LOCUS       sequence-274827-        4755 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express first domain (1-250) of TOG .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Ryan VH, Perdikari TM, Naik MT, Saueressig CF, Lins J, Dignon GL, 
            Mittal J, Hart AC, Fawzi NL
  TITLE     Tyrosine phosphorylation regulates hnRNPA2 granule protein 
            partitioning and reduces neurodegeneration.
  JOURNAL   EMBO J. 2021 Feb 1;40(3):e105001. doi: 10.15252/embj.2020105001. 
            Epub 2020 Dec 22.
  PUBMED    33349959
REFERENCE   2  (bases 1 to 4755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2021-02-1"; volume: "40(3):e105001. doi"; pages: " 
            10.15252/embj.2020105001. Epub 2020 Dec 22"
COMMENT     Sequence Label: TOG_D1
FEATURES             Location/Qualifiers
     source          1..4755
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     85..104
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        85..103
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    104..128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             189..206
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             207..227
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     terminator      994..1041
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(1264..1283)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     promoter        1350..1441
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1442..2251
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             2334..3416
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(2353..2372)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     rep_origin      3502..4090
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3991..4010
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4244..4261
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     4359..4376
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4360..4376
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 atacaaaata taatacaaac tataagatgt tatcagtatt tattatgcat ttagaataaa
       61 ttttgtgtcg cccttccgcg aaattaatac gactcactat aggggaattg tgagcggata
      121 acaattcccc tctagaaata attttgttta acttttgaag gagatatacc atgggctctg
      181 ataaaattca tcaccatcac catcacgaaa atctttattt tcaaggtcat atgggtgatg
      241 attcagaatg gttaaaactg cctgtcgatc aaaaatgcga gcacaaattg tggaaggccc
      301 gtctgtctgg ctatgaagag gcactgaaga ttttccaaaa gatcaaagac gagaaatccc
      361 cagagtggtc caaatttctg ggtttgatca agaaatttgt tacggacagc aatgccgttg
      421 tgcagttgaa aggcctggaa gcggcactgg tgtatgtgga gaacgcgcat gtggcgggca
      481 agaccacggg tgaagttgtt agcggtgtcg ttagcaaggt tttcaaccag ccgaaggcaa
      541 aggcaaaaga actgggtatt gagatttgtc tgatgtacat cgagattgag aaaggtgaag
      601 ctgtgcaaga agaactgctg aagggtctgg acaataagaa tccgaagatc attgtcgcgt
      661 gcatcgaaac cctgcgcaaa gcactgagcg agttcggcag caagattatc ctgctgaagc
      721 cgattatcaa agtgctgccg aagctgttcg agagccgcga gaaagctgtg cgcgatgaag
      781 cgaaattgat tgcggttgag atttaccgtt ggatccgtga cgccttgcgt ccgccgctgc
      841 agaacattaa cagcgtccag ctgaaagaac tggaagagga atgggtcaag ctgccgacct
      901 cggcgccgcg tccgactcgt tttctgcgta gccaacaaga attggaagcg aagctcgaac
      961 aacagcagag cgccggtggc taactcgagc cccctagcat aaccccttgg ggcctctaaa
     1021 cgggtcttga ggggtttttt gcccctgaga cgcgtcaatc gagttcgtac ctaagggcga
     1081 caccccctaa ttagcccggg cgaaaggccc agtctttcga ctgagccttt cgttttattt
     1141 gatgcctggc agttccctac tctcgcatgg ggagtcccca cactaccatc ggcgctacgg
     1201 cgtttcactt ctgagttcgg catggggtca ggtgggacca ccgcgctact gccgccaggc
     1261 aaacaagggg tgttatgagc catattcagg tataaatggg ctcgcgataa tgttcagaat
     1321 tggttaattg gttgtaacac tgacccctat ttgtttattt ttctaaatac attcaaatat
     1381 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagaa
     1441 tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga
     1501 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
     1561 cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
     1621 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgccacttcc
     1681 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc
     1741 cggaaaaaca gcgttccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
     1801 tgcgctggca gtgttcctgc gccggttgca ctcgattcct gtttgtaatt gtccttttaa
     1861 cagcgatcgc gtatttcgcc tcgctcaggc gcaatcacga atgaataacg gtttggttga
     1921 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
     1981 gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
     2041 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat
     2101 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc
     2161 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
     2221 atttcatttg atgctcgatg agtttttcta agcggcgcgc catcgaatgg cgcaaaacct
     2281 ttcgcggtat ggcatgatag cgcccggaag agagtcaatt cagggtggtg aatatgaaac
     2341 cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg
     2401 tggtgaacca ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg
     2461 cggagctgaa ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc
     2521 tgattggcgt tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga
     2581 ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg
     2641 gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga
     2701 tcattaacta tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg
     2761 ttccggcgtt atttcttgat gtctctgacc agacacccat caacagtatt attttctccc
     2821 atgaggacgg tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg
     2881 cgctgttagc gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata
     2941 aatatctcac tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca
     3001 tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc
     3061 tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc
     3121 gcgttggtgc ggatatctcg gtagtgggat acgacgatac cgaagatagc tcatgttata
     3181 tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc
     3241 gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg ccagtctcac
     3301 tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg
     3361 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgactca
     3421 tgaccaaaat cccttaacgt gagttacgcg cgcgtcgttc cactgagcgt cagaccccgt
     3481 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3541 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3601 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     3661 gccgtagtta gcccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3721 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3781 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3841 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3901 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3961 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     4021 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     4081 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     4141 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     4201 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     4261 ggaagcggaa ggcgagagta gggaactgcc aggcatcaaa ctaagcagaa ggcccctgac
     4321 ggatggcctt tttgcgtttc tacaaactct ttctgtgttg taaaacgacg gccagtctta
     4381 agctcgggcc ccctgggcgg ttctgataac gagtaatcgt taatccgcaa ataacgtaaa
     4441 aacccgcttc ggcgggtttt tttatggggg gagtttaggg aaagagcatt tgtcagaata
     4501 tttaagggcg cctgtcactt tgcttgatat atgagaatta tttaacctta taaatgagaa
     4561 aaaagcaacg cactttaaat aagatacgtt gctttttcga ttgatgaaca cctataatta
     4621 aactattcat ctattattta tgattttttg tatatacaat atttctagtt tgttaaagag
     4681 aattaagaaa ataaatctcg aaaataataa agggaaaatc agtttttgat atcaaaatta
     4741 tacatgtcaa cgata
//
