LOCUS       sequence-275001-        2856 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Confers plant resistance to kanamycin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: CDS_nptII
FEATURES             Location/Qualifiers
     source          1..2856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     22..41
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     275..292
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(316..373)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     CDS             complement(391..1188)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(502..521)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1112..1131
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     terminator      1205..1248
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(1252..1270)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             1432..2091
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1498..1517)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2112..2206
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      join(2389..2856,1..121)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
       61 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctgtggaa
      121 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
      181 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
      241 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcctg
      301 cataacgcga agtaatcttt tcggttttaa agaaaaaggg cagggtggtg acaccttgcc
      361 cttttttgcc ggactgcagg gtctcgaagc tcagaagaac tcgtcaagaa ggcgatagaa
      421 ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca
      481 ttcgccgcca agctcctcag caatatcacg ggtagccaac gctatgtcct gatagcggtc
      541 cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat
      601 attcggcaag caggcatcgc catgtgtcac gacgagatcc tcgccgtcgg gcatgcgcgc
      661 cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcctcgt ccagatcatc
      721 ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg
      781 gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat
      841 gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc
      901 gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg
      961 aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcctggagtt cattcagggc
     1021 accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccgaaacac
     1081 ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac
     1141 ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atccccattt gagaccgaat
     1201 tccagaaatc atccttagcg aaagctaagg atttttttta tctgaaattc tgcctcgtga
     1261 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     1321 ctcgagttga tcgggcacgt aagaggttcc aactttcacc ataatgaaat aagatcacta
     1381 ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa aatggagaaa
     1441 aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag
     1501 gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc
     1561 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt
     1621 gcccgcctga tgaatgctca tccggaattt cgtatggcaa tgaaagacgg tgagctggtg
     1681 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca
     1741 tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat
     1801 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt
     1861 ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg
     1921 gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg
     1981 ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga
     2041 atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta atttgatatc
     2101 gagctcgctt ggactcctgt tgatagatcc agtaatgacc tcagaactcc atctggattt
     2161 gttcagaacg ctcggttgcc gccgggcgtt ttttattggt gagaatccaa gcctcgagct
     2221 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     2281 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     2341 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     2401 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     2461 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     2521 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     2581 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     2641 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     2701 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     2761 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     2821 caggtatccg gtaagcggca gggtcggaac aggaga
//
