LOCUS       sequence-274835-       12640 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for human ACE2 with an N-terminal 
            (extracellular) c-myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12640)
  AUTHORS   Chan KK, Dorosky D, Sharma P, Abbasi SA, Dye JM, Kranz DM, Herbert 
            AS, Procko E
  TITLE     Engineering human ACE2 to optimize binding to the spike protein of 
            SARS coronavirus 2.
  JOURNAL   Science. 2020 Sep 4;369(6508):1261-1265. doi: 
            10.1126/science.abc0870. Epub 2020 Aug 4.
  PUBMED    32753553
REFERENCE   2  (bases 1 to 12640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abc0870"; journalName: "Science"; date: "2020-09-4-
            4"; volume: "369"; issue: "6508"; pages: "1261-1265"
COMMENT     Sequence Label: pCEP4-myc-ACE2
FEATURES             Location/Qualifiers
     source          1..12640
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             61..90
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    2495..2629
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2519..2538)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2573..2592
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2772..2794)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3354..5143
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(5445..7370)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(7805..7823)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7891..7995
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7996..8064,8065..8856)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8214..8233)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9027..9615
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9516..9535
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        10046..10191
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             10234..11271
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(11269..11288)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11313..11361
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        12015..12394
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12395..12598
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12549..12569
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     12595..12619
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gctagcgcca ccatgaagac catcatcgcc ctgagctaca tcttctgcct ggtgttcgcc
       61 gagcaaaagc tgatctcaga ggaggacctg ggcagccctg gcggtgcctc caccattgag
      121 gaacaggcca agacattttt ggacaagttt aaccacgaag ccgaagacct gttctatcaa
      181 agttcacttg cttcttggaa ttataacacc aatattactg aagagaatgt ccaaaacatg
      241 aataatgctg gggacaaatg gtctgccttt ttaaaggaac agtccacact tgcccaaatg
      301 tatccactac aagaaattca gaatctcaca gtcaagcttc agctgcaggc tcttcagcaa
      361 aatgggtctt cagtgctctc agaagacaag agcaaacggt tgaacacaat tctaaataca
      421 atgagcacca tctacagtac tggaaaagtt tgtaacccag ataatccaca agaatgctta
      481 ttacttgaac caggtttgaa tgaaataatg gcaaacagtt tagactacaa tgagaggctc
      541 tgggcttggg aaagctggag atctgaggtc ggcaagcagc tgaggccatt atatgaagag
      601 tatgtggtct tgaaaaatga gatggcaaga gcaaatcatt atgaggacta tggggattat
      661 tggagaggag actatgaagt aaatggggta gatggctatg actacagccg cggccagttg
      721 attgaagatg tggaacatac ctttgaagag attaaaccat tatatgaaca tcttcatgcc
      781 tatgtgaggg caaagttgat gaatgcctat ccttcctata tcagtccaat tggatgcctc
      841 cctgctcatt tgcttggtga tatgtggggt agattttgga caaatctgta ctctttgaca
      901 gttccctttg gacagaaacc aaacatagat gttactgatg caatggtgga ccaggcctgg
      961 gatgcacaga gaatattcaa ggaggccgag aagttctttg tatctgttgg tcttcctaat
     1021 atgactcaag gattctggga aaattccatg ctaacggacc caggaaatgt tcagaaagca
     1081 gtctgccatc ccacagcttg ggacctgggg aagggcgact tcaggatcct tatgtgcaca
     1141 aaggtgacaa tggacgactt cctgacagct catcatgaga tggggcatat ccagtatgat
     1201 atggcatatg ctgcacaacc ttttctgcta agaaatggag ctaatgaagg attccatgaa
     1261 gctgttgggg aaatcatgtc actttctgca gccacaccta agcatttaaa atccattggt
     1321 cttctgtcac ccgattttca agaagacaat gaaacagaaa taaacttcct gctcaaacaa
     1381 gcactcacga ttgttgggac tctgccattt acttacatgt tagagaagtg gaggtggatg
     1441 gtctttaaag gggaaattcc caaagaccag tggatgaaaa agtggtggga gatgaagcga
     1501 gagatagttg gggtggtgga acctgtgccc catgatgaaa catactgtga ccccgcatct
     1561 ctgttccatg tttctaatga ttactcattc attcgatatt acacaaggac cctttaccaa
