LOCUS       sequence-274989-        1154 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  empty GFP vector for cloning sgRNA or pgRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Esposito R, Polidori T, Meise DF, Pulido-Quetglas C, Chouvardas P, 
            Forster S, Schaerer P, Kobel A, Schlatter J, Kerkhof E, Roemmele M, 
            Rice ES, Zhu L, Lanzos A, Guillen-Ramirez HA, Basile G, Carrozzo I, 
            Vancura A, Ullrich S, Andrades A, Harvey D, Medina PP, Ma PC, 
            Haefliger S, Wang X, Martinez I, Ochsenbein AF, Riether C, Johnson R
  TITLE     Multi-hallmark long noncoding RNA maps reveal non-small cell lung 
            cancer vulnerabilities.
  JOURNAL   Cell Genom. 2022 Aug 22;2(9):100171. doi: 
            10.1016/j.xgen.2022.100171. eCollection 2022 Sep 14.
  PUBMED    36778670
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Genom."; date: "2022-08-22"; volume: "2(9):100171. doi"; pages: " 
            10.1016/j.xgen.2022.100171. eCollection 2022 Sep 14"
COMMENT     Sequence Label: pDECKO_GFP
FEATURES             Location/Qualifiers
     source          1..1154
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(17..38)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(join(173..685,686..688,689..691))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKLSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     366..385
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     625..646
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      688..697
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(753..772)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(754..772)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(817..1020)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(846..866)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tgancccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt
       61 ctttgctcag ggcggactgg gtgctcaggt agtggttgtc gggcagcagc acggggccgt
      121 cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgctgccg tcctcgatgt
      181 tgtggcggat cttgaagttc accttgatgc cgttcttctg cttgtcggcc atgatataga
      241 cgttgtggct gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga
      301 agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg
      361 cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt
      421 cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc
      481 actgcacgcc gtaggtcagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg
      541 tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg
      601 acacgctgag cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc
      661 cggtgaacag ctcctcgccc ttgctcacca tggtggcggc ggatccgagc tcggtaccaa
      721 gcttaagttt aaacgctagc cagcttgggt ctccctatag tgagtcgtat taatttcgat
      781 aagccagtaa gcagtgggtt ctctagttag ccagagagct ctgcttatat agacctccca
      841 ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac attttggaaa
      901 gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt ggagacttgg
      961 aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa accgcatcac
     1021 catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt
     1081 catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt
     1141 acttggcmta tgaw
//
