LOCUS       sequence-275018-        2756 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FP for N-term fusion with a CTAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: CDS12_mScarlet-I
FEATURES             Location/Qualifiers
     source          1..2756
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     30..48
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(52..95)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     CDS             112..807
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(245..263)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     513..532
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      827..884
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(908..925)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1079..1667)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1159..1178)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1850..1944)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1965..2624)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2539..2558
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ccattattat catgacatta acctataaaa ataggcgtat cacgaggcag aatttcagat
       61 aaaaaaaatc cttagctttc gctaaggatg atttctggaa ttcggtctca aatggtgagc
      121 aagggcgagg cagtgatcaa ggagttcatg cggttcaagg tgcacatgga gggctccatg
      181 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
      241 accgccaagc tgaaggtgac caagggtggc cccctgccct tctcctggga catcctgtcc
      301 cctcagttca tgtacggctc cagggccttc atcaagcacc ccgccgacat ccccgactac
      361 tataagcagt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
      421 ggcgccgtga ccgtgaccca ggacacctcc ctggaggacg gcaccctgat ctacaaggtg
      481 aagctccgcg gcaccaactt ccctcctgac ggccccgtaa tgcagaagaa gacaatgggc
      541 tgggaagcgt ccaccgagcg gttgtacccc gaggacggcg tgctgaaggg cgacattaag
      601 atggccctgc gcctgaagga cggcggccgc tacctggcgg acttcaagac cacctacaag
      661 gccaagaagc ccgtgcagat gcccggcgcc tacaacgtcg accgcaagtt ggacatcacc
      721 tcccacaacg aggactacac cgtggtggaa cagtacgaac gctccgaggg ccgccactcc
      781 accggcggca tggacgagct gtacaaggct tcgcgagacc ctgcagtccg gcaaaaaagg
      841 gcaaggtgtc accaccctgc cctttttctt taaaaccgaa aagattactt cgcgttatgc
      901 aggcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
      961 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     1021 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     1081 tccacaggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     1141 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1201 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1261 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1321 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1381 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1441 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     1501 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1561 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     1621 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1681 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     1741 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     1801 caatctaaag tatatatgag taaacttggt ctgacagctc gaggcttgga ttctcaccaa
     1861 taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca
     1921 ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc
     1981 cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa
     2041 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat
     2101 ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa
     2161 ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta
     2221 gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac
     2281 tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg
     2341 aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc
     2401 atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa
     2461 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc
     2521 tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat
     2581 tgggatatat caacggtggt atatccagtg atttttttct ccattttagc ttccttagct
     2641 cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga
     2701 aagttggaac ctcttacgtg cccgatcaac tcgagtgcca cctgacgtct aagaaa
//
