LOCUS       sequence-274857-        8902 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses OsTIR1 via the ADH1 promoter in yeast. The cassette is 
            integrated in the HO locus. Assembly and characterization of the 
            auxin-inducible degradation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8902)
  AUTHORS   Papagiannakis A, Niebel B, Wit EC, Heinemann M
  TITLE     Autonomous Metabolic Oscillations Robustly Gate the Early and Late 
            Cell Cycle.
  JOURNAL   Mol Cell. 2017 Jan 19;65(2):285-295. doi: 
            10.1016/j.molcel.2016.11.018. Epub 2016 Dec 15.
  PUBMED    27989441
REFERENCE   2  (bases 1 to 8902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2016.11.018"; journalName: "Mol Cell"; date: 
            "2017-01-19- 19"; volume: "65"; issue: "2"; pages: "285-295"
COMMENT     Sequence Label: pOsTIR1w/oGFP
FEATURES             Location/Qualifiers
     source          1..8902
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             416..2140
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     CDS             2168..2197
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2204..2233
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2240..2269
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      2419..2584
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2474..2498)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            2632..3988
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        2632..2975
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             2976..3785
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3043..3062)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      3791..3988
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(4596..4615)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4597..4615)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4622..4639)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4622..4638)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4631..4653)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4938..4957)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5070..5091
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5245..5264)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5364..5386
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5424..5442)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5510..5614
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5615..5683,5684..6475)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5833..5852)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6646..7234
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7135..7154
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7388..7405
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7522..7543
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    7598..7614
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7603..7625
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7622..7638
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7622..7638
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        join(8898..8902,1..397)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
ORIGIN
        1 caacttcttt tctttttttt tcttttctct ctcccccgtt gttgtctcac catatccgca
       61 atgacaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga cagcaccaac
      121 agatgtcgtt gttccagagc tgatgagggg tatctcgaag cacacgaaac tttttccttc
      181 cttcattcac gcacactact ctctaatgag caacggtata cggccttcct tccagttact
      241 tgaatttgaa ataaaaaaaa gtttgctgtc ttgctatcaa gtataaatag acctgcaatt
      301 attaatcttt tgtttcctcg tcattgttct cgttcccttt cttccttgtt tctttttctg
      361 cacaatattt caagctatac caagcataca atcaactatc tcatatacaa ctagaatgac
      421 gtacttcccg gaggaggtgg tggagcacat cttcagcttc ctgccggcgc agcgcgaccg
      481 caacacggtc tcgctcgtct gcaaggtgtg gtacgagatc gagaggctga gccgccgcgg
      541 cgtcttcgtg ggcaactgct acgccgtgcg cgccggccgc gtcgccgcgc ggttccccaa
      601 cgtgcgggcg ctcacggtga aggggaagcc ccacttcgcc gacttcaacc tcgtgccccc
      661 cgactggggc ggctacgcgg ggccgtggat cgaggcggcc gcgaggggat gccacggcct
      721 ggaggagctc aggatgaagc ggatggtggt gtccgacgag agcctcgagc tgctggctcg
      781 ctcgttcccg cggttcaggg ctcttgttct tatcagctgc gaggggttca gcactgacgg
      841 gctagccgcc gtcgcgagcc attgcaagct tctgagggag ttggatttgc aggaaaatga
      901 agtggaggat cgagggccta ggtggctttc ctgcttccct gattcctgca catcacttgt
      961 ctcattgaat tttgcctgca tcaaagggga ggttaatgct ggttcactgg agagacttgt
     1021 tagcaggtcc ccaaacctgc ggagtttgag gctgaatcga tctgtatcgg tagatacact