     1621 ttccagtttc aagaagcact ttgtcaagca gctaaacatg aaggccctct gcacaaatgt
     1681 gacatctcaa actctacaga agctggacag aaactgttca atatgctgag gcttggaaaa
     1741 tcagaaccct ggaccctagc attggaaaat gttgtaggag caaagaacat gaatgtaagg
     1801 ccactgctca actactttga gcccttattt acctggctga aagaccagaa caagaattct
     1861 tttgtgggat ggagtaccga ctggagtcca tatgcagacc aaagcatcaa agtgaggata
     1921 agcctaaaat cagctcttgg agataaagca tatgaatgga acgacaatga aatgtacctg
     1981 ttccgatcat ctgttgcata tgctatgagg cagtactttt taaaagtaaa aaatcagatg
     2041 attctttttg gggaggagga tgtgcgagtg gctaatttga aaccaagaat ctcctttaat
     2101 ttctttgtca ctgcacctaa aaatgtgtct gatatcattc ctagaactga agttgaaaag
     2161 gccatcagga tgtcccggag ccgtatcaat gatgctttcc gtctgaatga caacagccta
     2221 gagtttctgg ggatacagcc aacacttgga cctcctaacc agccccctgt ttccatatgg
     2281 ctgattgttt ttggagttgt gatgggagtg atagtggttg gcattgtcat cctgatcttc
     2341 actgggatca gagatcggaa gaagaaaaat aaagcaagaa gtggagaaaa tccttatgcc
     2401 tccatcgata ttagcaaagg agaaaataat ccaggattcc aaaacactga tgatgttcag
     2461 acctcctttt agtaactcga ggccggcaag gccggatcca gacatgataa gatacattga
     2521 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     2581 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     2641 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
     2701 aaacctctac aaatgtggta tggctgatta tgatccggct gcctcgcgcg tttcggtgat
     2761 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     2821 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     2881 gcagccatga ggtcgactct agaggatcga tccccgcccc ggacgaacta aacctgacta
     2941 cgacatctct gccccttctt cgcggggcag tgcatgtaat cccttcagtt ggttggtaca
     3001 acttgccaac tgggccctgt tccacatgtg acacgggggg ggaccaaaca caaaggggtt
     3061 ctctgactgt agttgacatc cttataaatg gatgtgcaca tttgccaaca ctgagtggct
     3121 ttcatcctgg agcagacttt gcagtctgtg gactgcaaca caacattgcc tttatgtgta
     3181 actcttggct gaagctctta caccaatgct gggggacatg tacctcccag gggcccagga
     3241 agactacggg aggctacacc aacgtcaatc agaggggcct gtgtagctac cgataagcgg
     3301 accctcaaga gggcattagc aatagtgttt ataaggcccc cttgttaacc ctaaacgggt
     3361 agcatatgct tcccgggtag tagtatatac tatccagact aaccctaatt caatagcata
     3421 tgttacccaa cgggaagcat atgctatcga attagggtta gtaaaagggt cctaaggaac
     3481 agcgatatct cccaccccat gagctgtcac ggttttattt acatggggtc aggattccac
     3541 gagggtagtg aaccatttta gtcacaaggg cagtggctga agatcaagga gcgggcagtg
     3601 aactctcctg aatcttcgcc tgcttcttca ttctccttcg tttagctaat agaataactg
     3661 ctgagttgtg aacagtaagg tgtatgtgag gtgctcgaaa acaaggtttc aggtgacgcc
     3721 cccagaataa aatttggacg gggggttcag tggtggcatt gtgctatgac accaatataa
     3781 ccctcacaaa ccccttgggc aataaatact agtgtaggaa tgaaacattc tgaatatctt
     3841 taacaataga aatccatggg gtggggacaa gccgtaaaga ctggatgtcc atctcacacg
     3901 aatttatggc tatgggcaac acataatcct agtgcaatat gatactgggg ttattaagat
     3961 gtgtcccagg cagggaccaa gacaggtgaa ccatgttgtt acactctatt tgtaacaagg
     4021 ggaaagagag tggacgccga cagcagcgga ctccactggt tgtctctaac acccccgaaa
     4081 attaaacggg gctccacgcc aatggggccc ataaacaaag acaagtggcc actctttttt
     4141 ttgaaattgt ggagtggggg cacgcgtcag cccccacacg ccgccctgcg gttttggact
     4201 gtaaaataag ggtgtaataa cttggctgat tgtaaccccg ctaaccactg cggtcaaacc
     4261 acttgcccac aaaaccacta atggcacccc ggggaatacc tgcataagta ggtgggcggg
     4321 ccaagatagg ggcgcgattg ctgcgatctg gaggacaaat tacacacact tgcgcctgag
     4381 cgccaagcac agggttgttg gtcctcatat tcacgaggtc gctgagagca cggtgggcta
     4441 atgttgccat gggtagcata tactacccaa atatctggat agcatatgct atcctaatct
     4501 atatctgggt agcataggct atcctaatct atatctgggt agcatatgct atcctaatct
     4561 atatctgggt agtatatgct atcctaattt atatctgggt agcataggct atcctaatct
     4621 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct
     4681 gtatccgggt agcatatgct atcctaatag agattagggt agtatatgct atcctaattt
     4741 atatctgggt agcatatact acccaaatat ctggatagca tatgctatcc taatctatat
     4801 ctgggtagca tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat
     4861 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat
     4921 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     4981 ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc tcatgcatat
     5041 acagtcagca tatgataccc agtagtagag tgggagtgct atcctttgca tatgccgcca
     5101 cctcccaagg gggcgtgaat tttcgctgct tgtccttttc ctgctggttg ctcccattct
