LOCUS       sequence-274926-       12099 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcription factor tagged with Sir4 used for Calling Cards 
            experiments for binding measurement .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12099)
  TITLE     Mitra lab plasmids
REFERENCE   2  (bases 1 to 12099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ADH1pro_GCR1_SIR4
FEATURES             Location/Qualifiers
     source          1..12099
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..705
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             4607..4636
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4643..4672
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4679..4708
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      5103..5290
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5158..5182)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     misc_feature    6120..6412
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     CDS             complement(7537..8631)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(8632..9039)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(9301..9319)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9387..9491
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9492..9560,9561..10352)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9710..9729)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10523..11111
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11012..11031
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11265..11282
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atccttttgt tgtttccggg tgtacaatat ggacttcctc ttttctggca accaaaccca
       61 tacatcggga ttcctataat accttcgttg gtctccctaa catgtaggtg gcggagggga
      121 gatatacaat agaacagata ccagacaaga cataatgggc taaacaagac tacaccaatt
      181 acactgcctc attgatggtg gtacataacg aactaatact gtagccctag acttgatagc
      241 catcatcata tcgaagtttc actacccttt ttccatttgc catctattga agtaataata
      301 ggcgcatgca acttcttttc tttttttttc ttttctctct cccccgttgt tgtctcacca
      361 tatccgcaat gacaaaaaaa tgatggaaga cactaaagga aaaaattaac gacaaagaca
      421 gcaccaacag atgtcgttgt tccagagctg atgaggggta tctcgaagca cacgaaactt
      481 tttccttcct tcattcacgc acactactct ctaatgagca acggtatacg gccttccttc
      541 cagttacttg aatttgaaat aaaaaaaagt ttgctgtctt gctatcaagt ataaatagac
      601 ctgcaattat taatcttttg tttcctcgtc attgttctcg ttccctttct tccttgtttc
      661 tttttctgca caatacttca agctatacca agcatacaat caactccaag cttgaagcaa
      721 gcctcctgaa agatggtatg gtatgatcat aacacgcatt ctgaaaatgt tatctgggag
      781 gttttcgatg ggtatggagt tttccttgat tctcacttat tatcccttgt attgtaattg
      841 atccttcagt aatatttgca gcctttcaca actatccttt ttttcattgc ttattactat
      901 tgaacctttt ttaggagttg cctgcttatg caatataatt tgctgacaag tagtaaatta
      961 ccagcacaat attaagatta aaaaagaaat tagccaagag cttgatatat tatcttatac
     1021 acaaaccttt ccgacctact tgataaagcc acatacctct acctcttcta ttagaaatag
     1081 aaaagtacaa aaatagcaaa aggaaataat ttctttaaaa taacattgtg tgaggttcca
     1141 actatggatt attaatagag taacgcaaac ttaaggaaag gaagtgcttt acaattaagt
     1201 atttataaga acgaatttat cccccaaaaa aaagcaccta tacttaataa aaggagggga
     1261 atagctatca attgagtgtt gtctgcgtct gtctgcgtac aagaggatga attttctgac