     1081 tgcaaagata ctactgcgta cccctaactt ggaggatttg gggacaggga atttgacaga
     1141 tgacttccaa actgagtcct actttaagct taccagtgct ctggagaaat gcaagatgtt
     1201 gaggagtttg tctggattct gggatgcttc tcctgtttgc ctgtcattta tctaccccct
     1261 gtgtgctcaa ctgacaggat tgaacttgag ctatgcaccc acacttgatg cttctgacct
     1321 tacaaaaatg attagccgct gtgtgaagct ccaacgcctt tgggtactgg attgtatctc
     1381 ggacaaaggc ttgcaagtgg tggcctccag ttgcaaagac ttgcaagaac tcagggtatt
     1441 tccatcagat ttctacgtag ctggttattc tgcagtgaca gaggagggac ttgttgcagt
     1501 atccttgggc tgtccaaaac tgaactcact actgtacttc tgtcaccaaa tgactaatgc
     1561 tgcactagtt actgtcgcca agaactgtcc aaatttcaca cgattcagac tttgtattct
     1621 tgagccaggg aagcctgatg ttgtgacaag ccaaccatta gatgaaggct ttggagctat
     1681 tgttcgtgag tgcaagggat tacaacgttt gtcaatatct ggtcttctca cagacaaagt
     1741 tttcatgtat attgggaaat atgcaaaaca acttgagatg ctttctatag catttgctgg
     1801 tgacagtgat aagggtatga tgcatgttat gaatggatgc aagaatttaa ggaaactgga
     1861 gataagagat agcccgtttg gtgatgctgc actcttgggg aattttgcta ggtacgagac
     1921 aatgcgatcc ctttggatgt catcttgcaa tgtcacgtta aaggggtgcc aagtccttgc
     1981 gtcaaagatg ccgatgctca atgttgaggt cataaatgag cgggatggta gcaatgaaat
     2041 ggaggaaaac catggagatc tgcctaaagt ggagaaatta tatgtgtacc gcacaactgc
     2101 tggggcgagg gatgatgcac caaattttgt taaaatccta gtcgactccg gttctgctgc
     2161 tagtggtgaa caaaagttga tttctgaaga agatttgaac ggtgaacaaa agctaatctc
     2221 cgaggaagac ttgaacggtg aacaaaaatt aatctcagaa gaagacttga acggatccac
     2281 tagctaaaac aattcttcgc cagaggtttg gtcaagtctc caatcaaggt tgtcggcttg
     2341 tctaccttgc cagaaattta cgaaaagatg gaaaagggtc aaatcgttgg tagatacgtt
     2401 gttgacactt ctaaataagc gaatttctta tgatttatga tttttattat taaataagtt
     2461 ataaaaaaaa taagtgtata caaattttaa agtgactctt aggttttaaa acgaaaattc
     2521 ttattcttga gtaactcttt cctgtaggtc aggttgcttt ctcaggtata gcatgaggtc
     2581 gctcgtagat ctgtttagct tgcctcgtcc ccgccgggtc acccggccag cgacatggag
     2641 gcccagaata ccctccttga cagtcttgac gtgcgcagct caggggcatg atgtgactgt
     2701 cgcccgtaca tttagcccat acatccccat gtataatcat ttgcatccat acattttgat
     2761 ggccgcacgg cgcgaagcaa aaattacggc tcctcgctgc agacctgcga gcagggaaac
     2821 gctcccctca cagacgcgtt gaattgtccc cacgccgcgc ccctgtagag aaatataaaa
     2881 ggttaggatt tgccactgag gttcttcttt catatacttc cttttaaaat cttgctagga
     2941 tacagttctc acatcacatc cgaacataaa caaccatggg taaggaaaag actcacgttt
     3001 cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg
     3061 ataatgtcgg gcaatcaggt gcgacaatct atcgattgta tgggaagccc gatgcgccag
     3121 agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca
     3181 gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc
     3241 ctgatgatgc atggttactc accactgcga tccccggcaa aacagcattc caggtattag
     3301 aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt
     3361 tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc
     3421 aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta
     3481 atggctggcc tgttgaacaa gtctggaaag aaatgcataa gcttttgcca ttctcaccgg
     3541 attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat
     3601 taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca
     3661 tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat
     3721 atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt
     3781 tctaatcagt actgacaata aaaagattct tgttttcaag aacttgtcat ttgtatagtt
     3841 tttttatatt gtagttgttc tattttaatc aaatgttagc gtgatttata ttttttttcg
     3901 cctcgacatc atctgcccag atgcgaagtt aagtgcgcag aaagtaatat catgcgtcaa
     3961 tcgtatgtga atgctggtcg ctatactgct gtcgattcga tactaacgcc gccatccagt
     4021 gtcgaaaacg agctcgaatt aattcctggg ggaacaactt cacagaatgt tttgtcatat
     4081 tgtcgaagtg gtcacaaaac aagagaagtt ccgccaatta taaaaaggga acccgtatat
     4141 ttcagcttca cggatgattt ccagggtgag agtactgtat atgggcttac gatagaaggc
     4201 cataaaaatt tcttgcttgg caacaaaata gaagtgaaat catgtcgagg ctgctgtgtg
     4261 ggagaacagc ataaaatatc acaaaaaaag aatctaaaac actgtgttgc ttgtcccaga
     4321 aagggaatca agtattttta taaagattgg agtggtaaaa atcgagtatg tgctagatgc
     4381 tatggaagat acaaattcag cggtcatcac tgtataaatt gcaagtatgt accagaagca
     4441 cgtgaagtga aaaaggcaaa agacaaaggc gaaaaattgg gcattacgcc cgaaggtttg
     4501 ccagttaaag gaccagagtg tataaaatgt ggcggaatct tacagtggcc tatgcggccg
     4561 ctctagctag aactagtgga tcgatcccca attcgcccta tagtgagtcg tattacaatt
     4621 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     4681 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     4741 gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc
     4801 ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg
