LOCUS       sequence-275049-        8465 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express HIV Env which is isolated a from IRB approved HIV infected 
            patient samples from Singapore and cloned into expression vector 
            (pcDNA 3.1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8465)
  AUTHORS   Joshi A, Cox EK, Sedano MJ, Punke EB, Lee RT, Maurer-Stroh S, Kaur 
            P, Ng OT, Garg H
  TITLE     HIV-1 subtype CRF01_AE and B differ in utilization of low levels of 
            CCR5, Maraviroc susceptibility and potential N-glycosylation sites.
  JOURNAL   Virology. 2017 Dec;512:222-233. doi: 10.1016/j.virol.2017.09.026. 
            Epub 2017 Oct 9.
  PUBMED    29020646
REFERENCE   2  (bases 1 to 8465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virology.";
            date: "2017-12"; volume: "512:222-233. doi"; pages: " 
            10.1016/j.virol.2017.09.026. Epub 2017 Oct 9"
COMMENT     Sequence Label: pHM045Env
FEATURES             Location/Qualifiers
     source          1..8465
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3963..4004
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4014..4031
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4054..4071)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4060..4284
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4330..4758
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4417..4436)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4627..4648
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4767..4787)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4772..5101
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4952..5087
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5014..5033
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5168..5962
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5222..5241)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5832..5851
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6138..6259
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6175..6194)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6229..6248
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6308..6324)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6308..6324)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6321..6343)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6332..6348
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6356..6362,6363..6380,6381..6386))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6401..6422
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6539..6556)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6710..7298)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6790..6809)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7469..8260,8261..8329))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8092..8111
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8330..8434)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttggt accgagctcg gatccagtac ccttcaccga tcaagcttta ggcatctcct
      961 atggcaggaa gaagcggaag caccgacgag gaactcctcg cagcagtaag agtcatcaag
     1021 atcctatacc agagcagtga gtatatgtaa tgacaccttt agaagttagt acaataatag
     1081 gactgatagt agcatttatc ttagcaataa tagtatggac tatagtagct atagaactta
     1141 agaaaatact aagagagaga agaatagaaa gattaattaa gagaataaga gagagagcag
     1201 aagacagtgg aaatgagagt gagggggaca cggatgaatt ggcaacactt gtggaaatgg
     1261 gggactttga tccttgggtt ggggataatt tgtagtgcct cagaaaactt gtgggttaca
     1321 gtttattatg gggttcctgt gtggagagat gcagacacca ccctattttg tgcatcagat
     1381 gcccaagcac ataagacaga agtgcacaat atctgggcca cacatgcctg tgtacccaca
     1441 gaccccaacc cacaagaaat actcctggaa aatgtaacag aaaattttaa catgtggaaa
     1501 aataacatgg tggagcagat gcaggaagat gtaatcagtt tatgggatca aagtctaaag
     1561 ccatgtgtaa agttaactcc tctctgtgtt actttaaatt gtaccaatgc tcatctgcat
     1621 gcaaatttca ctagcacaaa tctcactagc acaataggaa atataacaga ggaagtaaga
     1681 aactgctctt ttaatatgac cacagaacta aaagataaga agcagaaggt ccatgcactt
     1741 ttttataagc ttgatatagt accaattgat aataatagta atagtagtga gtataggcta
     1801 ataaactgta atacttcagt cattaagcag gcttgtccaa aggtatcctt tgatccaatt
     1861 cctatacatt attgtactcc agctggttat gcgattttaa aatgtaatga taagaatttc
     1921 aatgggacag ggccatgtaa aaatgttagc tcagtacaat gcacacatgg aattaagcca
     1981 gtggtatcaa ctcagttgct gttaaatggt agtctagcag aagatgagat agtaatcaga
     2041 agtgaaaatc tcacagacaa tgccaagacc ataatagtgc atcttaataa agctgtaaac
     2101 atcagttgta ccagaccctc caacaataca agaacaggtg tgcatatagg accaggacaa
     2161 gtattctata gaacaggaga catagtagga gatataacaa aagcatattg tgggattaat
     2221 ggtacagaat ggaatgaaac tttaacactg gtaactgaaa aattaaagaa acactttaag
     2281 aataagacaa taatctttca accacactca ggaggagatc tagaaattac aatgcatcat
     2341 tttaattgta gaggggaatt tttctattgc aatacatcaa aactgtttga tggtacttac
     2401 ccaaaaaatg gaacatgtaa tgacactatc atacttccat gtaaaataaa gagaattgta
     2461 agattgtggc aggaagtagg tcaagcaatg tatgctcctc ccatcagtgg aaaaattagg
     2521 tgtgactcaa atattacagg aatactattg acaagagatg gtggtaatgg taatacaagt
     2581 aataatgaaa ccttcagacc tggaggagga aatataaagg acaattggag aagtgaatta
     2641 tataaatata aagtagtaca aattgaacca ctaggaatag cacccaccag ggcaaaaaga
     2701 agagtggtgg agagagaaaa aagagcagtg ggaataggag ctatgatctt tgggttctta
     2761 ggagcagcag gaagcactat gggcgcagcg tcaataacgc tgacggtaca ggccagacaa
     2821 ttattgtctg gtatagtgca acagcaaagc aatttgctga gggctataga ggcgcagcag
     2881 catctgttgc aactcacagt ctggggcatt aaacagctcc aggcaagagt cctggctgtg
     2941 gaaagctacc taaaagatca aaagttccta ggactttggg gctgctctgg gaaaaccatc
     3001 tgcaccactg ctgtgccctg gaacaccact tggagtaata aatcttatga tgagatttgg
     3061 aacaacatga catggataga atgggagaga gaaattagca attacacaag ccaaatatat
