LOCUS       sequence-275024-        2360 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Chloroplast transit peptide from MpSIG2. For N-term fusion with a 
            CTAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: CDS12_TP-MpSIG2
FEATURES             Location/Qualifiers
     source          1..2360
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      289..346
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(370..387)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(541..1129)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(621..640)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1312..1406)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1427..2086)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2001..2020
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2248..2266
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2270..2313)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gcttgcacga ctcgatcacc tgctcgccgc atgcttcgtc ccgtgatggc tcgtccggtg
       61 ttgtgaaaag cttgaggcga agttctggga ccagcaggaa gcgagttact ctcaagcaac
      121 agagagcttt tggacctaat tcttctcggt ttagcgtaga aactacttgt gcaccttcga
      181 gcatttctta cagcccctgt cgaaggccat ccaggttgtc ggcctttgat cgaattcaaa
      241 atttgcctgt cactgctgca tctgccgggg cttcgcgaga ccctgcagtc cggcaaaaaa
      301 gggcaaggtg tcaccaccct gccctttttc tttaaaaccg aaaagattac ttcgcgttat
      361 gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
      421 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
      481 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
      541 tttccacagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      601 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      661 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      721 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      781 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      841 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      901 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      961 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     1021 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1081 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1141 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     1201 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     1261 atcaatctaa agtatatatg agtaaacttg gtctgacagc tcgaggcttg gattctcacc
     1321 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt
     1381 cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct
     1441 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac
     1501 aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
     1561 atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa
     1621 aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt
     1681 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
     1741 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat
     1801 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg
     1861 ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat
     1921 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg
     1981 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc
     2041 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag
     2101 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt
     2161 gaaagttgga acctcttacg tgcccgatca actcgagtgc cacctgacgt ctaagaaacc
     2221 attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa
     2281 aaaaaatcct tagctttcgc taaggatgat ttctggaatt cggtctcaaa tggcggttgt
     2341 tgctacgagc gagctgctgc
//