     1321 tcaggctatg tcagaaactt ttcaagggac aaataacagg ataaaacgta atgtcaggac
     1381 acaaagtgtg ccatcaactt cctataataa tggcaaagaa tcatatggac caaatactaa
     1441 ccaattaaat gccctacttt ctcaattgga acagcaaaca agtgttgata gtaccagcac
     1501 gagctcaaac ttttattcca ttgcacaata tattttacaa tcatacttca aggtcaatgt
     1561 agattctcta aactctctga aattggtgga tttgatagtg gaccaaactt accctgattc
     1621 tttgacgctg cgaaagctga atgaaggagc aacgggacaa ccatacgatt atttcaatac
     1681 agtttctcgt gatgctgata tctccaagtg tccaattttt gcgttgacca tattttttgt
     1741 tatacgatgg agccacccaa accctccaat ttcaattgag aattttacta cagtaccgtt
     1801 gctagattca aactttattt ctctaaattc caatccttta ctatatattc aaaatcaaaa
     1861 cccaaacagc aattcaagtg ttaaagtttc aaggtcacaa acgtttgaac cttctaaaga
     1921 gttgatcgat ttggtatttc catggctgtc ttatttgaag caggatatgc ttcttattga
     1981 taggacgaat tacaagcttt attctctctg tgaactattt gaatttatgg gcagggttgc
     2041 cattcaggat ctccgatatc tgagtcaaca tcccttatta ctacccaata tcgtaacatt
     2101 catttcaaaa tttattcctg agttattcca aaacgaagag tttaaaggaa tcggttcaat
     2161 taaaaattca aacaataatg ccctgaacaa tgttacagga atagaaaccc aatttttaaa
     2221 tccatctacc gaggaagtga gtcaaaaagt tgattcttac tttatggaat tatcaaaaaa
     2281 attaactaca gaaaatatca ggttaagtca agaaataaca caactaaaag cagatatgaa
     2341 ctccgtaggc aatgtttgta accaaatttt gctgttgcag agacaattgc tttcaggaaa
     2401 tcaggcgatc ggatcaaagt ccgaaaatat tgtgtcttcc acaggtgggg ggatattaat
     2461 actagataaa aatagcatca attcgaacgt actgagtaat ttggttcagt cgatagatcc
     2521 taatcactcc aagcccaacg gacaagccca aacacatcaa aggggtccga aaggacaatc
     2581 acatgcacag gttcaaagta ctaatagccc tgcgctagcg ccaattaaca tgttcccgag
     2641 cttaagtaat tctatacagc cgatgcttgg caccttggct ccgcaaccgc aagatatagt
     2701 acagaagagg aagctaccgt taccaggttc aatagcctct gcagcaacag gcagtccttt
     2761 ttctccatca cccgttggtg agtctcccta tagcaaacgc tttaaactag acgataaacc
     2821 aactccgtct cagacggctc ttgattcctt acttacaaaa tccatttcaa gccctagatt
     2881 acccctttcg acgttggcta acacagctgt cacggaatct tttcgctcac ctcagcagtt
     2941 tcagcattct ccagattttg tagttggtgg tagctcaagt tcaacaacgg aaaataactc
     3001 taagaaggta aatgaagatt ctccatcatc ttcttcaaaa ctagctgaac gacctcgtct
     3061 tccaaacaac gactccacta ctagcatgcc tgaaagtccc accgaggtag ctggtgatga
     3121 tgttgatagg gagaaaccgc cagagtcaag taagtcggag cccaatgata acagcccaga
     3181 atcgaaagat cctgagaaaa atggtaaaaa cagtaatccg cttggtacgg atgctgacaa
     3241 accagtacca atttctaata ttcataattc tactgaggct gcaaattcaa gtggtacagt
     3301 gacaaagaca gctccatcat ttccgcagag ttcttctaag tttgaaatta taaataaaaa
     3361 ggatacgaag gcggggccaa acgaggcaat caaatacaag ctgtccagag aaaataaaac
     3421 aatatgggac ctatatgcgg agtggtatat tggtctgaac ggtaaatctt caataaaaaa
     3481 attgattgaa aattatggct ggcgaaggtg gaaggttagc gaagattcac atttttttcc
     3541 tactagaaga attattatgg attatattga aacggaatgt gatcgtggca taaaactcgg
     3601 caggtttact aatcctcaac aaccgaggga ggatatacgg aagattttag taggggacct
     3661 agaaaagttc aggataaata acggtctgac tctgaattct ctatcattgt actttagaaa
     3721 tttaacgaaa aataacaagg aaatttgtat ttttgaaaac tttaaaaatt ggaacgttag
     3781 atcaatgaca gaagaagaga aattaaagta ttgcaaaagg cgacataata caccatctag
     3841 agtgtcgcat agtgatacta attctagcaa acccaaaaac acgaaggaga acctttcaaa
     3901 aagctcttgg aggcaagaat ggcttgcaaa tttgaaactt atttccgttt cgttggttga
