LOCUS       sequence-274997-       11528 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EYA1 expression. Vector also expresses GFP under control of hUBC 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11528)
  TITLE     Kingsbury Lab Plasmids
REFERENCE   2  (bases 1 to 11528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pTJK460
FEATURES             Location/Qualifiers
     source          1..11528
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        255..634
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        635..838
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     789..809
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             853..1033
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1080..1205
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1698..1931
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2116..2160
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2453..2570
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2622..3800
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2853..3791
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3748..3768
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        5609..6820
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     6657..6676
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(6827..6829,6830..6832,6833..7546)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6827..6829,6830..6832,6833..7543)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6872..6893)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7133..7152)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7480..7501
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(7625..7645)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8043..8054)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8228..8461
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8533..8654
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8570..8589)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8624..8643
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8694..8829
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8849..8868)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8850..8868)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8878..8895)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8878..8894)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8887..8909)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9036..9491
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9123..9142)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9333..9354
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9517..9621
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9622..9690,9691..10482)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9840..9859)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10653..11241
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11142..11161
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11395..11412
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagcttg
      181 gccattgcat acgttgtatc catatcataa tatgtacatt tatattggct catgtccaac
      241 attaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
      301 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
      361 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
      421 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
      481 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
      541 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
      601 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
      661 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
      721 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
      781 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
      841 tttagtgaac cggggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
      901 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
      961 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     1021 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     1081 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     1141 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     1201 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     1261 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     1321 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     1381 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     1441 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     1501 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
     1561 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     1621 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     1681 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     1741 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     1801 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     1861 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     1921 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     1981 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     2041 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     2101 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     2161 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     2221 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     2281 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
     2341 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
     2401 aacggatctc gacggtatcg atctcgacac aaatggcagt attcatccac aattttaaaa
     2461 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     2521 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     2581 acagggacag cagagatcca gtttgggtcg aggattcgtg aggctccggt gcccgtcagt
     2641 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2701 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2761 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2821 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2881 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2941 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     3001 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     3061 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     3121 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     3181 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     3241 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     3301 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     3361 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3421 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3481 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3541 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3601 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3661 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3721 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3781 ttccatttca ggtgtcgtga gctagcatgg aaatgcagga tctaaccagc ccgcatagcc
     3841 gtctgagtgg tagtagtgaa tcccccagtg gccccaaact cggtaactct catataaata
     3901 gtaattccat gactcccaat ggcaccgaag ttaaaacaga gccaatgagc agcagtgaaa
     3961 cagcttcaac gacagccgac gggtctttaa acaatttctc aggttcagca attgggagca
     4021 gtagtttcag cccacgacca actcaccagt tctctccacc acagatttac ccttccaaca
     4081 gaccataccc acatattctc cctacccctt cctcacaaac tatggctgca tatgggcaaa
     4141 cacagtttac cacaggaatg caacaagcta cagcctatgc cacgtaccca cagccaggac
     4201 agccgtacgg catttcctca tatggtgcat tgtgggcagg catcaagact gaaggtggat
     4261 tgtcacagtc tcagtcacct ggacagacag gatttctcag ctatggcaca agcttcagta
     4321 cccctcaacc tggacaggca ccatacagct accagatgca aggtagcagt tttacaacat
     4381 catcaggaat atatacagga aataattcac tcacaaattc ctctggattt aatagttcac
     4441 agcaggacta tccgtcttat cccagttttg gccagggtca gtacgcacag tattataaca
     4501 gctcaccgta tccagcacat tatatgacca gcagcaacac cagcccaacg acaccatcca
