc ggacgggaga
    12061 tcgagtcccg tgatcggatc gccaagatat gcgacaattt tgccattagc aaagtagccc
    12121 gggatatgga gcagttgttg gccaccaaaa atctggagaa gccactggag cagccggaga
    12181 atgggtacac ctacaaggcc acctggttca tgcagttccg ggcggtcctg tggcgatcct
    12241 ggctgtcggt gctcaaggaa ccactcctcg taaaagtgcg acttattcag acaacggtga
    12301 gtggttccag tggaaacaaa tgatataacg cttacaattc ttggaaacaa attcgctaga
    12361 ttttagatag aattgcctga ttccacaccc ttcttagttt ttttcaatga gatgtatagt
    12421 ttatagtttt gcagaagata aataaatttc atttaactcg cgaatattaa tgagatgcga
    12481 gtaacatttt aatttgcaga tggttgccat cttgattggc ctcatctttt tgggccaaca
    12541 actcacgcaa gtgggtgtga tgaatatcaa cggagccatc ttcctcttcc tgaccaacat
    12601 gacctttcaa aacgtctttg ccacgataaa tgtaagtcat gtttagaata catttgcatt
    12661 tcaataattt actaactttc taatgaatcg attcgattta ggtgttcacc tcagagctgc
    12721 cagtttttat gagggaggcc cgaagtcgac tttatcgctg tgacacatac tttctgggca
    12781 aaacgattgc cgaattgccg ctttttctca cagtgccact ggtcttcacg gcgattgcct
    12841 atccgatgat cggactgcgg gccggagtgc tgcacttctt caactgcctg gcgctggtca
    12901 ctctggtggc caatgtgtca acgtccttcg gatatctaat atcctgcgcc agctcctcga
    12961 cctcgatggc gctgtctgtg ggtccgccgg ttatcatacc attcctgctc tttggcggct
    13021 tcttcttgaa ctcgggctcg gtgccagtat acctcaaatg gttgtcgtac ctctcatggt
    13081 tccgttacgc caacgagggt ctgctgatta accaatgggc ggacgtggag ccgggcgaaa
    13141 ttagctgcac atcgtcgaac accacgtgcc ccagttcggg caaggtcatc ctggagacgc
    13201 ttaacttctc cgccgccgat ctgccgctgg actacgtggg tctggccatt ctcatcgtga
    13261 gcttccgggt gctcgcatat ctggctctaa gacttcgggc ccgacgcaag gagtagccga
    13321 catatatccg aaataactgc ttgttttttt tttttaccat tattaccatc gtgtttactg
    13381 tttattgccc cctcaaaaag ctaatgtaat tatatttgtg ccaataaaaa caagatatga
    13441 cctatagaat acaagtattt ccccttcgaa catccccaca agtagacttt ggatttgtct
    13501 tctaaccaaa agacttacac acctgcatac cttacatcaa aaactcgttt atcgctacat
    13561 aaaacaccgg gatatatttt ttatatacat acttttcaaa tcgcgcgccc tcttcataat
    13621 tcacctccac cacaccacgt ttcgtagttg ctctttcgct gtctcccacc cgctctccgc
    13681 aacacattca ccttttgttc gacgaccttg gagcgactgt cgttagttcc gcgcgattcg
    13741 gttcgctcaa atggttccga gtggttcatt tcgtctcaat agaaattagt aataaatatt
    13801 tgtatgtaca atttatttgc tccaatatat ttgtatatat ttccctcaca gctatattta
    13861 ttctaattta atattatgac tttttaaggt aattttttgt gacctgttcg gagtgattag
    13921 cgttacaatt tgaactgaaa gtgacatcca gtgtttgttc cttgtgtaga tgcatctcaa
    13981 aaaaatggtg ggcataatag tgttgtttat atatatcaaa aataa
//
