LOCUS       sequence-274921-        3780 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Anderson promoter variant using mRFP as a reporter gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Teh MY, Ooi KH, Danny Teo SX, Bin Mansoor ME, Shaun Lim WZ, Tan MH
  TITLE     An Expanded Synthetic Biology Toolkit for Gene Expression Control in
            Acetobacteraceae.
  JOURNAL   ACS Synth Biol. 2019 Apr 19;8(4):708-723. doi: 
            10.1021/acssynbio.8b00168. Epub 2019 Mar 22.
  PUBMED    30865830
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.8b00168"; journalName: "ACS Synth Biol"; date: 
            "2019-04-19- 19"; volume: "8"; issue: "4"; pages: "708-723"
COMMENT     Sequence Label: MYMH 395
FEATURES             Location/Qualifiers
     source          1..3780
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        11..45
                     /label=BBa_J23109
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.04"
     RBS             53..64
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             71..748
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      793..864
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      880..907
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      939..1033
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        1081..1183
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1184..1843
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1250..1269)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     oriT            1992..2100
                     /note="incP origin of transfer"
     CDS             complement(2111..2773)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      2774..3545
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     terminator      complement(3660..3746)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     join(3761..3780,1..3)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 aggagaattc tttacagcta gctcagtcct agggactgtg ctagcactag tgaaagagga
       61 gaaatactag atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg
      121 tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc
      181 gtacgaaggt acccagaccg ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc
      241 ttgggacatc ctgtccccgc agttccagta cggttccaaa gcttacgtta aacacccggc
      301 tgacatcccg gactacctga aactgtcctt cccggaaggt ttcaaatggg aacgtgttat
      361 gaacttcgaa gacggtggtg ttgttaccgt tacccaggac tcctccctgc aagacggtga
      421 gttcatctac aaagttaaac tgcgtggtac caacttcccg tccgacggtc cggttatgca
      481 gaaaaaaacc atgggttggg aagcttccac cgaacgtatg tacccggaag acggtgctct
      541 gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt ggtcactacg acgctgaagt
      601 taaaaccacc tacatggcta aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat
      661 caaactggac atcacctccc acaacgaaga ctacaccatc gttgaacagt acgaacgtgc
      721 tgaaggtcgt cactccaccg gtgcttaata acgctgatag tgctagtgta gatcgctact
      781 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
      841 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
      901 ttctgcgttt atatactaga agcggccgct gcagcttgga ctcctgttga tagatccagt
      961 aatgacctca gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt
     1021 tattggtgag aatccagggg tccccaataa ttacgattta aattggcgaa aatgagacgt
     1081 tgatcggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt
     1141 attttttgag ttatcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     1201 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     1261 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     1321 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     1381 gatgaatgct catccggaat ttcgtatggc aatgaaagac ggtgagctgg tgatatggga
     1441 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     1501 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     1561 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     1621 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     1681 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     1741 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     1801 tgaattacaa cagtactgcg atgagtggca gggcggggcg taatttgact tttgtccttt
     1861 tccgctgcat aaccctgctt cggggtcatt atagcgattt tttcggtata tccatccttt
     1921 ttcgcacgat atacaggatt ttgccaaagg gttcgtgtag actttccttg gtgtatccaa
     1981 cggcgtcagc cgggcaggat aggtgaagta ggcccacccg cgagcgggtg ttccttcttc
     2041 actgtccctt attcgcacct ggcggtgctc aacgggaatc ctgctctgcg aggctggccg
     2101 taggccggcc ctaccggcgc ggcagcgtta cccgtgtcgg cggctccaac ggctcgccat
     2161 cgtccagaaa acacggctca tcgggcatcg gcaggcgctg ctgcccgcgc cgttcccatt
     2221 cctccgtttc ggtcaaggct ggcaggtctg gttccatgcc cggaatgccg ggctggctgg
     2281 gcggctcctc gccggggccg gtcggtagtt gctgctcgcc cggatacagg gtcgggatgc
     2341 ggcgcaggtc gccatgcccc aacagcgatt cgtcctggtc gtcgtgatca accaccacgg
     2401 cggcactgaa caccgacagg cgcaactggt cgcggggctg gccccacgcc acgcggtcat
     2461 tgaccacgta ggccgacacg gtgccggggc cgttgagctt cacgacggag atccagcgct
     2521 cggccaccaa gtccttgact gcgtattgga ccgtccgcaa agaacgtccg atgagcttgg
     2581 aaagtgtctt ctggctgacc accacggcgt tctggtggcc catctgcgcc acgaggtgat
     2641 gcagcagcat tgccgccgtg ggtttcctcg caataagccc ggcccacgcc tcatgcgctt
     2701 tgcgttccgt ttgcacccag tgaccgggct tgttcttggc ttgaatgccg atttctctgg
     2761 actgcgtggc catgcttatc tccatgcggt aggggtgccg cacggttgcg gcaccatgcg
     2821 caatcagctg caacttttcg gcagcgcgac aacaattatg cgttgcgtaa aagtggcagt
     2881 caattacaga ttttctttaa cctacgcaat gagctattgc ggggggtgcc gcaatgagct
     2941 gttgcgtacc cccctttttt aagttgttga tttttaagtc tttcgcattt cgccctatat
     3001 ctagttcttt ggtgcccaaa gaagggcacc cctgcggggt tcccccacgc cttcggcgcg
     3061 gctccccctc cggcaaaaag tggcccctcc ggggcttgtt gatcgactgc gcggccttcg
     3121 gccttgccca aggtggcgct gcccccttgg aacccccgca ctcgccgccg tgaggctcgg
     3181 ggggcaggcg ggcgggcttc gcccttcgac tgcccccact cgcataggct tgggtcgttc
     3241 caggcgcgtc aaggccaagc cgctgcgcgg tcgctgcgcg agccttgacc cgccttccac
     3301 ttggtgtcca accggcaagc gaagcgcgca ggccgcaggc cggaggcttt tccccagaga
     3361 aaattaaaaa aattgatggg gcaaggccgc aggccgcgca gttggagccg gtgggtatgt
     3421 ggtcgaaggc tgggtagccg gtgggcaatc cctgtggtca agctcgtggg caggcgcagc
     3481 ctgtccatca gcttgtccag cagggttgtc cacgggccga gcgaagcgag ccagccggtg
     3541 gccgctcgcg gccatcgtcc acatatccac gggctggcaa gggagcgcag cgaccgcgca
     3601 gggcgaagcc cggagagcaa gcccgtaggg ggggcgcgcc cagctgtcta gggcggcgga
     3661 tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct
     3721 ttcgactgag cctttcgttt tatttgatgc ctttaattaa agcggataac aatttcacac
//
