LOCUS       sequence-275039-        4788 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  5UTR for cloning with PROM_MpNOP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 4788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: 5UTR_MpNOP1
FEATURES             Location/Qualifiers
     source          1..4788
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     49..67
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(71..114)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      2878..2935
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2959..2976)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3130..3718)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3210..3229)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3901..3995)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(4016..4675)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4590..4609
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
       61 acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat
      121 tcggtctcgt actggcttga accttgtgac ttggctgcct agctgtccat tttgtccgac
      181 tggttctcct gggccttgat ggtgcacaca atggagtccg tgcgagtcag tgggcgggca
      241 gacacagcta gagcccccgc actgtgagca gccttcaagg gaaggccagg ggccgagaga
      301 aatgcgctcc aacggcccgg ccccgatggg atggcatggc atgggaggag gacgagcagc
      361 agctggttcg cctggaggtg aagaagagta gaggtggagt ttagtgagtc cagttcacag
      421 aggagggcgc cggaaaagac tcctgactca tgactgggat cagacacatg atggcggatg
      481 acctttctgc cttcctgcgc atgactctga gctcaagtct catcttgtag tcgaggtgag
      541 cgaatgactg tgtatgctcg tagtttacat gtttgttgct cggccttcgt tctgcctcaa
      601 gttcagcagc aagctcagtc ctgggctcag cttcccccgt ctcctctctc tctctatctc
      661 cctccctcgt ttctatttcc tctctcgtgg tcttcggtcc cgttcggtct gttctgtagg
      721 gtggatgcta ttatggtctg ttgaagggca tcatgggatg ggttggattg ggacgagcag
      781 ctggaatgga gtgagtctga agctcttatt cgggctcgcc tcccgctctt ggagctgcaa
      841 acgaagccgc gcagcgtcgt gcgtcagctc cacaagtctg ggacggaggg attgatgcat
      901 atttactaag tttacagccc cttcggttgg atcctcgtcg gcctctgtca tcattcgttc
      961 tgttcgttcg tccgaggcgg tgctgtgaaa gaacgggtcg tcgctgggtt gggttgggtt
     1021 gactttcggg ccaatttgcg agccccgaac cggggacgga cctggctggg ccttgattct
     1081 tcaattcggc cttgaccacg tcacgccctc ccgcttgtcc ttgctcgctg cctcgacccc
     1141 gcccggacag ctagaacgag attcctggag cccgcaggtg gagcttatct cctcctcccc
     1201 gcgcactccg aattttcctc gcctctgctg cctcggactg tcgctcgtcc cgcccgcgcc
     1261 caggagacga ggtcccgtgc catctctcgt ctgtgtctct ctcggtcctg ttgtgctgct
     1321 ctcttggcca ctggaggcga gcaagcgagc aagcgagaaa ggaaggaagt aaggataggt
     1381 gcctgacggc ggacggagcc gcagctggag ctggggtcgt tcttcttctt tgcgtgtagg
     1441 gagacgacga agtgccggca ttgtagagat cagcggtgtc acactcacgc ctgtgcgaat
     1501 agtatcggaa tgaaataggt ttatgcttga gctctggggc gagggggctt tctgcattga
     1561 gttgagcgtc ggcccgagaa ggcggggagg caggcagtcc accgaggtcg gccatagctt
     1621 tccttcccaa cctcgtatgt gctcgccgga atgaaggaag agaatgatca tgatactgcc
     1681 gagatgttgc aagagcccgt ggtgggatca gagcatggag tttgattttt ggtaagcagt
     1741 atgagttatg gttcagagag tggaaggtgg aaagggtgag ggagtggcag tgagcacagc
     1801 agggtcgagc aggctatgta gctggaatgg agtgcgagca agctcaagag ttttctcctt
     1861 tggattttcc ggtagatttg taatgctcga gagtagaaat tccaggccaa ctccctcatt
     1921 tgatgtcccg aggggagcaa gggtcttctt ctcatgggaa cggcggtcaa attctcggtg
     1981 gattgaaacg gggcctagct gggaatttct catcgaaaga aattttcagc gctgaaatac
     2041 gtccgagaga tcaagtaagg ttttgataac agtgcagaag gggatattgt ctggtgggtt
     2101 gggattgttg ctggcgcagt caaacgaggg aaatactcct aagcgtggtt ctgctgtctt
     2161 tgtcatattt caaagtcttc atctgttttt catcacccag cgagtgtgag aagtctgaat
     2221 gtgtgagcag aaataaaagg gaatttcgag ccataagaac cagctaaaga acatcaaacg
     2281 agctgtgagc ggactcgtct cttagactta agattcactc aaacgtcatt gcgtgtaact
     2341 tgttcctagg actgcttttt tggtttgctg ccgtcgatat cgtcacacgt tttgtagtct
     2401 tccattctcg tgattacccc ggaattattt tcgtcttcac ctgaacgatt ttcttacaaa
     2461 gtggacgaga tgaacgtgct tgttctctcg agggccccgg atcgaccttc tgctttttcg
     2521 tcctcgtttg atctcctggg atccgagagc ttggtcactc gtcgatgttt tcctcgttag
     2581 aaaaagtgta atgggttagc agtcccctgc atcagactgt caatttccat ttgttttaat
     2641 tggaagaagg cccggagttt cccgggttcg tctttactct gttcgtatag tgtcgcgtgt
     2701 gcccagttgt tgtatttcca gagtgtacct tgcggtggag ttgacttttt cgtgccacat
     2761 gaatgtaaac cgtttgagca ctattatctg ttaaggctgc taatcaacac tgacctctca
     2821 attgcgctgt atgtggatgc agttaggagc cttgtcgaat aatgtgagac cctgcagtcc
     2881 ggcaaaaaag ggcaaggtgt caccaccctg ccctttttct ttaaaaccga aaagattact
     2941 tcgcgttatg caggcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     3001 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     3061 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     3121 gctggcgttt ttccacaggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     3181 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     3241 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     3301 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     3361 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     3421 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     3481 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     3541 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     3601 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     3661 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     3721 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     3781 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     3841 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagct cgaggcttgg
     3901 attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
     3961 ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg
     4021 ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga
     4081 agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
     4141 gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt
     4201 ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa
     4261 taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata
     4321 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag
     4381 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
     4441 ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa
     4501 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
     4561 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
     4621 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
     4681 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
     4741 cattatggtg aaagttggaa cctcttacgt gcccgatcaa ctcgagtg
//
