LOCUS       sequence-275054-         501 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GST-SUMO tagged CTA2 Subunit with 11th Strand.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Luong P, Li Q, Chen PF, Wrighton PJ, Chang D, Dwyer S, Bayer MT, 
            Snapper SB, Hansen SH, Thiagarajah JT, Goessling W, Lencer WI
  TITLE     A quantitative single-cell assay for retrograde membrane traffic 
            enables rapid detection of defects in cellular organization.
  JOURNAL   Mol Biol Cell. 2019 Nov 27:mbcE19070375. doi: 
            10.1091/mbc.E19-07-0375.
  PUBMED    31774722
REFERENCE   2  (bases 1 to 501)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2019 Nov 27:mbcE19070375. doi: 10.1091/mbc.E19-07-0375."
COMMENT     Sequence Label: CTA2-mNG211
FEATURES             Location/Qualifiers
     source          1..501
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..294
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
ORIGIN
        1 atgtcggact cagaagtcaa tcaagaagct aagccagagg tcaagccaga agtcaagcct
       61 gagactcaca tcaatttaaa ggtgtccgat ggatcttcag agatcttctt caagatcaaa
      121 aagaccactc ctttaagaag gctgatggaa gcgttcgcta aaagacaggg taaggaaatg
      181 gactccttaa gattcttgta cgacggtatt agaattcaag ctgatcagac ccctgaagat
      241 ttggacatgg aggataacga tattattgag gctcacagag aacagattgg tggtaccgag
      301 ctcaacttca aggagtggca aaaggccttt accgatatga tgggaggtgg aggttcctcg
      361 atgagtaata cttgcgatga aaaaacccaa agtctaggtg taaaattcct tgacgaatac
      421 caatctaaag ttaaaagaca aatattttca ggctatcaat ctgatattga tacacataat
      481 agaattaagg atgaattatg a
//
