LOCUS       sequence-275044-        5933 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5933)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 5933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Myotilin-C-14
FEATURES             Location/Qualifiers
     source          1..5933
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        122..325
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     276..296
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      364..373
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             370..1077
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(515..533)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     783..802
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    2743..2864
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2780..2799)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2834..2853
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2871..3326)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3008..3029)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3220..3239
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3353..3457
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3482..3502)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3526..3736
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        3548..3744
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3595..3730
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3779..4573
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3779..4573
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3833..3852)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4443..4462
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4761..4780)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4805..4852
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5181..5769
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5670..5689
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tggagttccg cgttacataa cttacggtaa atggcccgcc tggcattatg cccagtacat
       61 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      121 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      181 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      241 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
      301 gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct agcgctaccg
      361 gtcgccacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
      421 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
      481 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
      541 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
      601 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
      661 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
      721 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
      781 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
      841 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
      901 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
      961 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
     1021 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtcc
     1081 ggaagtgctg ggagcgcaag cggaagcgga ggctccggtt ttaactacga acgtccaaaa
     1141 cacttcatcc agtcccaaaa cccatgtggc tccagattgc agcctcctgg accagaaacc
     1201 tccagcttct ctagccagac caaacagtct tccattatca tccagccccg ccagtgtaca
     1261 gagcaaagat tttctgcctc ctcaacactg agctctcaca tcaccatgtc ctcctctgct
     1321 ttccctgctt ctccccagca gcatgctggc tccaacccag gccaaagggt tacaaccacc
     1381 tataaccagt ccccagccag cttcctcagc tccatattac catcacagcc tgattacaat
     1441 agcagtaaaa tcccttccgc tatggattcc aactatcaac agtcctcagc tggccaacct
     1501 ataaatgcaa agccatccca aactgcaaat gctaagccca taccaagaac tcctgatcat
     1561 gaaatacaag gatcaaaaga agctttgatt caagatttgg aaagaaagct gaaatgcaag
     1621 gacacccttc ttcataatgg aaatcaacgt ctaacatatg aagagaagat ggctcgcaga
     1681 ttgctaggac cacagaatgc agctgctgtg tttcaagctc aggatgacag tggtgcacaa
     1741 gactcgcagc aacacaactc agaacatgcg cgactgcaag ttcctacatc acaagtaaga
     1801 agtagatcaa cctcaagggg agatgtgaat gatcaggatg caatccagga gaaattttac
     1861 ccaccacgtt tcattcaagt gccagagaac atgtcgattg atgaaggaag attctgcaga
     1921 atggacttca aagtgagtgg actgccagct cctgatgtgt catggtatct aaatggaaga
     1981 acagttcaat cagatgattt gcacaaaatg atagtgtctg agaagggtct tcattcactc
     2041 atctttgaag tagtcagagc ttcagatgca ggggcttatg catgtgttgc caagaataga
     2101 gcaggagaag ccaccttcac tgtgcagctg gatgtccttg caaaagaaca taaaagagca
     2161 ccaatgttta tctacaaacc acagagcaaa aaagttttag agggagattc agtgaaacta
     2221 gaatgccaga tctcggctat acctccacca aagcttttct ggaaaagaaa taatgaaatg
     2281 gtacaattca acactgaccg aataagctta tatcaagata acactggaag agttacttta
     2341 ctgataaaag atgtaaacaa gaaagatgct gggtggtata ctgtgtcagc agttaatgaa
     2401 gctggagtga ctacatgtaa cacaagatta gacgttacgg cacgtccaaa ccaaactctt
     2461 ccagctccta agcagttacg ggttcgacca acattcagca aatatttagc acttaatggg
     2521 aaaggtttga atgtaaaaca agcttttaac ccagaaggag aatttcagcg tttggcagct
     2581 caatctggac tctatgaaag tgaagaactt taaggatcca ccggatctag ataactgatc
     2641 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     2701 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     2761 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     2821 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaaggcgt aaattgtaag
     2881 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     2941 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     3001 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     3061 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     3121 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     3181 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     3241 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     3301 gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac
     3361 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     3421 ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg
     3481 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     3541 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     3601 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     3661 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     3721 tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat
     3781 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     3841 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     3901 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     3961 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     4021 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     4081 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     4141 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     4201 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     4261 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg
     4321 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     4381 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     4441 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     4501 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     4561 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     4621 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     4681 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     4741 caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct
     4801 atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc
     4861 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa
     4921 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg
     4981 ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt
     5041 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     5101 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     5161 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     5221 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     5281 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     5341 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     5401 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     5461 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     5521 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     5581 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     5641 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     5701 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     5761 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     5821 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg
     5881 cattagttat taatagtaat caattacggg gtcattagtt catagcccat ata
//
