LOCUS       sequence-275020-        2777 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FP for N-term fusion with a CTAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: CDS12_mTurquoise2
FEATURES             Location/Qualifiers
     source          1..2777
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     83..101
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(105..148)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     CDS             join(165..167,168..170,171..881)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(210..231)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(471..490)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     818..839
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      901..958
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(982..999)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1153..1741)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1233..1252)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1924..2018)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2039..2698)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2613..2632
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ttggaacctc ttacgtgccc gatcaactcg agtgccacct gacgtctaag aaaccattat
       61 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
      121 atccttagct ttcgctaagg atgatttctg gaattcggtc tcaaatggtg agcaagggcg
      181 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
      241 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
      301 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctgt
      361 cctggggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca
      421 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
      481 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
      541 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
      601 actttagcga caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact
      661 tcaagatccg ccacaacatc gaggacggcg gcgtgcagct cgccgaccac taccagcaga
      721 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
      781 ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
      841 ccgccgccgg gatcactctc ggcatggacg agctgtacaa ggcttcgcga gaccctgcag
      901 tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt
      961 acttcgcgtt atgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     1021 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     1081 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     1141 gttgctggcg tttttccaca ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     1201 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     1261 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     1321 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     1381 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     1441 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     1501 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     1561 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     1621 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     1681 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     1741 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     1801 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     1861 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gctcgaggct
     1921 tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg
     1981 gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt
     2041 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat
     2101 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc
     2161 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca
     2221 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct
     2281 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat
     2341 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt
     2401 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac
     2461 cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa
     2521 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat
     2581 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt
     2641 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt
     2701 tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta
     2761 tttcattatg gtgaaag
//
