LOCUS       sequence-275025-        2126 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CDS12 with ER targeting peptide to fuse with CTAG containing HDEL ER
            retention signal at the 3'end.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: CDS12-MpER-Targ
FEATURES             Location/Qualifiers
     source          1..2126
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     393..412
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     646..663
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(687..744)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      846..889
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(893..911)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             1073..1732
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1139..1158)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1753..1847
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      join(2030..2126,1..492)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
       61 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
      121 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
      181 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
      241 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
      301 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
      361 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
      421 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
      481 agcctgtgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
      541 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
      601 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
      661 gaggaagcct gcataacgcg aagtaatctt ttcggtttta aagaaaaagg gcagggtggt
      721 gacaccttgc ccttttttgc cggactgcag ggtctcgcga agcttctgct gcgcaatgtg
      781 cagccacaat ggtgaggacc aacaccccga ccacagctct cgaagccatt tgagaccgaa
      841 ttccagaaat catccttagc gaaagctaag gatttttttt atctgaaatt ctgcctcgtg
      901 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
      961 actcgagttg atcgggcacg taagaggttc caactttcac cataatgaaa taagatcact
     1021 accgggcgta ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatggagaa
     1081 aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga
     1141 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc
     1201 ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct
     1261 tgcccgcctg atgaatgctc atccggaatt tcgtatggca atgaaagacg gtgagctggt
     1321 gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc
     1381 atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga
     1441 tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt
     1501 tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat
     1561 ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt
     1621 gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag
     1681 aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt aatttgatat
     1741 cgagctcgct tggactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt
     1801 tgttcagaac gctcggttgc cgccgggcgt tttttattgg tgagaatcca agcctcgagc
     1861 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     1921 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     1981 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     2041 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     2101 gtttgccgga tcaagagcta ccaact
//
