LOCUS       sequence-274995-       10991 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GATA1 expression. Vector also expresses GFP under control of hUBC 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10991)
  TITLE     Kingsbury Lab Plasmids
REFERENCE   2  (bases 1 to 10991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pTJK482
FEATURES             Location/Qualifiers
     source          1..10991
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        255..634
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        635..838
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     789..809
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             853..1033
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1080..1205
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1698..1931
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2116..2160
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2453..2570
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2622..3800
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2853..3791
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3748..3768
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        5072..6283
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     6120..6139
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(6290..6292,6293..6295,6296..7009)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6290..6292,6293..6295,6296..7006)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6335..6356)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6596..6615)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6943..6964
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(7088..7108)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7506..7517)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7691..7924
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7996..8117
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8033..8052)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8087..8106
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8157..8292
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8312..8331)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8313..8331)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8341..8358)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8341..8357)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8350..8372)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8499..8954
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8586..8605)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8796..8817
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8980..9084
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9085..9153,9154..9945)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9303..9322)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10116..10704
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10605..10624
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10858..10875
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagcttg
      181 gccattgcat acgttgtatc catatcataa tatgtacatt tatattggct catgtccaac
      241 attaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
      301 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
      361 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
      421 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
      481 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
      541 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
      601 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
      661 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
      721 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
      781 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
      841 tttagtgaac cggggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
      901 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
      961 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     1021 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     1081 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     1141 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     1201 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     1261 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     1321 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     1381 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     1441 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     1501 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
     1561 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     1621 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     1681 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     1741 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     1801 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     1861 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     1921 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     1981 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     2041 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     2101 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     2161 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     2221 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     2281 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
     2341 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
     2401 aacggatctc gacggtatcg atctcgacac aaatggcagt attcatccac aattttaaaa
     2461 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     2521 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     2581 acagggacag cagagatcca gtttgggtcg aggattcgtg aggctccggt gcccgtcagt
     2641 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2701 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2761 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2821 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2881 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2941 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     3001 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     3061 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     3121 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     3181 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     3241 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     3301 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     3361 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3421 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3481 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3541 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3601 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3661 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3721 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3781 ttccatttca ggtgtcgtga gctagcatgg agttccctgg cctggggtcc ctggggacct
     3841 cagagcccct cccccagttt gtggatcctg ctctggtgtc ctccacacca gaatcagggg
     3901 ttttcttccc ctctgggcct gagggcttgg atgcagcagc ttcctccact gccccgagca
     3961 cagccaccgc tgcagctgcg gcactggcct actacaggga cgctgaggcc tacagacact
     4021 ccccagtctt tcaggtgtac ccattgctca actgtatgga ggggatccca gggggctcac
     4081 catatgccgg ctgggcctac ggcaagacgg ggctctaccc tgcctcaact gtgtgtccca
     4141 cccgcgagga ctctcctccc caggccgtgg aagatctgga tggaaaaggc agcaccagct
     4201 tcctggagac tttgaagaca gagcggctga gcccagacct cctgaccctg ggacctgcac
     4261 tgccttcatc actccctgtc cccaatagtg cttatggggg ccctgacttt tccagtacct
     4321 tcttttctcc caccgggagc cccctcaatt cagcagccta ttcctctccc aagcttcgtg
     4381 gaactctccc cctgcctccc tgtgaggcca gggagtgtgt gaactgcgga gcaacagcca
     4441 ctccactgtg gcggagggac aggacaggcc actacctatg caacgcctgc ggcctctatc
     4501 acaagatgaa tgggcagaac aggcccctca tccggcccaa gaagcgcctg attgtcagta
     4561 aacgggcagg tactcagtgc accaactgcc agacgaccac cacgacactg tggcggagaa
     4621 atgccagtgg ggatcccgtg tgcaatgcct gcggcctcta ctacaagcta caccaggtga
     4681 accggccact gaccatgcgg aaggatggta ttcagactcg aaaccgcaag gcatctggaa
     4741 aagggaaaaa gaaacggggc tccagtctgg gaggcacagg agcagccgaa ggaccagctg
     4801 gtggctttat ggtggtggct gggggcagcg gtagcgggaa ttgtggggag gtggcttcag
     4861 gcctgacact gggcccccca ggtactgccc atctctacca aggcctgggc cctgtggtgc
     4921 tgtcagggcc tgttagccac ctcatgcctt tccctggacc cctactgggc tcacccacgg
     4981 gctccttccc cacaggcccc atgcccccca ccaccagcac tactgtggtg gctccgctca
     5041 gctcatgagt cgacggatcc cccgggctgc aggaattcgc gccgggtttt ggcgcctccc
     5101 gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt
     5161 cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga ctcggcctta
     5221 gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc tagggcactg
     5281 gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg cgattctgcg
     5341 gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc gggtgtggca
     5401 cagctagttc cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga tcgctgtgat
     5461 cgtcacttgg tgagtagcgg gctgctgggc tggccggggc tttcgtggcc gccgggccgc
     5521 tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa
     5581 ggttgccctg aactgggggt tggggggagc gcagcaaaat ggcggctgtt cccgagtctt
     5641 gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt
     5701 gggcggcaag aacccaaggt cttgaggcct tcgctaatgc gggaaagctc ttattcgggt
     5761 gagatgggct ggggcaccat ctggggaccc tgacgtgaag tttgtcactg actggagaac
     5821 tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt gccgttgggc agtgcacccg
     5881 tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa
     5941 tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct tttctccgtc gcaggacgca
     6001 gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg ccggacctct ggtgagggga
     6061 gggataagtg aggcgtcagt ttctttggtc ggttttatgt acctatcttc ttaagtagct
     6121 gaagctccgg ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg aagtttttta
     6181 ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt agactagtaa
     6241 attgtccgct aaattctggc cgtttttggc ttttttgtta gacctcgaga tggtgagcaa
     6301 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     6361 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     6421 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     6481 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     6541 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     6601 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     6661 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     6721 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     6781 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     6841 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     6901 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     6961 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaag gatatcggta
     7021 ccgcatgcgt ttaaactcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     7081 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     7141 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     7201 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     7261 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     7321 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     7381 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     7441 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     7501 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     7561 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     7621 cgcactttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     7681 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     7741 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     7801 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     7861 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     7921 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     7981 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     8041 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     8101 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc
     8161 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     8221 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     8281 tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc
     8341 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     8401 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     8461 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt
     8521 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     8581 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     8641 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     8701 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     8761 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     8821 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     8881 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     8941 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     9001 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     9061 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     9121 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     9181 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     9241 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     9301 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     9361 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     9421 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     9481 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     9541 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     9601 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     9661 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     9721 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     9781 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     9841 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     9901 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     9961 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
    10021 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
    10081 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
    10141 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
    10201 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
    10261 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
    10321 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
    10381 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
    10441 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
    10501 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
    10561 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
    10621 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
    10681 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
    10741 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
    10801 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
    10861 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
    10921 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
    10981 cgcaacgcaa t
//
