LOCUS       sequence-275005-        4095 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Confers plant resistance to chlorsulfuron.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: CDS_mALS
FEATURES             Location/Qualifiers
     source          1..4095
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      421..515
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      698..1286
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1187..1206
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1440..1457
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1481..1538)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      3609..3652
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(3656..3674)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             join(3836..4095,1..400)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3902..3921)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag
       61 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta
      121 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc
      181 caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt
      241 cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct
      301 ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga
      361 attacaacag tactgcgatg agtggcaggg cggggcgtaa tttgatatcg agctcgcttg
      421 gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc
      481 tcggttgccg ccgggcgttt tttattggtg agaatccaag cctcgagctg tcagaccaag
      541 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
      601 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
      661 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
      721 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
      781 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
      841 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
      901 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
      961 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     1021 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     1081 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     1141 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     1201 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     1261 cgtcaggggg gcggagcctg tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     1321 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     1381 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     1441 gcgagtcagt gagcgaggaa gcctgcataa cgcgaagtaa tcttttcggt tttaaagaaa
     1501 aagggcaggg tggtgacacc ttgccctttt ttgccggact gcagggtctc gaatggcgtc
     1561 aattagtgga tgcgcagctg tgcctttgag catagcgagc ctggagaagg tgcagccatc
     1621 ggcaaggtct tgttgcaagg gagctaaaga gctaaaggtg aggagtggtt tcttctcggg
     1681 agccgctctg aggggacatg catacaacac ctctcggaag actgagcgtt cactggaagt
     1741 gaaagcccaa gctgttagcg agaagatcca gtctgcagtc gatgtgagtg gaccacggct
     1801 tagcagcggg aagtcactcg ttgcaccaac cccgtccgac tttcagtctc gctacaagga
     1861 tgacgagccc cgcaaaggag ccgacatttt ggtggaggct cttgagcgag aagggattag
     1921 aacggtgttc gcttatcccg gcggagcgtc tatggagatt caccaggctc ttacacgttc
     1981 cagcagcatc cgcaacattc tctgtcgcca cgagcagggg gagattttcg ctgctgaagg
     2041 ttatgctaaa tcgtctggac gagtaggggt ttgtatagcc acatccggtc ctggagcgac
     2101 caatcttgtt accgggctcg ctgatgccct cttggacagt gtgccactag tggccattac
     2161 aggtcaagta tccagtagga tgattggtac cgatgctttt caagaaactc caattgtgga
     2221 ggttacgaga tcgatcacca agcacaatta ccttgttatg aacattgatg acatccctcg
     2281 cgtaatcagg gaggctttct ttttggcagc ttcaggtagg ccgggcccag tcttggtaga
     2341 tattcccaag gacgtgcaac agcaaatggc tattccagat tggtcgcagc caatgcaact
     2401 gaagggctac ttacaacgac tgcctcggcc accggaacca acgttaatcg agcagattat
     2461 aagactcatt tctagttcta agaagcctgt agtgtattct ggaggaggtt gcaatcacgc
     2521 cagcgaagag ttgagggaat ttgtcgagct taccggaatt cctatcacat cgactttaat
     2581 gggtttggga gcgtatcccg agtctgatcc attaagtttg aaaatgcttg gaatgcatgg
     2641 aactgtttac gccaactacg ccgtggacca agcagatctg ttgttggcat ttggagtgcg
     2701 tttcgacgat cgagtgactg gtaaactgga gacttttgct agcaggtcca gcatcgttca
     2761 tatcgatatt gaccctgctg aaattggaaa gaacaaacag ccccatgtgt ccatgtgtgc
     2821 tgatgtgaaa cctgcgctaa gtgaaatcaa tgccctcttg aagagtcaaa agaggacttt
     2881 cgacttctcc aactgggtgg cagaactaga agaacaaaag cagaaatggc caatgaagtt
     2941 tcccgtattc gaggatgcca ttgctcctca atctgccata cagacgctgt acgaactcac
     3001 tggtggaaat gccatcatat ctactggtgt tggccaacat caaatgtggg cagcccagtg
     3061 gttcaagtac aacgaaccgc ggcgttggtt gacatctgga ggtcttggag ccatgggatt
     3121 cggacttcct tctgctttgg gggctgccgc aactcaacca gatattcctg tgatcgacat
     3181 cgatggcgat ggtagctttg tgatgaacgt acaggagttg gctacaattt ttgtggagaa
     3241 gttacccgtg aagatcatga tcttaaacaa ccaacatcta ggaatggtcg tacaattgga
     3301 ggatagattc tacaaggcca acagagcaca tacttactta ggtgatccag atcacgagga
     3361 gcagatctac cccgacttct gtacagttgc agacggtttc aaggtgccaa atgcgcgtgt
     3421 ggtgaagaag gatgaactaa gagcagcaat tcaaactatg ctagattctc ccggtcctta
     3481 tttactggat gtgatagttc cccaccagga gcacgttctg cctatgattc ctgggggagc
     3541 aagttttaag gagattatca cagagggtga tggaaggact atatactaag ctttgagacc
     3601 gaattccaga aatcatcctt agcgaaagct aaggattttt tttatctgaa attctgcctc
     3661 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     3721 ggcactcgag ttgatcgggc acgtaagagg ttccaacttt caccataatg aaataagatc
     3781 actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga
     3841 gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt
     3901 tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac
     3961 ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat
     4021 tcttgcccgc ctgatgaatg ctcatccgga atttcgtatg gcaatgaaag acggtgagct
     4081 ggtgatatgg gatag
//
