LOCUS       sequence-275013-        3711 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Firefly luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 3711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: CDS_LUC
FEATURES             Location/Qualifiers
     source          1..3711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     55..73
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(77..120)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     CDS             137..1789
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(203..221)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      1807..1864
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     rep_origin      complement(2059..2647)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2139..2158)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2830..2924)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2945..3604)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3519..3538
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cgagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
       61 cgtatcacga ggcagaattt cagataaaaa aaatccttag ctttcgctaa ggatgatttc
      121 tggaattcgg tctcaaatgg aagacgccaa aaacataaag aaaggcccgg cgccattcta
      181 tccgctggaa gatggaaccg ctggagagca actgcataag gctatgaaga gatacgccct
      241 ggttcctgga acaattgctt ttacagatgc acatatcgag gtggacatca cttacgctga
      301 gtacttcgaa atgtccgttc ggttggcaga agctatgaaa cgatatgggc tgaatacaaa
      361 tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc
      421 gttatttatc ggagttgcag ttgcgcccgc gaacgacatt tataatgaac gtgaattgct
      481 caacagtatg ggcatttcgc agcctaccgt ggtgttcgtt tccaaaaagg ggttgcaaaa
      541 aattttgaac gtgcaaaaaa agctcccaat catccaaaaa attattatca tggattctaa
      601 aacggattac cagggatttc agtcgatgta cacgttcgtc acatctcatc tacctcccgg
      661 ttttaatgaa tacgattttg tgccagagtc cttcgatagg gacaagacaa ttgcactgat
      721 catgaactcc tctggatcta ctggtctgcc taaaggtgtc gctctgcctc atagaactgc
      781 ctgcgtgaga ttctcgcatg ccagagatcc tatttttggc aatcaaatca ttccggatac
      841 tgcgatttta agtgttgttc cattccatca cggttttgga atgtttacta cactcggata
      901 tttgatatgt ggatttcgag tcgtcttaat gtatagattt gaagaggagc tgtttctgag
      961 gagccttcag gattacaaga ttcaaagtgc gctgctggtg ccaaccctat tctccttctt
     1021 cgccaaaagc actctgattg acaaatacga tttatctaat ttacacgaaa ttgcttctgg
     1081 tggcgctccc ctctctaagg aagtcgggga agcggttgcc aagaggttcc atctgccagg
     1141 tatcaggcaa ggatatgggc tcactgagac tacatcagct attctgatta cacccgaggg
     1201 ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga aggttgtgga
     1261 tctggatacc gggaaaacgc tgggcgttaa tcaaagaggc gaactgtgtg tgagaggtcc
     1321 tatgattatg tccggttatg taaacaatcc ggaagcgacc aacgccttga ttgacaagga
     1381 tggatggcta cattctggag acatagctta ctgggacgaa gacgaacact tcttcatcgt
     1441 tgaccgcctg aagtctctga ttaagtacaa aggctatcag gtggctcccg ctgaattgga
     1501 atccatcttg ctccaacacc ccaacatctt cgacgcaggt gtcgcaggtc ttcccgacga
     1561 tgacgccggt gaacttcccg ccgccgttgt tgttttggag cacggaaaga cgatgacgga
     1621 aaaagagatc gtggattacg tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg
     1681 agttgtgttt gtggacgaag taccgaaagg tcttaccgga aaactcgacg caagaaaaat
     1741 cagagagatc ctcataaagg ccaagaaggg cggaaagatc gccgtgtaag cttcgagacc
     1801 ctgcagtccg gcaaaaaagg gcaaggtgtc accaccctgc cctttttctt taaaaccgaa
     1861 aagattactt cgcgttatgc aggcttcctc gctcactgac tcgcggcgct cggtcgttcg
     1921 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     1981 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     2041 ggccgcgttg ctggcgtttt tccacaggct ccgcccccct gacgagcatc acaaaaatcg
     2101 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2161 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     2221 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     2281 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     2341 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     2401 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2461 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     2521 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     2581 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     2641 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     2701 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     2761 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagctc
     2821 gaggcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc
     2881 cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat
     2941 caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc
     3001 cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct
     3061 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat
     3121 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca
     3181 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
     3241 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa
     3301 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
     3361 gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa
     3421 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
     3481 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa
     3541 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct
     3601 ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg
     3661 atcttatttc attatggtga aagttggaac ctcttacgtg cccgatcaac t
//
