LOCUS       sequence-27490-e         670 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Strack RL, Bhattacharyya D, Glick BS, Keenan RJ
  TITLE     Noncytotoxic orange and red/green derivatives of DsRed-Express2 for 
            whole-cell labeling.
  JOURNAL   BMC Biotechnol. 2009 Apr 3;9:32. doi: 10.1186/1472-6750-9-32.
  PUBMED    19344508
REFERENCE   2  (bases 1 to 670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2009-04-3"; volume: "9:32. doi"; pages: " 
            10.1186/1472-6750-9-32"
COMMENT     Sequence Label: pE2-Red/Green-N1
FEATURES             Location/Qualifiers
     source          1..670
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    25..105
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      107..116
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(293..313)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     511..530
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ctggttnagt ganccgtcag atccgctagc gctaccggnc tcagatctcg agctcaagct
       61 tcgaattctg cagtcgacgg taccgcgggc ccgggatcca ctagtcgcca ccatggatag
      121 cactgagaac gtcatcaagc ccttcatgcg cttcaaggtg cacatggagg gctccgtgaa
      181 cggccacgag ttcgagatcg agggcgaggg cgagggcaag ccctacgagg gcacccacac
      241 cgtcaagctg caggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc
      301 ccagttccag tacggctcca aggtgtacgt gaagcacccc gccgacatcc ccgactacaa
      361 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
      421 cgtggtgacc gtgacccagg actcctccct gcaggacggc accttcatct accacgtgaa
      481 gttcatcggc gtgaacttcc cctccgacgg ccccgtaatg cagaagaaga ctctgggctg
      541 ggagccctcc accgagcgcc tgtacccccg cgacggcgtg ctgaanggcg agatccacaa
      601 ggcgctgaag ctgaagggcg gcggccacta cctggtggag ttcaagtcaa tctacatggc
      661 caagaagccc
//
