LOCUS       sequence-275010-        3084 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Confers plant resistance to hygromycin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: CDS_hptII
FEATURES             Location/Qualifiers
     source          1..3084
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     66..84
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(88..131)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     CDS             148..1173
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     terminator      1191..1248
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1272..1289)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1443..2031)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1523..1542)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2214..2308)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2329..2988)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2903..2922
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cccgatcaac tcgagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
       61 ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta gctttcgcta
      121 aggatgattt ctggaattcg gtctcgaatg aaaaagcctg aactcaccgc gacgtctgtc
      181 gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc
      241 gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat
      301 agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg
      361 ctcccgattc cggaagtgct tgacattggg gagtttagcg agagcctgac ctattgcatc
      421 tcccgccgtt cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt
      481 ctacaaccgg tcgcggaggc tatggatgcg atcgctgcgg ccgatcttag ccagacgagc
      541 gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata
      601 tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt
      661 gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc
      721 cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata
      781 acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac
      841 atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg
      901 aggcatccgg agcttgcagg atcgccacga ctccgggcgt atatgctccg cattggtctt
      961 gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt
     1021 cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc
     1081 agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga
     1141 cgccccagca ctcgtccgag ggcaaagaaa taggctttga gaccctgcag tccggcaaaa
     1201 aagggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt
     1261 atgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     1321 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     1381 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     1441 tttttccaca ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     1501 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     1561 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     1621 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     1681 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     1741 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     1801 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     1861 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     1921 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     1981 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     2041 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     2101 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     2161 aaatcaatct aaagtatata tgagtaaact tggtctgaca gctcgaggct tggattctca
     2221 ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag
     2281 gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc
     2341 ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc
     2401 acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata
     2461 atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc
     2521 aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc
     2581 tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag
     2641 aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc
     2701 atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat
     2761 tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg
     2821 ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac
     2881 ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg
     2941 ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt
     3001 agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg
     3061 gtgaaagttg gaacctctta cgtg
//
