LOCUS       sequence-274999-        2641 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Acceptor plasmid for L0 parts. Clone L0 parts into pUAP4 with SapI, 
            lacZ blue-white screening. Release parts to assemble transcription 
            units with BsaI..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: pUAP4
FEATURES             Location/Qualifiers
     source          1..2641
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      90..133
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(137..155)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             317..976
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(383..402)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      997..1091
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      1274..1862
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1763..1782
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2016..2033
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2057..2114)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     protein_bind    2188..2209
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2224..2229,2230..2247,2248..2254)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2262..2278
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2267..2289
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2286..2302
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2286..2302
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    2315..2371
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2372..2389)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2372..2388)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2381..2403)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2598..2617)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
       61 aagctgtggc tcttcgagac cgaattccag aaatcatcct tagcgaaagc taaggatttt
      121 ttttatctga aattctgcct cgtgatacgc ctatttttat aggttaatgt catgataata
      181 atggtttctt agacgtcagg tggcactcga gttgatcggg cacgtaagag gttccaactt
      241 tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga gattttcagg
      301 agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca
      361 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca
      421 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt
      481 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aatttcgtat
      541 ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt
      601 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca
      661 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
      721 taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag
      781 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa
      841 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt
      901 ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg
      961 gcagggcggg gcgtaatttg atatcgagct cgcttggact cctgttgata gatccagtaa
     1021 tgacctcaga actccatctg gatttgttca gaacgctcgg ttgccgccgg gcgtttttta
     1081 ttggtgagaa tccaagcctc gagctgtcag accaagttta ctcatatata ctttagattg
     1141 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     1201 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     1261 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     1321 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     1381 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     1441 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     1501 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     1561 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     1621 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     1681 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     1741 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     1801 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctgtgga
     1861 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1921 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1981 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcct
     2041 gcataacgcg aagtaatctt ttcggtttta aagaaaaagg gcagggtggt gacaccttgc
     2101 ccttttttgc cggactgcag ggtctcgaag agccagctgg cacgacaggt ttcccgactg
     2161 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     2221 ggctttacac tatatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     2281 tcacacagga aacagctatg accatgatta cgccaagctt gcatgcctgc aggtcgactc
     2341 tagaggatcc ccgggtaccg agctcgaatt cactggccgt cgttttacaa cgtcgtgact
     2401 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     2461 ggcgtaatag cgaagaggcc tgcgccgatc gcccttccca acagttgcgc agcctgaatg
     2521 gcggatgagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     2581 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     2641 c
//
