LOCUS       sequence-274853-        4161 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express prion like domain of hnRNPF .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Ryan VH, Perdikari TM, Naik MT, Saueressig CF, Lins J, Dignon GL, 
            Mittal J, Hart AC, Fawzi NL
  TITLE     Tyrosine phosphorylation regulates hnRNPA2 granule protein 
            partitioning and reduces neurodegeneration.
  JOURNAL   EMBO J. 2021 Feb 1;40(3):e105001. doi: 10.15252/embj.2020105001. 
            Epub 2020 Dec 22.
  PUBMED    33349959
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2021-02-1"; volume: "40(3):e105001. doi"; pages: " 
            10.15252/embj.2020105001. Epub 2020 Dec 22"
COMMENT     Sequence Label: hnRNPF_PLD
FEATURES             Location/Qualifiers
     source          1..4161
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     56..75
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        56..74
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    75..99
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             160..177
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             178..198
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     terminator      371..418
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(641..660)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     promoter        727..818
                     /gene="bla"
                     /label=AmpR promoter
     CDS             819..1628
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             1711..2793
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(1730..1749)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     rep_origin      2879..3467
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3368..3387
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3621..3638
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     3736..3753
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3737..3753
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ttatcagtat ttattatgca tttagaataa attttgtgtc gcccttccgc gaaattaata
       61 cgactcacta taggggaatt gtgagcggat aacaattccc ctctagaaat aattttgttt
      121 aacttttgaa ggagatatac catgggcagc gacaaaattc accatcatca ccatcacgag
      181 aatctgtact tccaaggtca tatgacgggc gcaagcaacg gtgcgtactc cagccaagtt
      241 atgcaaggca tgggtgtgag cgcggcacag gcgacgtaca gcggcctgga gagccagtcc
      301 gttagcggct gctatggtgc gggctatagc ggtcagaaca gcatgggcgg ctatgactga
      361 ctcgagcccc ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgcc
      421 cctgagacgc gtcaatcgag ttcgtaccta agggcgacac cccctaatta gcccgggcga
      481 aaggcccagt ctttcgactg agcctttcgt tttatttgat gcctggcagt tccctactct
      541 cgcatgggga gtccccacac taccatcggc gctacggcgt ttcacttctg agttcggcat
      601 ggggtcaggt gggaccaccg cgctactgcc gccaggcaaa caaggggtgt tatgagccat
      661 attcaggtat aaatgggctc gcgataatgt tcagaattgg ttaattggtt gtaacactga
      721 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
      781 cctgataaat gcttcaataa tattgaaaaa ggaagaatat gagccatatt caacgggaaa
      841 cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc
      901 gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc
      961 cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg
     1021 tcagactaaa ctggctgacg gaatttatgc cacttccgac catcaagcat tttatccgta
     1081 ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagcg ttccaggtat
     1141 tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc
     1201 ggttgcactc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgcctcg
     1261 ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc
     1321 gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac
     1381 cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga
     1441 aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg
     1501 ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa
     1561 aatatggtat tgataatcct gatatgaata aattgcaatt tcatttgatg ctcgatgagt
     1621 ttttctaagc ggcgcgccat cgaatggcgc aaaacctttc gcggtatggc atgatagcgc
     1681 ccggaagaga gtcaattcag ggtggtgaat atgaaaccag taacgttata cgatgtcgca
     1741 gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt
     1801 tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac
     1861 cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt
     1921 ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg
     1981 ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg
     2041 gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac
     2101 caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc
     2161 tctgaccaga cacccatcaa cagtattatt ttctcccatg aggacggtac gcgactgggc
     2221 gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt
     2281 tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt
     2341 cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg
     2401 caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg
     2461 ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta
     2521 gtgggatacg acgataccga agatagctca tgttatatcc cgccgttaac caccatcaaa
     2581 caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc
     2641 caggcggtga agggcaatca gctgttgcca gtctcactgg tgaaaagaaa aaccaccctg
     2701 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     2761 cgacaggttt cccgactgga aagcgggcag tgactcatga ccaaaatccc ttaacgtgag
     2821 ttacgcgcgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     2881 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     2941 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     3001 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttagcc caccacttca
     3061 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     3121 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     3181 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     3241 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     3301 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     3361 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     3421 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     3481 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     3541 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     3601 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaaggc gagagtaggg
     3661 aactgccagg catcaaacta agcagaaggc ccctgacgga tggccttttt gcgtttctac
     3721 aaactctttc tgtgttgtaa aacgacggcc agtcttaagc tcgggccccc tgggcggttc
     3781 tgataacgag taatcgttaa tccgcaaata acgtaaaaac ccgcttcggc gggttttttt
     3841 atggggggag tttagggaaa gagcatttgt cagaatattt aagggcgcct gtcactttgc
     3901 ttgatatatg agaattattt aaccttataa atgagaaaaa agcaacgcac tttaaataag
     3961 atacgttgct ttttcgattg atgaacacct ataattaaac tattcatcta ttatttatga
     4021 ttttttgtat atacaatatt tctagtttgt taaagagaat taagaaaata aatctcgaaa
     4081 ataataaagg gaaaatcagt ttttgatatc aaaattatac atgtcaacga taatacaaaa
     4141 tataatacaa actataagat g
//