     4561 ccaatgccac ttaccagctt caagaaccgc catctggcat caccagccaa gcagttacag
     4621 atcccacagc agagtacagc acaatccaca gcccatcaac acccattaaa gattcagatt
     4681 ctgatcgatt gcgtcgaggt tcagatggga aatcacgtgg acggggccga agaaacaata
     4741 atccttcacc tcccccagat tctgatcttg agagagtgtt catctgggac ttggatgaga
     4801 caatcattgt tttccactcc ttgcttactg ggtcctacgc caacagatat gggagggatc
     4861 cacccacttc agtttccctt ggactgcgaa tggaagaaat gattttcaac ttggcagaca
     4921 cacatttatt ttttaatgac ttagaagaat gtgaccaagt ccatatagat gatgtttctt
     4981 cagatgataa cggacaggac ctaagcacat ataactttgg aacagatggc tttcctgctg
     5041 cagcaaccag tgctaactta tgtttggcaa ctggtgtacg gggcggtgtg gactggatga
     5101 gaaagttggc cttccgctac agacgggtaa aagagatcta caacacctac aaaaataatg
     5161 ttggaggtct gcttggtcca gctaagaggg aagcctggct gcagttgagg gccgaaattg
     5221 aagccctgac cgactcctgg ttgacactgg ccctgaaagc actctcgctc attcactccc
     5281 ggacaaactg tgtgaatatt ttagtaacaa ctactcagct catcccagca ttggcgaaag
     5341 tcctgctgta tgggttagga attgtatttc caatagaaaa tatttacagt gcaactaaaa
     5401 taggaaaaga aagctgtttt gagagaataa ttcaaaggtt tggaagaaaa gtggtgtatg
     5461 ttgttatagg agatggtgta gaagaagaac aaggagcaaa aaagcacgcg atgcccttct
     5521 ggaggatctc cagccactcg gacctcatgg ccctgcacca tgccttggaa ctggagtacc
     5581 tgtaagtcga cggatccccc gggctgcagg aattcgcgcc gggttttggc gcctcccgcg
     5641 ggcgcccccc tcctcacggc gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct
     5701 gatccttccg cccggacgct caggacagcg gcccgctgct cataagactc ggccttagaa
     5761 ccccagtatc agcagaagga cattttagga cgggacttgg gtgactctag ggcactggtt
     5821 ttctttccag agagcggaac aggcgaggaa aagtagtccc ttctcggcga ttctgcggag
     5881 ggatctccgt ggggcggtga acgccgatga ttatataagg acgcgccggg tgtggcacag
     5941 ctagttccgt cgcagccggg atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt
     6001 cacttggtga gtagcgggct gctgggctgg ccggggcttt cgtggccgcc gggccgctcg
     6061 gtgggacgga agcgtgtgga gagaccgcca agggctgtag tctgggtccg cgagcaaggt
     6121 tgccctgaac tgggggttgg ggggagcgca gcaaaatggc ggctgttccc gagtcttgaa
     6181 tggaagacgc ttgtgaggcg ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg
     6241 cggcaagaac ccaaggtctt gaggccttcg ctaatgcggg aaagctctta ttcgggtgag
     6301 atgggctggg gcaccatctg gggaccctga cgtgaagttt gtcactgact ggagaactcg
     6361 gtttgtcgtc tgttgcgggg gcggcagtta tggcggtgcc gttgggcagt gcacccgtac
     6421 ctttgggagc gcgcgccctc gtcgtgtcgt gacgtcaccc gttctgttgg cttataatgc
     6481 agggtggggc cacctgccgg taggtgtgcg gtaggctttt ctccgtcgca ggacgcaggg
     6541 ttcgggccta gggtaggctc tcctgaatcg acaggcgccg gacctctggt gaggggaggg
     6601 ataagtgagg cgtcagtttc tttggtcggt tttatgtacc tatcttctta agtagctgaa
     6661 gctccggttt tgaactatgc gctcggggtt ggcgagtgtg ttttgtgaag ttttttaggc
     6721 accttttgaa atgtaatcat ttgggtcaat atgtaatttt cagtgttaga ctagtaaatt
     6781 gtccgctaaa ttctggccgt ttttggcttt tttgttagac ctcgagatgg tgagcaaggg
     6841 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     6901 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     6961 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     7021 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     7081 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     7141 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     7201 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     7261 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     7321 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     7381 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     7441 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     7501 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaggat atcggtaccg
     7561 catgcgttta aactcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     7621 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     7681 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     7741 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     7801 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     7861 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     7921 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     7981 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     8041 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     8101 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     8161 actttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     8221 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     8281 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     8341 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     8401 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     8461 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     8521 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     8581 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     8641 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc
     8701 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     8761 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     8821 ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact
     8881 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     8941 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     9001 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
     9061 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     9121 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     9181 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     9241 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     9301 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     9361 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     9421 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     9481 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     9541 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     9601 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     9661 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     9721 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     9781 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     9841 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     9901 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     9961 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
    10021 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
    10081 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
    10141 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
    10201 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
    10261 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
    10321 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
    10381 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
    10441 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
    10501 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
    10561 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
    10621 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
    10681 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
    10741 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
    10801 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
    10861 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
    10921 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
    10981 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
    11041 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
    11101 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
    11161 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
    11221 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
    11281 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
    11341 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
    11401 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
    11461 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
    11521 aacgcaat
//
