LOCUS       sequence-275038-        2313 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear targeting peptide for C-term fusion with CDS12 without 
            linker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: CTAG_linker-N7
FEATURES             Location/Qualifiers
     source          1..2313
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      13..601
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     502..521
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     755..772
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(796..853)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      1142..1185
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(1189..1207)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             1369..2028
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1435..1454)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2049..2143
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
       61 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
      121 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
      181 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
      241 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
      301 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
      361 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
      421 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
      481 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
      541 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctgtggaa
      601 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
      661 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
      721 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcctg
      781 cataacgcga agtaatcttt tcggttttaa agaaaaaggg cagggtggtg acaccttgcc
      841 cttttttgcc ggactgcagg gtctcgttcg gctgcagcgg ccgaattcaa gcgtgaagaa
      901 caagcaagga aagctaaggt gaacaatgag aaaaagacgg aaatagtgaa accagagagt
      961 tgtagcaatg aaggagatgt caaggatctg aaaagaaagg actctgagga tggaaacgag
     1021 ggtgaggaag aagaagcttc ttcgaaaccg aaaaagccaa aagttgctct ttctcatctt
     1081 caggacattg acgacacaga agctgatcaa gaagaagagt aagctttgag accgaattcc
     1141 agaaatcatc cttagcgaaa gctaaggatt ttttttatct gaaattctgc ctcgtgatac
     1201 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactc
     1261 gagttgatcg ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg
     1321 ggcgtatttt ttgagttatc gagattttca ggagctaagg aagctaaaat ggagaaaaaa
     1381 atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca
     1441 tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt
     1501 ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc
     1561 cgcctgatga atgctcatcc ggaatttcgt atggcaatga aagacggtga gctggtgata
     1621 tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg
     1681 ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg
     1741 gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc
     1801 gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac
     1861 aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg
     1921 atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg
     1981 cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaatt tgatatcgag
     2041 ctcgcttgga ctcctgttga tagatccagt aatgacctca gaactccatc tggatttgtt
     2101 cagaacgctc ggttgccgcc gggcgttttt tattggtgag aatccaagcc tcgagctgtc
     2161 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     2221 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     2281 gttccactga gcgtcagacc ccgtagaaaa gat
//
