LOCUS       sequence-275019-        2789 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FP for N-term fusion with a CTAG without linker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: CDS12_mScarletI-linker
FEATURES             Location/Qualifiers
     source          1..2789
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     74..92
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(96..139)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     CDS             156..851
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(289..307)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     557..576
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      904..961
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(985..1002)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1156..1744)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1236..1255)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1927..2021)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2042..2701)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2616..2635
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cttacgtgcc cgatcaactc gagtgccacc tgacgtctaa gaaaccatta ttatcatgac
       61 attaacctat aaaaataggc gtatcacgag gcagaatttc agataaaaaa aatccttagc
      121 tttcgctaag gatgatttct ggaattcggt ctcgaatggt gagcaagggc gaggcagtga
      181 tcaaggagtt catgcggttc aaggtgcaca tggagggctc catgaacggc cacgagttcg
      241 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
      301 tgaccaaggg tggccccctg cccttctcct gggacatcct gtcccctcag ttcatgtacg
      361 gctccagggc cttcatcaag caccccgccg acatccccga ctactataag cagtccttcc
      421 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgcc gtgaccgtga
      481 cccaggacac ctccctggag gacggcaccc tgatctacaa ggtgaagctc cgcggcacca
      541 acttccctcc tgacggcccc gtaatgcaga agaagacaat gggctgggaa gcgtccaccg
      601 agcggttgta ccccgaggac ggcgtgctga agggcgacat taagatggcc ctgcgcctga
      661 aggacggcgg ccgctacctg gcggacttca agaccaccta caaggccaag aagcccgtgc
      721 agatgcccgg cgcctacaac gtcgaccgca agttggacat cacctcccac aacgaggact
      781 acaccgtggt ggaacagtac gaacgctccg agggccgcca ctccaccggc ggcatggacg
      841 agctgtacaa gtccggagct gcggccgctg ccgctgcggc agcggcttcg tgagaccctg
      901 cagtccggca aaaaagggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag
      961 attacttcgc gttatgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     1021 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     1081 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     1141 cgcgttgctg gcgtttttcc acaggctccg cccccctgac gagcatcaca aaaatcgacg
     1201 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     1261 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     1321 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     1381 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     1441 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     1501 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     1561 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     1621 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     1681 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1741 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     1801 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     1861 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagctcgag
     1921 gcttggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag
     1981 atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa
     2041 attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
     2101 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
     2161 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
     2221 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat
     2281 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
     2341 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
     2401 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
     2461 caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt
     2521 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
     2581 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
     2641 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
     2701 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
     2761 ttatttcatt atggtgaaag ttggaacct
//
