LOCUS       sequence-27489-e         624 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Strack RL, Bhattacharyya D, Glick BS, Keenan RJ
  TITLE     Noncytotoxic orange and red/green derivatives of DsRed-Express2 for 
            whole-cell labeling.
  JOURNAL   BMC Biotechnol. 2009 Apr 3;9:32. doi: 10.1186/1472-6750-9-32.
  PUBMED    19344508
REFERENCE   2  (bases 1 to 624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2009-04-3"; volume: "9:32. doi"; pages: " 
            10.1186/1472-6750-9-32"
COMMENT     Sequence Label: pE2-Orange-N1
FEATURES             Location/Qualifiers
     source          1..624
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    28..108
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      110..119
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     514..533
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gagctggttt agtgnaccgt cagatccgct agcgctaccg gactcagatc tcgagctcaa
       61 gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat ccactagtcg ccaccatgga
      121 tagcactgag aacgtcatca agcccttcat gcgcttcaag gtgcacatgg agggctccgt
      181 gaacggccac gagttcgaga tcgagggcga gggcgagggc aagccctacg agggcaccca
      241 gaccgccaag ctgcaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc
      301 cccccagttc acctacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta
      361 caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
      421 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcaccttca tctaccacgt
      481 gaagttcatc ggcgtgaact tcccctccga cggccccgta atgcagaaga agactctggg
      541 ctgggagccc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca
      601 caaggcgctg aagctgaagg gcgg
//
