LOCUS       sequence-274994-       10614 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SIX1 expression. Vector also expresses GFP under control of hUBC 
            promoter .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10614)
  TITLE     Kingsbury Lab Plasmids
REFERENCE   2  (bases 1 to 10614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pTJK299
FEATURES             Location/Qualifiers
     source          1..10614
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        255..634
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        635..838
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     789..809
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             853..1033
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1080..1205
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1698..1931
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2116..2160
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2453..2570
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2622..3800
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2853..3791
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3748..3768
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        4695..5906
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5743..5762
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(5913..5915,5916..5918,5919..6632)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5913..5915,5916..5918,5919..6629)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5958..5979)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6219..6238)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6566..6587
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6711..6731)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7129..7140)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7314..7547
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7619..7740
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7656..7675)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7710..7729
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7780..7915
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7935..7954)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7936..7954)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7964..7981)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7964..7980)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7973..7995)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8122..8577
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8209..8228)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8419..8440
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8603..8707
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8708..8776,8777..9568)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8926..8945)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9739..10327
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10228..10247
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10481..10498
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagcttg
      181 gccattgcat acgttgtatc catatcataa tatgtacatt tatattggct catgtccaac
      241 attaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
      301 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
      361 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
      421 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
      481 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
      541 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
      601 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
      661 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
      721 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
      781 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
      841 tttagtgaac cggggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
      901 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
      961 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     1021 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     1081 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     1141 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     1201 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     1261 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     1321 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     1381 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     1441 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     1501 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
     1561 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     1621 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     1681 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     1741 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     1801 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     1861 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     1921 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     1981 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     2041 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     2101 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     2161 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     2221 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     2281 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
     2341 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
     2401 aacggatctc gacggtatcg atctcgacac aaatggcagt attcatccac aattttaaaa
     2461 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     2521 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     2581 acagggacag cagagatcca gtttgggtcg aggattcgtg aggctccggt gcccgtcagt
     2641 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2701 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2761 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2821 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2881 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2941 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     3001 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     3061 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     3121 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     3181 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     3241 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     3301 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     3361 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3421 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3481 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3541 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3601 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3661 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3721 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3781 ttccatttca ggtgtcgtga gctagcccac catgtcgatg ctgccgtcgt ttggctttac
     3841 gcaggagcaa gtggcgtgcg tgtgcgaggt tctgcagcaa ggcggaaacc tggagcgcct
     3901 gggcaggttc ctgtggtcac tgcccgcctg cgaccacctg cacaagaacg agagcgtact
     3961 caaggccaag gcggtggtcg ccttccaccg cggcaacttc cgtgagctct acaagatcct
     4021 ggagagccac cagttctcgc ctcacaacca ccccaaactg cagcaactgt ggctgaaggc
     4081 gcattacgtg gaggccgaga agctgcgcgg ccgacccctg ggcgccgtgg gcaaatatcg
     4141 ggtgcgccga aaatttccac tgccgcgcac catctgggac ggcgaggaga ccagctactg
     4201 cttcaaggag aagtcgaggg gtgtcctgcg ggagtggtac gcgcacaatc cctacccatc
     4261 gccgcgtgag aagcgggagc tggccgaggc caccggcctc accaccaccc aggtcagcaa
     4321 ctggtttaag aaccggaggc aaagagaccg ggccgcggag gccaaggaaa gggagaacac
     4381 cgaaaacaat aactcctcct ccaacaagca gaaccaactc tctcctctgg aagggggcaa
     4441 gccgctcatg tccagctcag aagaggaatt ctcacctccc caaagtccag accagaactc
     4501 ggtccttctg ctgcagggca atatgggcca cgccaggagc tcaaactatt ctctcccggg
     4561 cttaacagcc tcgcagccca gtcacggcct gcagacccac cagcatcagc tccaagactc
     4621 tctgctcggc cccctcacct ccagtctggt ggacttgggg tcctaaccgg tgtcgacgga
     4681 tcccccgggc tgcaggaatt cgcgccgggt tttggcgcct cccgcgggcg cccccctcct
     4741 cacggcgagc gctgccacgt cagacgaagg gcgcagcgag cgtcctgatc cttccgcccg
     4801 gacgctcagg acagcggccc gctgctcata agactcggcc ttagaacccc agtatcagca
     4861 gaaggacatt ttaggacggg acttgggtga ctctagggca ctggttttct ttccagagag
     4921 cggaacaggc gaggaaaagt agtcccttct cggcgattct gcggagggat ctccgtgggg
     4981 cggtgaacgc cgatgattat ataaggacgc gccgggtgtg gcacagctag ttccgtcgca
     5041 gccgggattt gggtcgcggt tcttgtttgt ggatcgctgt gatcgtcact tggtgagtag
     5101 cgggctgctg ggctggccgg ggctttcgtg gccgccgggc cgctcggtgg gacggaagcg
     5161 tgtggagaga ccgccaaggg ctgtagtctg ggtccgcgag caaggttgcc ctgaactggg
     5221 ggttgggggg agcgcagcaa aatggcggct gttcccgagt cttgaatgga agacgcttgt
     5281 gaggcgggct gtgaggtcgt tgaaacaagg tggggggcat ggtgggcggc aagaacccaa
     5341 ggtcttgagg ccttcgctaa tgcgggaaag ctcttattcg ggtgagatgg gctggggcac
     5401 catctgggga ccctgacgtg aagtttgtca ctgactggag aactcggttt gtcgtctgtt
     5461 gcgggggcgg cagttatggc ggtgccgttg ggcagtgcac ccgtaccttt gggagcgcgc
     5521 gccctcgtcg tgtcgtgacg tcacccgttc tgttggctta taatgcaggg tggggccacc
     5581 tgccggtagg tgtgcggtag gcttttctcc gtcgcaggac gcagggttcg ggcctagggt
     5641 aggctctcct gaatcgacag gcgccggacc tctggtgagg ggagggataa gtgaggcgtc
     5701 agtttctttg gtcggtttta tgtacctatc ttcttaagta gctgaagctc cggttttgaa
     5761 ctatgcgctc ggggttggcg agtgtgtttt gtgaagtttt ttaggcacct tttgaaatgt
     5821 aatcatttgg gtcaatatgt aattttcagt gttagactag taaattgtcc gctaaattct
     5881 ggccgttttt ggcttttttg ttagacctcg agatggtgag caagggcgag gagctgttca
     5941 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     6001 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     6061 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     6121 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     6181 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     6241 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     6301 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     6361 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     6421 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     6481 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     6541 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     6601 tcactctcgg catggacgag ctgtacaagt aaggatatcg gtaccgcatg cgtttaaact
     6661 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     6721 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     6781 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     6841 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     6901 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     6961 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     7021 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     7081 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     7141 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     7201 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcactt taagaccaat
     7261 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     7321 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
     7381 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     7441 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     7501 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     7561 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     7621 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     7681 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     7741 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     7801 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     7861 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg
     7921 acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
     7981 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     8041 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     8101 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     8161 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     8221 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     8281 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     8341 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     8401 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     8461 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     8521 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
     8581 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     8641 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     8701 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     8761 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     8821 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     8881 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     8941 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     9001 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     9061 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     9121 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     9181 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     9241 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     9301 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     9361 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     9421 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     9481 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     9541 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     9601 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     9661 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     9721 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     9781 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     9841 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     9901 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     9961 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
    10021 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
    10081 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
    10141 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
    10201 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
    10261 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
    10321 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
    10381 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
    10441 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
    10501 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
    10561 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caat
//
