LOCUS       sequence-275026-         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Split Fluorescent Reporter for ER Retrograde Trafficking.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Luong P, Li Q, Chen PF, Wrighton PJ, Chang D, Dwyer S, Bayer MT, 
            Snapper SB, Hansen SH, Thiagarajah JT, Goessling W, Lencer WI
  TITLE     A quantitative single-cell assay for retrograde membrane traffic 
            enables rapid detection of defects in cellular organization.
  JOURNAL   Mol Biol Cell. 2019 Nov 27:mbcE19070375. doi: 
            10.1091/mbc.E19-07-0375.
  PUBMED    31774722
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2019 Nov 27:mbcE19070375. doi: 10.1091/mbc.E19-07-0375."
COMMENT     Sequence Label: ER-HA-mNG21-10
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             61..90
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      352..361
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atgggatgga gctgtatcat cctcttcttg gtagcaacag ctacaggcgc gcactccgga
       61 gaacagaagc tgatctcaga ggaggacctg ggaggtatgg tgagcaaggg tgaggaggat
      121 aacatggcct ctctcccagc gactcatgag ttacacatct ttggctccat caacggtgtg
      181 gactttgaca tggtgggtca gggtaccggc aatccaaatg atggttatga ggagttaaac
      241 ctgaagtcca ccaagggtga cctccagttc tccccctgga ttctggtccc tcatatcggg
      301 tatggcttcc atcagtacct gccctaccct gacgggatgt cgcctttcca ggccgccatg
      361 gtagatggct ccggatacca agtccatcgc acaatgcagt ttgaagatgg tgcctccctt
      421 actgttaact accgctacac ctacgaggga agccacatca aaggagaggc ccaggtgatg
      481 gggactggtt tccctgctga cggtcctgtg atgaccaaca cgctgaccgc tgcggactgg
      541 tgcatgtcga agaagactta ccccaacgac aaaaccatca tcagtacctt taagtggagt
      601 tacaccactg taaatggcaa acgctaccgg agcactgcgc ggaccaccta cacctttgcc
      661 aagccaatgg cggctaacta tctgaagaac cagccgatgt acgtgttccg taagacggag
      721 ctcaagcact ccatgggaga gaaggatgaa ctg
//