     4861 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     4921 cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     4981 ctttccccgt caagctctaa atcggggtgg gccatcgccc tgatagacgg tttttcgccc
     5041 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     5101 caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg
     5161 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     5221 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     5281 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     5341 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     5401 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     5461 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     5521 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     5581 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     5641 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     5701 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     5761 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     5821 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     5881 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     5941 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     6001 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     6061 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     6121 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     6181 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     6241 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     6301 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     6361 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     6421 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     6481 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     6541 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     6601 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     6661 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     6721 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     6781 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     6841 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     6901 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcac agcggtcggg
     6961 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     7021 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     7081 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     7141 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     7201 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     7261 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     7321 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     7381 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     7441 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     7501 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     7561 acactttatg cttccgcggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     7621 acaggaaaca gctatgacca tgattacgcc actagtccga ggcctcgaga tccgatatcg
     7681 ccgtggcggc cgccagctga agcttaatta tcctgggcac gagtgaaaca aagctaaaac
     7741 ctttatttag catggccatt gaatgtaaca attatatata tcgcaagcac aaaaaatcaa
     7801 ggagagagaa ctaccacttt gttcatgtgt acaatgttca ttatctccat aagcaaaaaa
     7861 aaaaaaatag aaaacatatg ctataaggtt gatattctca cgagtaagcg gcacttgcta
     7921 cttattgaca ttgcagattt ttggctacag aaatagtata ttagagatta taattgctaa
     7981 tcaaatcaaa atataaaatt agtaaaccaa accatttata cccttcctta gtagttatgg
     8041 attgtttttt aatgatattt ctgcaaacca aagaaagatt gttatccaga tagaatttag
     8101 ttttgatatt catttttttg ttgaagattg aacgccatat ctgggcctca taattcaaaa
     8161 gacggtgcca ttatcggtag cgtttcgcat tgtactggat ttcagaaatt tcacagttga
     8221 tgaatcgaaa agaatggtct cattgcaaca cgtaaggtta agatgtccct ttttaccatt
     8281 ataggcaata aatgaatcat aaaacgaccg tatactggtg aaatagtagg gagaacgagt
     8341 acctgtagta aaaagtataa atcatagtta atcgggcaat gtccctcgat caaggagtat
     8401 tgtgtcatgt tcgagacaaa cgccaacatt tttgtttctt ttggacaaat gttgtttgca
     8461 tttatgatcc gttatatttt gatctaatgt agagttgcac gtagttctta ctggcaaaga
     8521 aatcgatgca taccaaaaaa gaataaaggt gatatttgat ctttaccgtt tagttccaac
     8581 gtaaaattgt gcctttggac ttaaaatggc ggggtgtaca atatggactt cctcttttct
     8641 ggcaaccaaa cccatacatc gggattccta taataccttc gttggtctcc ctaacatgta
     8701 ggtggcggag gggagatata caatagaaca gataccagac aagacataat gggctaaaca
     8761 agactacacc aattacactg cctcattgat ggtggtacat aacgaactaa tactgtagcc
     8821 ctagacttga tagccatcat catatcgaag tttcactacc ctttttccat ttgccatcta
     8881 ttgaagtaat aataggcgca tg
//
