LOCUS       sequence-275023-        2810 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FP for N-term fusion with a CTAG without linker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: CDS12_mVenus-linker
FEATURES             Location/Qualifiers
     source          1..2810
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     304..323
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     557..574
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(598..655)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     CDS             complement(join(708..1418,1419..1421,1422..1424))
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(750..771)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1099..1118
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1358..1379
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     terminator      1441..1484
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(1488..1506)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             1668..2327
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1734..1753)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2348..2442
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      join(2625..2810,1..403)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
       61 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
      121 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
      181 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
      241 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
      301 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
      361 tcgatttttg tgatgctcgt caggggggcg gagcctgtgg aaaaacgcca gcaacgcggc
      421 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
      481 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
      541 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcc tgcataacgc gaagtaatct
      601 tttcggtttt aaagaaaaag ggcagggtgg tgacaccttg cccttttttg ccggactgca
      661 gggtctcgcg aagccgctgc cgcagcggca gcggccgcag ctccggactt gtacagctcg
      721 tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc
      781 ttctcgttgg ggtctttgct cagcttggac tggtagctca ggtagtggtt gtcgggcagc
      841 agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgccg
      901 ccgtcctcga tgttgtggcg gatcttgaag ttggccttga tgccgttctt ctgcttgtcg
      961 gcggtgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc caggatgttg
     1021 ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg
     1081 aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc
     1141 tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg
     1201 tagcgggcga agcactgcag gccgtagccc agggtggtca cgagggtggg ccagggcacg
     1261 ggcagcttgc cggtggtgca gatcagcttc agggtcagct tgccgtaggt ggcatcgccc
     1321 tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg
     1381 atgggcacca ccccggtgaa cagctcctcg cccttgctca ccatttgaga ccgaattcca
     1441 gaaatcatcc ttagcgaaag ctaaggattt tttttatctg aaattctgcc tcgtgatacg
     1501 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcactcg
     1561 agttgatcgg gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg
     1621 gcgtattttt tgagttatcg agattttcag gagctaagga agctaaaatg gagaaaaaaa
     1681 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat
     1741 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt
     1801 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc
     1861 gcctgatgaa tgctcatccg gaatttcgta tggcaatgaa agacggtgag ctggtgatat
     1921 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc
     1981 tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg
     2041 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg
     2101 tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca
     2161 acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga
     2221 tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc
     2281 ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt gatatcgagc
     2341 tcgcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
     2401 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaagcct cgagctgtca
     2461 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     2521 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     2581 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     2641 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     2701 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     2761 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
//