     3961 tgagttccct tcggagcttt ccgacagtga tagacaaata attaacgaaa aaatgcagtt
     4021 acttaaagat atatttgcta acaaccttaa atcagcaatt tccaataatt ttagagagag
     4081 tgacatcatt atactgaaag gtgaaataga agattaccca atgagttctg aaattaagat
     4141 ttactacaac gaattacaga acaagcctga tgcaaaaaaa gccagatttt ggtcatttat
     4201 gaagactcag agatttgttt ccaacatggg atttgatatt cagaagtcct gtgaacctgt
     4261 ttctatatcc acgtcggtca aaccacatgt ggtcgagccc gagcatatgg cagatgccaa
     4321 aattatgcct aaggatatac tgcaaattac aaaaaaaccg ttaatggtta aaaatgtgaa
     4381 gccttcttct ccgccagatg tgaagtcttt ggtgcagctg agcacaatgg aaacgaaaac
     4441 cctaccagaa aagaagcaat tcgacagtat ttttaattct aacaaagcaa aaataatccc
     4501 tggaaatggg aagcacgcat cagaaaacat ctcactctct ttctcaagac ctgcgtccta
     4561 cggctatttt tctgttggaa aaagggttcg gggatcctct agaggtgaac aaaagttgat
     4621 ttctgaagaa gatttgaacg gtgaacaaaa gctaatctcc gaggaagact tgaacggtga
     4681 acaaaaatta atctcagaag aagacttgaa cggatcctct agagtcgact gaatcgtaga
     4741 tactgaaaaa ccccgcaagt tcacttcaac tgtgcatcgt gcaccatctc aatttctttc
     4801 atttatacat cgttttgcct tcttttatgt aactatactc ctctaagttt caatcttggc
     4861 catgtaacct ctgatctata gaatttttta aatgactaga attaatgccc atcttttttt
     4921 tggacctaaa ttcttcatga aaatatatta cgagggctta ttcagaagct ttggacttct
     4981 tcgccagagg tttggtcaag tctccaatca aggttgtcgg cttgtctacc ttgccagaaa
     5041 tttacgaaaa gatggaaaag ggtcaaatcg ttggtagata cgttgttgac acttctaaat
     5101 aagcgaattt cttatgattt atgattttta ttattaaata agttataaaa aaaataagtg
     5161 tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa attcttattc ttgagtaact
     5221 ctttcctgta ggtcaggttg ctttctcagg tatagcatga ggtcgctctt attgaccaca
     5281 cctctaccgg catgccgagc aaatgcctgc aaatcgctcc ccatttcacc caattgtaga
     5341 tatgctaact ccagcaatga gttgatgaat ctcggtgtgt attttatgtc ctcagaggac
     5401 aacacctgtt gtaatcgttc ttccacacgg atcctggcgt aatagcgaag aggcccgcac
     5461 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt
     5521 tctccttacg catctgtgcg gtatttcaca ccgcataatc gctgggccat tctcatgaag
     5581 aatatcttga atttattgtc atattactag ttggtgtgga agtcctaata tcggtgatca
     5641 atatagtggt tgacatgctg gctagtcaac attgagcctt ttgatcatgc aaatatatta
     5701 cggtatttta caatcaaata tcaaacttaa ctattgactt tataacttat ttaggtggta
     5761 acattcttat aaaaaagaaa aaaattactg caaaacagta ctagctttta acttgtatcc
     5821 taggttatct atgctgtctc accatagaga atattaccta tttcagaatg tatgtccatg
     5881 attcgccggg taaatacata taatacacaa atctggctta ataaagtcta taatatatct
     5941 cataaagaag tgctaaattg gctagtgcta tatattttta agaaaatttc ttttgactaa
     6001 gtccatatcg actttgtaaa agttcacatt agcatacata tattacacga gccagaaata
     6061 gtaacttttg cctaaatcac aaattgcaaa atttaattgc ttgcaaaagg tcacatgctt
     6121 ataatcaact tttttaaaaa tttaaaatac ttttttattt tttattttta aacataaatg
     6181 aaataattta tttattgttt atgattaccg aaacataaaa cctgctcaag aaaaagaaac
     6241 tgttttgtcc ttggaaaaaa agcactacct aggagcggcc aaaatgccga ggctttcata
     6301 gcttaaactc tttacagaaa ataggcatta tagatcagtt cgagttttct tattcttcct
     6361 tccggtttta tcgtcacagt tttacagtaa ataagtatca cctcttagag ttcgatgata
     6421 agctgtcaaa catgagaatt aattccacat gttaaaatag tgaaggagca tgttcggcac
     6481 acagtggacc gaacgtgggg taagtgcact agggtccggt taaacggatc tcgcattgat
