LOCUS       sequence-27487-e         722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of pH-sensitive monomeric mNectarine red 
            fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Johnson DE, Ai HW, Wong P, Young JD, Campbell RE, Casey J
  TITLE     Red fluorescent protein pH biosensor to detect concentrative 
            nucleoside transport.
  JOURNAL   J Biol Chem. 2009 Jul 31;284(31):20499-511. doi: 
            10.1074/jbc.M109.019042.
  PUBMED    19494110
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1074/jbc.M109"; 
            journalName: "J Biol Chem"; date: "2009-07-31- 31"; volume: "284"; 
            issue: "31"; pages: "20499-511"
COMMENT     Sequence Label: pBAD-mNectarine
FEATURES             Location/Qualifiers
     source          1..722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             85..102
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             106..138
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(142..150,151..165)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(326..344)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 cctgacgctt tttatcgcaa ctctctactg tttctccata cccgtttttt gggctaacag
       61 gaggaattaa ccatgggggg ttctcatcat catcatcatc atggtatggc tagcatgact
      121 ggtggacagc aaatgggtcg ggatctgtac gacgatgacg ataaggatcc gagctcgagc
      181 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
      241 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggtga gggcgagggc
      301 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
      361 ttcgcctggg acatcctgtc ccctcagttc tgctacggct ccaaggccta tgtgaagcac
      421 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcctcaa ttgggagcgc
      481 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
      541 ggcgagttca tctacaaggt gaagctgcgc ggcactaact tcccctccga cggccccgta
      601 atgcagtgtc gtaccgtggg ctgggaggcc tccactgagc ggatgcaccc cgaggacggc
      661 gccctgaagg gcgagatcat gcagaggctg aagctgaagg gacggcggcc actacgacgc
      721 tg
//