     5161 taggtgaatt taaggaggcc aggctaaagc cgtcgcatgt ctgattgctc accaggtaaa
     5221 tgtcgctaat gttttccaac gcgagaaggt gttgagcgcg gagctgagtg acgtgacaac
     5281 atgggtatgc ccaattgccc catgttggga ggacgaaaat ggtgacaaga cagatggcca
     5341 gaaatacacc aacagcacgc atgatgtcta ctggggattt attctttagt gcgggggaat
     5401 acacggcttt taatacgatt gagggcgtct cctaacaagt tacatcactc ctgcccttcc
     5461 tcaccctcat ctccatcacc tccttcatct ccgtcatctc cgtcatcacc ctccgcggca
     5521 gccccttcca ccataggtgg aaaccaggga ggcaaatcta ctccatcgtc aaagctgcac
     5581 acagtcaccc tgatattgca ggtaggagcg ggctttgtca taacaaggtc cttaatcgca
     5641 tccttcaaaa cctcagcaaa tatatgagtt tgtaaaaaga ccatgaaata acagacaatg
     5701 gactccctta gcgggccagg ttgtgggccg ggtccagggg ccattccaaa ggggagacga
     5761 ctcaatggtg taagacgaca ttgtggaata gcaagggcag ttcctcgcct taggttgtaa
     5821 agggaggtct tactacctcc atatacgaac acaccggcga cccaagttcc ttcgtcggta
     5881 gtcctttcta cgtgactcct agccaggaga gctcttaaac cttctgcaat gttctcaaat
     5941 ttcgggttgg aacctccttg accacgatgc tttccaaacc accctccttt tttgcgcctg
     6001 cctccatcac cctgaccccg gggtccagtg cttgggcctt ctcctgggtc atctgcgggg
     6061 ccctgctcta tcgctcccgg gggcacgtca ggctcaccat ctgggccacc ttcttggtgg
     6121 tattcaaaat aatcggcttc ccctacaggg tggaaaaatg gccttctacc tggagggggc
     6181 ctgcgcggtg gagacccgga tgatgatgac tgactactgg gactcctggg cctcttttct
     6241 ccacgtccac gacctctccc cctggctctt tcacgacttc cccccctggc tctttcacgt
     6301 cctctacccc ggcggcctcc actacctcct cgaccccggc ctccactacc tcctcgaccc
     6361 cggcctccac tgcctcctcg accccggcct ccacctcctg ctcctgcccc tcctgctcct
     6421 gcccctcctc ctgctcctgc ccctcctgcc cctcctgctc ctgcccctcc tgcccctcct
     6481 gctcctgccc ctcctgcccc tcctgctcct gcccctcctg cccctcctcc tgctcctgcc
     6541 cctcctgccc ctcctcctgc tcctgcccct cctgcccctc ctgctcctgc ccctcctgcc
     6601 cctcctgctc ctgcccctcc tgcccctcct gctcctgccc ctcctgctcc tgcccctcct
     6661 gctcctgccc ctcctgctcc tgcccctcct gcccctcctg cccctcctcc tgctcctgcc
     6721 cctcctgctc ctgcccctcc tgcccctcct gcccctcctg ctcctgcccc tcctcctgct
     6781 cctgcccctc ctgcccctcc tgcccctcct cctgctcctg cccctcctgc ccctcctcct
     6841 gctcctgccc ctcctcctgc tcctgcccct cctgcccctc ctgcccctcc tcctgctcct
     6901 gcccctcctg cccctcctcc tgctcctgcc cctcctcctg ctcctgcccc tcctgcccct
     6961 cctgcccctc ctcctgctcc tgcccctcct cctgctcctg cccctcctgc ccctcctgcc
     7021 cctcctgccc ctcctcctgc tcctgcccct cctcctgctc ctgcccctcc tgctcctgcc
     7081 cctcccgctc ctgctcctgc tcctgttcca ccgtgggtcc ctttgcagcc aatgcaactt
     7141 ggacgttttt ggggtctccg gacaccatct ctatgtcttg gccctgatcc tgagccgccc
     7201 ggggctcctg gtcttccgcc tcctcgtcct cgtcctcttc cccgtcctcg tccatggtta
     7261 tcaccccctc ttctttgagg tccactgccg ccggagcctt ctggtccaga tgtgtctccc
     7321 ttctctccta ggccatttcc aggtcctgta cctggcccct cgtcagacat gattcacact
     7381 aaaagagatc aatagacatc tttattagac gacgctcagt gaatacaggg agtgcagact
     7441 cctgccccct ccaacagccc ccccaccctc atccccttca tggtcgctgt cagacagatc
     7501 caggtctgaa aattccccat cctccgaacc atcctcgtcc tcatcaccaa ttactcgcag
     7561 cccggaaaac tcccgctgaa catcctcaag atttgcgtcc tgagcctcaa gccaggcctc
     7621 aaattcctcg tccccctttt tgctggacgg tagggatggg gattctcggg acccctcctc
     7681 ttcctcttca aggtcaccag acagagatgc tactggggca acggaagaaa agctgggtgc
     7741 ggcctgtgag gatcagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga
     7801 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     7861 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     7921 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     7981 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     8041 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     8101 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     8161 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     8221 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
     8281 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     8341 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     8401 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     8461 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     8521 cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa
