LOCUS       sequence-274969-        2241 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plux - STAR3 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Glasscock CJ, Biggs BW, Lazar JT, Arnold JH, Burdette LA, Valdes A, 
            Kang MK, Tullman-Ercek D, Tyo KEJ, Lucks JB
  TITLE     Dynamic Control of Gene Expression with Riboregulated Switchable 
            Feedback Promoters.
  JOURNAL   ACS Synth Biol. 2021 May 21;10(5):1199-1213. doi: 
            10.1021/acssynbio.1c00015. Epub 2021 Apr 9.
  PUBMED    33834762
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00015"; journalName: "ACS Synth Biol"; date: 
            "2021-05-21- 21"; volume: "10"; issue: "5"; pages: "1199-1213"
COMMENT     Sequence Label: pJBl6715
FEATURES             Location/Qualifiers
     source          1..2241
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(94..885,886..954))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIATLGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     717..736
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(955..1059)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1127..1145
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1149..1192)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(1405..1422)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1576..2164)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1656..1675)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
       61 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
      121 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
      181 aactacgata cgggagggct taccatctgg ccccagtgtt gcaatgatac cgcgagaccc
      241 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
      301 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
      361 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
      421 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
      481 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
      541 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
      601 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
      661 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
      721 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
      781 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
      841 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
      901 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
      961 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1021 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1081 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     1141 gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc
     1201 taaagatcta tatactagag acctgtagga tcgtacaggt ttacgcaaga aaatggtttg
     1261 ttatagtcga ataaatgaac tgtatacatt ccccgcaaag acacaggaca ggacaggcag
     1321 atagatagga tagaggggat ctcaaagccc gccgaaaggc gggctttttt ttggatcctt
     1381 actcgagtct agactgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     1441 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     1501 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     1561 cgcgttgctg gcgtttttcc acaggctccg cccccctgac gagcatcaca aaaatcgacg
     1621 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     1681 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     1741 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     1801 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     1861 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     1921 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     1981 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     2041 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     2101 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     2161 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     2221 ttaagggatt ttggtcatga g
//