     6541 gaggcaacgc taattatcaa catatagatt gttatctatc tgcatgaaca cgaaatcttt
     6601 acttgacgac ttgaggctga tggtgtttat gcaaagaaac cactgtgttt aatatgtgtc
     6661 actgtttgat attactgtca gcgtagaaga taatagtaaa agcggttaat aagtgtattt
     6721 gagataagtg tgataaagtt tttacagcga aaagacgata aatacaagaa aatgattacg
     6781 aggatacgga gagaggtatg tacatgtgta tttatatact aagctgccgg cggttgtttg
     6841 caagaccgag aaaaggctag caagaatcgg gtcattgtag cgtatgcgcc tgtgaacatt
     6901 ctcttcaaca agtttgattc cattgcggtg aaatggtaaa agtcaacccc ctgcgatgta
     6961 tattttcctg tacaatcaat caaaaagcca aatgatttag cattatcttt acatcttgtt
     7021 attttacaga ttttatgttt agatctttta tgcttgcttt tcaaaaggct tgcaaggccg
     7081 tttctgacag agtaaaattc ttgagggaac tttcaccatt atgggaaatg gttcaagaag
     7141 gtattgactt aaactccatc aaatggtcag gtcattgagt gttttttatt tgttgtattt
     7201 tttttttttt agagaaaatc ctccaatata taaattagga atcatagttt catgattttc
     7261 tgttacacct aactttttgt gtggtgccct cctccttgtc aatattaatg ttaaagtgca
     7321 attctttttc cttatcacgt tgagccatta gtatcaattt gcttacctgt attcctttac
     7381 atcctccttt ttctccttct tgataaatgt atgtagattg cgtatatagt ttcgtctacc
     7441 ctatgaacat attccatttt gtaatttcgt gtcgtttcta ttatgaattt catttataaa
     7501 gtttatgtac aaatatcata aaaaaagaga atctttttaa gcaaggattt tcttaacttc
     7561 ttcggcgaca gcatcaccga cttcggtggt actgttggaa ccacctaaat caccagttct
     7621 gatacctgca tccaaaacct ttttaactgc atcttcaatg gccttacctt cttcaggcaa
     7681 gttcaatgac aatttcaaca tcattgcagc agacaagata gtggcgatag ggttgacctt
     7741 attctttggc aaatctggag cagaaccgtg gcatggttcg tacaaaccaa atgcggtgtt
     7801 cttgtctggc aaagaggcca aggacgcaga tggcaacaaa cccaaggaac ctgggataac
     7861 ggaggcttca tcggagatga tatcaccaaa catgttgctg gtgattataa taccatttag
     7921 gtgggttggg ttcttaacta ggatcatggc ggcagaatca atcaattgat gttgaacctt
     7981 caatgtaggg aattcgttct tgatggtttc ctccacagtt tttctccata atcttgaaga
     8041 ggccaaaaca ttagctttat ccaaggacca aataggcaat ggtggctcat gttgtagggc
     8101 catgaaagcg gccattcttg tgattctttg cacttctgga acggtgtatt gttcactatc
     8161 ccaagcgaca ccatcaccat cgtcttcctt tctcttacca aagtaaatac ctcccactaa
     8221 ttctctgaca acaacgaagt cagtaccttt agcaaattgt ggcttgattg gagataagtc
     8281 taaaagagag tcggatgcaa agttacatgg tcttaagttg gcgtacaatt gaagttcttt
     8341 acggattttt agtaaacctt gttcaggtct aacactaccg gtaccccatt taggaccacc
     8401 cacagcacct aacaaaacgg catcagcctt cttggaggct tccagcgcct catctggaag
     8461 tggaacacct gtagcatcga tagcagcacc accaattaaa tgattttcga aatcgaactt
     8521 gacattggaa cgaacatcag aaatagcttt aagaacctta atggcttcgg ctgtgatttc
     8581 ttgaccaacg tggtcacctg gcaaaacgac gatcttctta ggggcagaca ttagaatggt
     8641 atatccttga aatatatata tatatattgc tgaaatgtaa aaggtaagaa aagttagaaa
     8701 gtaagacgat tgctaaccac ctattggaaa aaacaatagg tccttaaata atattgtcaa
     8761 cttcaagtat tgtgatgcaa gcatttagtc atgaacgctt ctctattcta tatgaaaagc
     8821 cggttccggc gctctcacct ttcctttttc tcccaatttt tcagttgaaa aaggtatatg
     8881 cgtcaggcga cctctgaaat taacaaaaaa tttccagtca tcgaatttga ttctgtgcga
     8941 tagcgcccct gtgtgttctc gttatgttga ggaaaaaaat aatggttgct aagagattcg
     9001 aactcttgca tcttacgata cctgagtatt cccacagtta actgcggtca agatatttct
     9061 tgaatcaggc gccttagacc gctcggccaa acaaccaatt acttgttgag aaatagagta
     9121 taattatcct ataaatataa cgtttttgaa cacacatgaa caaggaagta caggacaatt