     8581 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     8641 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     8701 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     8761 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     8821 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     8881 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     8941 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     9001 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     9061 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     9121 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     9181 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     9241 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     9301 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     9361 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     9421 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     9481 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     9541 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     9601 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     9661 ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg
     9721 ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc
     9781 gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccc cgagatgcgc cgcgtgcggc
     9841 tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag
     9901 ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc
     9961 gccctgcttc atccccgtgg cccgttgctc gcgtttgctg gcggtgtccc cggaagaaat
    10021 atatttgcat gtctttagtt ctatgatgac acaaaccccg cccagcgtct tgtcattggc
    10081 gaattcgaac acgcagatgc agtcggggcg gcgcggtccg aggtccactt cgcatattaa
    10141 ggtgacgcgt gtggcctcga acaccgagcg accctgcagc gacccgctta acagcgtcaa
    10201 cagcgtgccg cagatcccgg ggggcaatga gatatgaaaa agcctgaact caccgcgacg
    10261 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg
    10321 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg
    10381 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg
    10441 gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat
    10501 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc
    10561 gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag
    10621 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat
    10681 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc
    10741 gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc
    10801 gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc
    10861 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc
    10921 gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc
    10981 gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt
    11041 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg
    11101 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc
    11161 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga
    11221 aaccgacgcc ccagcactcg tccggatcgg gagatggggg aggctaactg aaacacggaa
    11281 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
    11341 cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg
    11401 ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc
    11461 accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg
    11521 ccatagccac tggccccgtg ggttagggac ggggtccccc atggggaatg gtttatggtt
    11581 cgtgggggtt attattttgg gcgttgcgtg gggtcaggtc cacgactgga ctgagcagac
    11641 agacccatgg tttttggatg gcctgggcat ggaccgcatg tactggcgcg acacgaacac
    11701 cgggcgtctg tggctgccaa acacccccga cccccaaaaa ccaccgcgcg gatttctggc
    11761 gtgccaagct agtcgaccaa ttctcatgtt tgacagctta tcatcgcaga tccgggcaac
    11821 gttgttgcca ttgctgcagg cgcagaactg gtaggtatgg aagatctata cattgaatca
    11881 atattggcaa ttagccatat tagtcattgg ttatatagca taaatcaata ttggctattg
    11941 gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct catgtccaat
    12001 atgaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
    12061 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
    12121 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
    12181 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
    12241 cttggcagta catcaagtgt atcatatgcc aagtccgccc cctattgacg tcaatgacgg
    12301 taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc ctacttggca
    12361 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacaccaa
    12421 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
    12481 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta ataaccccgc
    12541 cccgttgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
    12601 tttagtgaac cgtcagatct ctagaagctg ggtaccagct
//
