LOCUS       sequence-275042-        5903 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5903)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 5903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-Myotilin-C-14
FEATURES             Location/Qualifiers
     source          1..5903
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        142..345
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     296..316
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             390..1067
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     791..810
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     985..1008
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    2733..2854
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2770..2789)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2824..2843
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2861..3316)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2998..3019)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3210..3229
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3343..3447
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3472..3492)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3516..3726
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        3538..3734
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3585..3720
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3769..4563
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3769..4563
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3823..3842)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4433..4452
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4751..4770)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4795..4842
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5171..5759
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5660..5679
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
       61 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      121 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
      181 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
      241 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
      301 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
      361 cagatccgct agcgctaccg gtggctacca tggtgagcaa gggcgaggag gtcatcaagg
      421 agttcatgcg cttcaaggag cacatggagg gctccgtgaa cggccacgag ttcgagatcg
      481 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaggctg aaggtgacca
      541 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagatcatg tacggctcca
      601 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
      661 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
      721 actcctccct gcaggacggc gagttcatct acaaggtgaa ggtgcgcggc accaacttcc
      781 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
      841 tgtaccccga ggacggcgcc ctgaagggcg agatgaagat gaggctgagg ctgaaggacg
      901 gcggccacta cgacgccgag gtcaagacca cctacatggc caagaagccc gtgcagctgc
      961 ccggcgccta caagaccgac atcaagctgg acatcacctc ccacaacgag gactacacca
     1021 tcgtggaaca gtacgagcgc gccgagggcc gccactccac cggcgcctcc ggaagtgctg
     1081 ggagcgcaag cggaagcgga ggctccggtt ttaactacga acgtccaaaa cacttcatcc
     1141 agtcccaaaa cccatgtggc tccagattgc agcctcctgg accagaaacc tccagcttct
     1201 ctagccagac caaacagtct tccattatca tccagccccg ccagtgtaca gagcaaagat
     1261 tttctgcctc ctcaacactg agctctcaca tcaccatgtc ctcctctgct ttccctgctt
     1321 ctccccagca gcatgctggc tccaacccag gccaaagggt tacaaccacc tataaccagt
     1381 ccccagccag cttcctcagc tccatattac catcacagcc tgattacaat agcagtaaaa
     1441 tcccttccgc tatggattcc aactatcaac agtcctcagc tggccaacct ataaatgcaa
     1501 agccatccca aactgcaaat gctaagccca taccaagaac tcctgatcat gaaatacaag
     1561 gatcaaaaga agctttgatt caagatttgg aaagaaagct gaaatgcaag gacacccttc
     1621 ttcataatgg aaatcaacgt ctaacatatg aagagaagat ggctcgcaga ttgctaggac
     1681 cacagaatgc agctgctgtg tttcaagctc aggatgacag tggtgcacaa gactcgcagc
     1741 aacacaactc agaacatgcg cgactgcaag ttcctacatc acaagtaaga agtagatcaa
     1801 cctcaagggg agatgtgaat gatcaggatg caatccagga gaaattttac ccaccacgtt
     1861 tcattcaagt gccagagaac atgtcgattg atgaaggaag attctgcaga atggacttca
     1921 aagtgagtgg actgccagct cctgatgtgt catggtatct aaatggaaga acagttcaat
     1981 cagatgattt gcacaaaatg atagtgtctg agaagggtct tcattcactc atctttgaag
     2041 tagtcagagc ttcagatgca ggggcttatg catgtgttgc caagaataga gcaggagaag
     2101 ccaccttcac tgtgcagctg gatgtccttg caaaagaaca taaaagagca ccaatgttta
     2161 tctacaaacc acagagcaaa aaagttttag agggagattc agtgaaacta gaatgccaga
     2221 tctcggctat acctccacca aagcttttct ggaaaagaaa taatgaaatg gtacaattca
     2281 acactgaccg aataagctta tatcaagata acactggaag agttacttta ctgataaaag
     2341 atgtaaacaa gaaagatgct gggtggtata ctgtgtcagc agttaatgaa gctggagtga
     2401 ctacatgtaa cacaagatta gacgttacgg cacgtccaaa ccaaactctt ccagctccta
     2461 agcagttacg ggttcgacca acattcagca aatatttagc acttaatggg aaaggtttga
     2521 atgtaaaaca agcttttaac ccagaaggag aatttcagcg tttggcagct caatctggac
     2581 tctatgaaag tgaagaactt taaggatcca ccggatctag ataactgatc ataatcagcc
     2641 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     2701 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     2761 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     2821 gttgtggttt gtccaaactc atcaatgtat cttaaggcgt aaattgtaag cgttaatatt
     2881 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     2941 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     3001 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     3061 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     3121 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     3181 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     3241 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     3301 ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     3361 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     3421 cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt
     3481 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     3541 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     3601 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     3661 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     3721 taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     3781 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     3841 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     3901 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca
     3961 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     4021 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     4081 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     4141 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     4201 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     4261 ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc
     4321 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     4381 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     4441 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     4501 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     4561 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
     4621 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     4681 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg
     4741 ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa
     4801 taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt
     4861 cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg
     4921 tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca
     4981 acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt tagattgatt
     5041 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     5101 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     5161 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     5221 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     5281 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     5341 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     5401 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     5461 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     5521 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     5581 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     5641 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     5701 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     5761 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     5821 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg cattagttat
     5881 taatagtaat caattacggg gtc
//
