LOCUS       sequence-275053-       11040 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter to evaluate YAP1/TEAD-mediated gene transcription.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11040)
  AUTHORS   Maruyama J, Inami K, Michishita F, Jiang X, Iwasa H, Nakagawa K, 
            Ishigami-Yuasa M, Kagechika H, Miyamura N, Hirayama J, Nishina H, 
            Nogawa D, Yamamoto K, Hata Y
  TITLE     Novel YAP1 Activator, Identified by Transcription-Based Functional 
            Screen, Limits Multiple Myeloma Growth.
  JOURNAL   Mol Cancer Res. 2018 Feb;16(2):197-211. doi: 
            10.1158/1541-7786.MCR-17-0382. Epub 2017 Oct 23.
  PUBMED    29061667
REFERENCE   2  (bases 1 to 11040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/1541-7786.MCR-17-0382"; journalName: "Mol Cancer Res"; 
            date: "2018-02"; volume: "16"; issue: "2"; pages: "197-211"
COMMENT     Sequence Label: pLL3.7 FLAG-YAP1-TEAD-P-H2B-mCherry
FEATURES             Location/Qualifiers
     source          1..11040
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    337..428
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(369..388)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    442..490
                     /note="synthetic polyadenylation signal"
     enhancer        586..889
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        890..1093
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             1132..1155
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1162..1179
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    join(2725..3299,3300..3302,3303..3311)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2892..2909)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3119..3138
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3312..3314,3315..3317,3318..4031)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3312..3314,3315..3317,3318..4028)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3357..3378)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3618..3637)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3965..3986
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(4057..4090)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    4146..4734
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4199..4219)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4617..4628)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4737..4753)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             5263..5443
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(5505..6093)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5585..5604)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6264..7055,7056..7124))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6887..6906
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7125..7229)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7304..7323)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7495..7874
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7876..8074
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             8092..8272
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8319..8444
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8941..9174
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9359..9403
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9701..9818
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(9877..10587)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(10155..10174)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     10424..10442
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      10584..10593
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(10606..10983)
                     /codon_start=1
                     /gene="HIST1H2BB"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPSKSAPAPKKGSKKAITKAQKKDGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSSK"
ORIGIN
        1 gccctggctg gtcgctccgt gctctccggt ctcacagccc cgcttttacg tgccccggag
       61 cctgggctgg ccctgcaggt cgacggtatc gataagcttg atggacacac attccacagc
      121 tggggacaca cattccacag ctggggacac acattccaca gctggggaca cacattccac
      181 agctggggac acacattcca cagctgggga cacacattcc acagctgggg acacacattc
      241 cacagctggg gacacacatt ccacagcatc gaattcctgc agcccggggg atctcgagcc
      301 cgggctagca cgcgtaagag ctcggtacct atcgatagag aaatgttctg gcacctgcac
      361 ttgcactggg gacagcctat tttgctagtt tgttttgttt cgttttgttt tgatggagag
      421 cgtatgttag tactatcgat tcacacaaaa aaccaacaca cagatgtaat gaaaataaag
      481 atattttatt gcggccgcgt ggataaccgt attaccgcca tgcattagtt attaatagta
      541 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
      601 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
      661 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
      721 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
      781 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      841 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
      901 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
      961 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     1021 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     1081 tataagcaga gctggtttag tgaaccgtca gatccgctag ccggggccat ggactacaaa
     1141 gacgatgacg acaaggaatt gcaccatcat catcatcatg aattcacgcg tcccgggcag
     1201 cagccgccgc ctcaaccggc cccccagggc caagggcagc cgccttcgca gcccccgcag
     1261 gggcagggcc cgccgtccgg acccgggcaa ccggcacccg cggcgaccca ggcggcgccg
     1321 caggcacccc ccgccgggca tcagatcgtg cacgtccgcg gggactcgga gaccgacctg
     1381 gaggcgctct tcaacgccgt catgaacccc aagacggcca acgtgcccca gaccgtgccc
     1441 atgaggctcc ggaagctgcc cgactccttc ttcaagccgc cggagcccaa atcccactcc
     1501 cgacaggcca gtactgatgc aggcactgca ggagccctga ctccacagca tgttcgagct
     1561 cattcctctc cagcttctct gcagttggga gctgtttctc ctgggacact gacccccact
     1621 ggagtagtct ctggcccagc agctacaccc acagctcagc atcttcgaca gtcttctttt
     1681 gagatacctg atgatgtacc tctgccagca ggttgggaga tggcaaagac atcttctggt
     1741 cagagatact tcttaaatca catcgatcag acaacaacat ggcaggaccc caggaaggcc
     1801 atgctgtccc agatgaacgt cacagccccc accagtccac cagtgcagca gaatatgatg
     1861 aactcggctt caggtcctct tcctgatgga tgggaacaag ccatgactca ggatggagaa
     1921 atttactata taaaccataa gaacaagacc acctcttggc tagacccaag gcttgaccct
     1981 cgttttgcca tgaaccagag aatcagtcag agtgctccag tgaaacagcc accacccctg
     2041 gctccccaga gcccacaggg aggcgtcatg ggtggcagca actccaacca gcagcaacag
     2101 atgcgactgc agcaactgca gatggagaag gagaggctgc ggctgaaaca gcaagaactg
     2161 cttcggcagg caatgcggaa tatcaatccc agcacagcaa attctccaaa atgtcaggag
     2221 ttagccctgc gtagccagtt accaacactg gagcaggatg gtgggactca aaatccagtg
     2281 tcttctcccg ggatgtctca ggaattgaga acaatgacga ccaatagctc agatcctttc
     2341 cttaacagtg gcacctatca ctctcgagat gagagtacag acagtggact aagcatgagc
     2401 agctacagtg tccctcgaac cccagatgac ttcctgaaca gtgtggatga gatggataca
     2461 ggtgatacta tcaaccaaag caccctgccc tcacagcaga accgtttccc agactacctt
     2521 gaagccattc ctgggacaaa tgtggacctt ggaacactgg aaggagatgg aatgaacata
     2581 gaaggagagg agctgatgcc aagtctgcag gaagctttga gttctgacat ccttaatgac
     2641 atggagtctg ttttggctgc caccaagcta gataaagaaa gctttcttac atggttatag
     2701 gtcgacccgg gcggccggga tccgcccctc tccctccccc ccccctaacg ttactggccg
     2761 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
     2821 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
     2881 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
     2941 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
     3001 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
     3061 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     3121 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
     3181 gggatctgat ctggggcctc ggtacacatg ctttacatgt gtttagtcga ggttaaaaaa
     3241 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata
     3301 tggccacaac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     3361 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     3421 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     3481 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     3541 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     3601 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     3661 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     3721 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     3781 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     3841 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     3901 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     3961 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     4021 tgtacaagta aagcggccaa ttcgtcgagg gacctaataa cttcgtatag catacattat
     4081 acgaagttat acatgtttaa gggttccggt tccactaggt acaattcgat atcaagctta
     4141 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     4201 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     4261 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     4321 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     4381 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     4441 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     4501 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     4561 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     4621 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     4681 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     4741 accgtcgacc tcgatcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca
     4801 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca
     4861 gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     4921 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc
     4981 cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa ctacacacca
     5041 gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
     5101 caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca ccctgtgagc
     5161 ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga cagccgccta
     5221 gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct ctggttagac
     5281 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     5341 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     5401 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagcatgtg agcaaaaggc
     5461 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     5521 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     5581 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     5641 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     5701 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     5761 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     5821 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     5881 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     5941 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     6001 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     6061 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     6121 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     6181 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     6241 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     6301 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     6361 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     6421 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     6481 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     6541 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     6601 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     6661 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     6721 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     6781 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     6841 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     6901 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     6961 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     7021 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     7081 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     7141 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     7201 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     7261 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
     7321 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
     7381 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
     7441 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
     7501 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     7561 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     7621 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     7681 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     7741 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     7801 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     7861 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     7921 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     7981 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     8041 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
     8101 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     8161 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     8221 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     8281 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     8341 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     8401 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     8461 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     8521 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     8581 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     8641 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     8701 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     8761 gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat
     8821 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     8881 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     8941 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     9001 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     9061 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     9121 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     9181 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     9241 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     9301 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     9361 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     9421 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     9481 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     9541 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     9601 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat cggcactgcg
     9661 tgcgccaatt ctgcagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     9721 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     9781 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     9841 gagatccagt ttggttagta ccgggcccgc tctagattac ttgtacagct cgtccatgcc
     9901 gccggtggag tggcggccct cggcgcgttc gtactgttcc acgatggtgt agtcctcgtt
     9961 gtgggaggtg atgtccaact tgatgttgac gttgtaggcg ccgggcagct gcacgggctt
    10021 cttggccttg taggtggtct tgacctcagc gtcgtagtgg ccgccgtcct tcagcttcag
    10081 cctctgcttg atctcgccct tcagggcgcc gtcctcgggg tacatccgct cggaggaggc
    10141 ctcccagccc atggtcttct tctgcattac ggggccgtcg gaggggaagt tggtgccgcg
    10201 cagcttcacc ttgtagatga actcgccgtc ctgcagggag gagtcctggg tcacggtcac
    10261 cacgccgccg tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg ggaaggacag
    10321 cttcaagtag tcggggatgt cggcggggtg cttcacgtag gccttggagc cgtacatgaa
    10381 ctgaggggac aggatgtccc aggcgaaggg cagggggcca cccttggtca ccttcagctt
    10441 ggcggtctgg gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct cgaactcgtg
    10501 gccgttcacg gagccctcca tgtgcacctt gaagcgcatg aactccttga tgatggccat
    10561 gttatcctcc tcgcccttgc tcaccatggt ggcgaccggt ggatccttag agctagtgta
    10621 cttggtaact gccttagtgc cctcggacac agcatgctta gccagctccc caggcagcag
    10681 caggcgcaca gccgtctgaa tctccctgga ggtgatggtc gagcgcttat tgtagtgagc
    10741 caggcgagaa gcctcgcccg cgatgcgctc gaagatgtcg ttgacgaagg agttcatgat
    10801 ccccatggcc ttggatgaga tgccggtgtc ggggtggacc tgcttcagaa ccttgtacac
    10861 atagatagaa tagctctcct tgcggctgcg cttacgcttc ttaccatcct tcttctgcgc
    10921 cttagtgata gccttcttag aacccttttt aggggctgga gcagacttag agggttcagg
    10981 catggtggcg gtaccaagct tggctgcccg cacagccctg ctcaccgtct ccgcagctca
//
