LOCUS       sequence-275021-        2810 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FP for N-term fusion with a CTAG without linker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: CDS12_mTurquoise2-linker
FEATURES             Location/Qualifiers
     source          1..2810
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     113..131
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(135..178)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     CDS             join(195..197,198..200,201..911)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(240..261)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(501..520)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     848..869
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      964..1021
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1045..1062)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1216..1804)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1296..1315)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1987..2081)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2102..2761)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2676..2695
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccc gatcaactcg
       61 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
      121 tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg atgatttctg
      181 gaattcggtc tcaaatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
      241 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
      301 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
      361 tgccctggcc caccctcgtg accaccctgt cctggggcgt gcagtgcttc gcccgctacc
      421 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
      481 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
      541 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
      601 acatcctggg gcacaagctg gagtacaact actttagcga caacgtctat atcaccgccg
      661 acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggcg
      721 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
      781 tgcccgacaa ccactacctg agcacccagt ccaagctgag caaagacccc aacgagaagc
      841 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
      901 agctgtacaa gtccggagct gcggccgctg ccgctgcggc agcggcttcg cgagaccctg
      961 cagtccggca aaaaagggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag
     1021 attacttcgc gttatgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     1081 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     1141 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     1201 cgcgttgctg gcgtttttcc acaggctccg cccccctgac gagcatcaca aaaatcgacg
     1261 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     1321 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     1381 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     1441 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     1501 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     1561 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     1621 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     1681 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     1741 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1801 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     1861 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     1921 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagctcgag
     1981 gcttggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag
     2041 atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa
     2101 attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
     2161 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
     2221 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
     2281 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat
     2341 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
     2401 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
     2461 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
     2521 caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt
     2581 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
     2641 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
     2701 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
     2761 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc
//
