LOCUS       sequence-274830-        4506 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express the LC of HRP-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Ryan VH, Perdikari TM, Naik MT, Saueressig CF, Lins J, Dignon GL, 
            Mittal J, Hart AC, Fawzi NL
  TITLE     Tyrosine phosphorylation regulates hnRNPA2 granule protein 
            partitioning and reduces neurodegeneration.
  JOURNAL   EMBO J. 2021 Feb 1;40(3):e105001. doi: 10.15252/embj.2020105001. 
            Epub 2020 Dec 22.
  PUBMED    33349959
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2021-02-1"; volume: "40(3):e105001. doi"; pages: " 
            10.15252/embj.2020105001. Epub 2020 Dec 22"
COMMENT     Sequence Label: HRP-1LC
FEATURES             Location/Qualifiers
     source          1..4506
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     185..204
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        185..203
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    204..228
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             289..306
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             307..327
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     terminator      845..892
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(1115..1134)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     promoter        1201..1292
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1293..2102
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             2185..3267
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(2204..2223)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     rep_origin      3353..3941
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3842..3861
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4095..4112
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     4210..4227
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4211..4227
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acaatatttc tagtttgtta aagagaatta agaaaataaa tctcgaaaat aataaaggga
       61 aaatcagttt ttgatatcaa aattatacat gtcaacgata atacaaaata taatacaaac
      121 tataagatgt tatcagtatt tattatgcat ttagaataaa ttttgtgtcg cccttccgcg
      181 aaattaatac gactcactat aggggaattg tgagcggata acaattcccc tctagaaata
      241 attttgttta acttttgaag gagatatacc atgggcagcg acaaaattca ccatcatcac
      301 catcacgaga atctgtactt ccaaggtcat atgggccatc gctgcgacgt ccgtaagggt
      361 ttgagcaaag acgaaatgtc caaggcacaa atgaaccgtg acagagaaac gcgcggtggt
      421 cgtagccgtg atggtcagcg tggcggctac aacggtggtg gcggtggtgg tggaggatgg
      481 ggcggtccag cacagcgtgg tggccctggt gcgtacggcg gtcctggtgg tgggggtcag
      541 ggtggctatg gcggcgatta tggtggcggc tggggtcagc aaggtggtgg tggccagggt
      601 ggttggggtg gtccgcagca gcaacagggt ggtggtgggt ggggtcaaca aggcggtggc
      661 ggtcaaggtg gatggggtgg cccacagcaa caacagcaag gcggttgggg aggtccgcaa
      721 caaggtggcg gcggtggagg ttggggcggt cagggtcagc agcagggtgg ctggggtggt
      781 caatctggtg cgcaacagtg ggctcatgcg cagggcggca atcgtaacta ttaactcgag
      841 ccccctagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgcccctgag
      901 acgcgtcaat cgagttcgta cctaagggcg acacccccta attagcccgg gcgaaaggcc
      961 cagtctttcg actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcatg
     1021 gggagtcccc acactaccat cggcgctacg gcgtttcact tctgagttcg gcatggggtc
     1081 aggtgggacc accgcgctac tgccgccagg caaacaaggg gtgttatgag ccatattcag
     1141 gtataaatgg gctcgcgata atgttcagaa ttggttaatt ggttgtaaca ctgaccccta
     1201 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     1261 aaatgcttca ataatattga aaaaggaaga atatgagcca tattcaacgg gaaacgtcga
     1321 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
     1381 atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt
     1441 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
     1501 taaactggct gacggaattt atgccacttc cgaccatcaa gcattttatc cgtactcctg
     1561 atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcgttccag gtattagaag
     1621 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc
     1681 actcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgc ctcgctcagg
     1741 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg
     1801 gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt
     1861 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa
     1921 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc
     1981 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg
     2041 gtattgataa tcctgatatg aataaattgc aatttcattt gatgctcgat gagtttttct
     2101 aagcggcgcg ccatcgaatg gcgcaaaacc tttcgcggta tggcatgata gcgcccggaa
     2161 gagagtcaat tcagggtggt gaatatgaaa ccagtaacgt tatacgatgt cgcagagtat
     2221 gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg
     2281 aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg
     2341 gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc
     2401 ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc
     2461 agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac
     2521 aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat
     2581 gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac
     2641 cagacaccca tcaacagtat tattttctcc catgaggacg gtacgcgact gggcgtggag
     2701 catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc
     2761 tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg
     2821 atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg
     2881 ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc
     2941 gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga
     3001 tacgacgata ccgaagatag ctcatgttat atcccgccgt taaccaccat caaacaggat
     3061 tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg
     3121 gtgaagggca atcagctgtt gccagtctca ctggtgaaaa gaaaaaccac cctggcgccc
     3181 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     3241 gtttcccgac tggaaagcgg gcagtgactc atgaccaaaa tcccttaacg tgagttacgc
     3301 gcgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     3361 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     3421 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     3481 cgcagatacc aaatactgtt cttctagtgt agccgtagtt agcccaccac ttcaagaact
     3541 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3601 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3661 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     3721 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     3781 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3841 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3901 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3961 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     4021 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     4081 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga aggcgagagt agggaactgc
     4141 caggcatcaa actaagcaga aggcccctga cggatggcct ttttgcgttt ctacaaactc
     4201 tttctgtgtt gtaaaacgac ggccagtctt aagctcgggc cccctgggcg gttctgataa
     4261 cgagtaatcg ttaatccgca aataacgtaa aaacccgctt cggcgggttt ttttatgggg
     4321 ggagtttagg gaaagagcat ttgtcagaat atttaagggc gcctgtcact ttgcttgata
     4381 tatgagaatt atttaacctt ataaatgaga aaaaagcaac gcactttaaa taagatacgt
     4441 tgctttttcg attgatgaac acctataatt aaactattca tctattattt atgatttttt
     4501 gtatat
//
