LOCUS       sequence-275032-        2280 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasma Membrane targeting protein for C-term fusion with CDS12 
            without linker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: CTAG_linker-Lti6b
FEATURES             Location/Qualifiers
     source          1..2280
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      239..333
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      516..1104
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1005..1024
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1258..1275
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1299..1356)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      1612..1655
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(1659..1677)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             join(1839..2280,1..218)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1905..1924)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
       61 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg
      121 cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat
      181 tacaacagta ctgcgatgag tggcagggcg gggcgtaatt tgatatcgag ctcgcttgga
      241 ctcctgttga tagatccagt aatgacctca gaactccatc tggatttgtt cagaacgctc
      301 ggttgccgcc gggcgttttt tattggtgag aatccaagcc tcgagctgtc agaccaagtt
      361 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
      421 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
      481 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
      541 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
      601 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
      661 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
      721 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
      781 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
      841 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
      901 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
      961 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     1021 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     1081 tcaggggggc ggagcctgtg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     1141 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     1201 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     1261 gagtcagtga gcgaggaagc ctgcataacg cgaagtaatc ttttcggttt taaagaaaaa
     1321 gggcagggtg gtgacacctt gccctttttt gccggactgc agggtctcgt tcgggagctg
     1381 cggcagctgc cgctgcggca gcggccgaat tcaagcgctt gaaaatgagt acagccactt
     1441 tcgtagagat tattcttgct atcatcttgc ctcctctcgg cgtctttctc aaatttggtt
     1501 gcaaggttga gttttggata tgtttgattt tgacgctgtt tggttatctt cccggaatcc
     1561 tttacgctct ttatatcatc accttttgat gagctttgag accgaattcc agaaatcatc
     1621 cttagcgaaa gctaaggatt ttttttatct gaaattctgc ctcgtgatac gcctattttt
     1681 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactc gagttgatcg
     1741 ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt
     1801 ttgagttatc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
     1861 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
     1921 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
     1981 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
     2041 atgctcatcc ggaatttcgt atggcaatga aagacggtga gctggtgata tgggatagtg
     2101 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
     2161 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
     2221 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca
//