     9181 gattttgaag agaatgtgga ttttgatgta attgttggga ttccattttt aataaggcaa
     9241 taatattagg tatgtagata tactagaagt tctcctcgag gaattcttga agacgaaagg
     9301 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     9361 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     9421 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     9481 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     9541 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     9601 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     9661 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     9721 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     9781 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     9841 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     9901 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     9961 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
    10021 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
    10081 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
    10141 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
    10201 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
    10261 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
    10321 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
    10381 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
    10441 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
    10501 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
    10561 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
    10621 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
    10681 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
    10741 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
    10801 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    10861 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    10921 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    10981 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    11041 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    11101 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
    11161 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
    11221 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
    11281 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
    11341 aatgcaggat ccgggatcga agaaatgatg gtaaatgaaa taggaaatca aggagcatga
    11401 aggcaaaaga caaatataag ggtcgaacga aaaataaagt gaaaagtgtt gatatgatgt
    11461 atttggcttt gcggcgccga aaaaacgagt ttacgcaatt gcacaatcat gctgactctg
    11521 tggcggaccc gcgctcttgc cggcccggcg ataacgctgg gcgtgaggct gtgcccggcg
    11581 gagttttttg cgcctgcatt ttccaaggtt taccctgcgc taaggggcga gattggagaa
    11641 gcaataagaa tgccggttgg ggttgcgatg atgacgacca cgacaactgg tgtcattatt
    11701 taagttgccg aaagaacctg agtgcatttg caacatgagt atactagaag aatgagccaa
    11761 gacttgcgag acgcgagttt gccggtggtg cgaacaatag agcgaccatg accttgaagg
    11821 tgagacgcgc ataaccgcta gagtactttg aagaggaaac agcaataggg ttgctaccag
    11881 tataaataga caggtacata caacactgga aatggttgtc tgtttgagta cgctttcaat
    11941 tcatttgggt gtgcacttta ttatgttaca atatggaagg gaactttaca cttctcctat
    12001 gcacatatat taattaaagt ccaatgctag tagagaaggg gggtaacacc cctccgcgct
    12061 cttttccgat ttttttctaa accgtggaat atttcggat
//