     3121 ggaatactta cagaatcgca gaaccagcag gaaaagaatg aaaaggatct gttagaattg
     3181 gataaatggg caagtctgtg gaattggttt agcataacaa gttggctgtg gtatataaaa
     3241 atatttataa taatagtagg aagtttaata ggtttaagaa taatttttgc tgtgctttct
     3301 atagtaaata gagttaggca gggatactca cccttgtctt tccagacccc tccccatcac
     3361 cagagggaac ccgacaggcc agaagaaata gaagaaggag gtggcgagca aggcagaggc
     3421 agatccgtgc gattagtgac cggattctta ccacttatct gggacgatct ccggagcctg
     3481 tgcctcttca gctaccaccg cttgagagac ttcatcttga ttgtaacgag gactgtggaa
     3541 cttctgggac gcagcagtct caagggactg agacgggggt gggaaggcct caaatatctg
     3601 gggaatcttc tgatatattg ggggcaggaa ctaaaaacta gtgctatttc tttgtttgat
     3661 gcagcagcag tagcagtagc agggtggaca gatagggtta tagaagtagc acaaagagct
     3721 tggagagcct ttctccacat acctagaaga atcagacagg gcttagaaag ggctttgcta
     3781 taacatggga aacaagtggt caaaaacatg gcctcgggtc agggaaagat tagagaaaac
     3841 tcctccagca gcagatggag taggagcagt atctcaagac ggatccagct aagggtcaag
     3901 acaattctgc agatatccag cacagtggcg gccgctcgag tctagagggc ccgcggttcg
     3961 aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc
     4021 accatcacca ttgagtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     4081 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     4141 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     4201 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     4261 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     4321 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     4381 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     4441 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     4501 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     4561 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     4621 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     4681 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     4741 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     4801 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     4861 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     4921 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     4981 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     5041 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     5101 aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc
     5161 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     5221 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     5281 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     5341 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     5401 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     5461 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     5521 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     5581 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     5641 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     5701 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     5761 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     5821 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     5881 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     5941 ccttcttgac gagttcttct gagcgggact ctggggttcg cgaaatgacc gaccaagcga
     6001 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     6061 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     6121 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     6181 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     6241 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     6301 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     6361 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     6421 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     6481 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     6541 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     6601 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     6661 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6721 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6781 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6841 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6901 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6961 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     7021 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     7081 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     7141 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     7201 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     7261 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     7321 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     7381 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     7441 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     7501 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     7561 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     7621 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     7681 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     7741 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     7801 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     7861 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     7921 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     7981 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     8041 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     8101 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     8161 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     8221 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     8281 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     8341 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     8401 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     8461 acgtc
//
