LOCUS       sequence-274990-         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  empty GFP vector for cloning sgRNA or pgRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Esposito R, Polidori T, Meise DF, Pulido-Quetglas C, Chouvardas P, 
            Forster S, Schaerer P, Kobel A, Schlatter J, Kerkhof E, Roemmele M, 
            Rice ES, Zhu L, Lanzos A, Guillen-Ramirez HA, Basile G, Carrozzo I, 
            Vancura A, Ullrich S, Andrades A, Harvey D, Medina PP, Ma PC, 
            Haefliger S, Wang X, Martinez I, Ochsenbein AF, Riether C, Johnson R
  TITLE     Multi-hallmark long noncoding RNA maps reveal non-small cell lung 
            cancer vulnerabilities.
  JOURNAL   Cell Genom. 2022 Aug 22;2(9):100171. doi: 
            10.1016/j.xgen.2022.100171. eCollection 2022 Sep 14.
  PUBMED    36778670
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Genom."; date: "2022-08-22"; volume: "2(9):100171. doi"; pages: " 
            10.1016/j.xgen.2022.100171. eCollection 2022 Sep 14"
COMMENT     Sequence Label: pDECKO_GFP
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(230..249)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     577..598
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             641..658
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(755..772)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    761..872
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 caagctcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
       61 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
      121 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
      181 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
      241 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
      301 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
      361 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
      421 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
      481 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
      541 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
      601 cgccgccggg atcactctcg gcatggacga gctgtacaag catcatcacc atcaccacta
      661 aggatccact agtccagtgt ggtggaattc tgcagatatc cagcacagtg gcggccgctc
      721 gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
      781 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
      841 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
      901 ctggggggtk ggggtggggc aggacagcaa ggggggagga ttgggaa
//
