LOCUS       sequence-275056-         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Membrane Tethered Split Fluorescent Reporter for ER Retragrade.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Luong P, Li Q, Chen PF, Wrighton PJ, Chang D, Dwyer S, Bayer MT, 
            Snapper SB, Hansen SH, Thiagarajah JT, Goessling W, Lencer WI
  TITLE     A quantitative single-cell assay for retrograde membrane traffic 
            enables rapid detection of defects in cellular organization.
  JOURNAL   Mol Biol Cell. 2019 Nov 27:mbcE19070375. doi: 
            10.1091/mbc.E19-07-0375.
  PUBMED    31774722
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2019 Nov 27:mbcE19070375. doi: 10.1091/mbc.E19-07-0375."
COMMENT     Sequence Label: Sec61b-HA-mNG21-10
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..288
                     /codon_start=1
                     /gene="SEC61B"
                     /product="human Sec61-beta subunit of the translocon in the
                     endoplasmic reticulum"
                     /label=Sec61-beta
                     /translation="MPGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAG
                     RTTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS"
     CDS             298..324
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      580..589
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atgcctggtc cgacccccag tggcactaac gtgggatcct cagggcgctc tcccagcaaa
       61 gcagtggccg cccgggcggc gggatccact gtccggcaga ggaaaaatgc cagctgtggg
      121 acaaggagtg caggccgcac aacctcggca ggcaccgggg ggatgtggcg attctacaca
      181 gaagattcac ctgggctcaa agttggccct gttccagtat tggttatgag tcttctgttc
      241 atcgcttctg tatttatgtt gcacatttgg ggcaagtaca ctcgttcggg aggaggatac
      301 ccatacgatg ttccagatta cgctatggtg agcaagggtg aggaggataa catggcctct
      361 ctcccagcga ctcatgagtt acacatcttt ggctccatca acggtgtgga ctttgacatg
      421 gtgggtcagg gtaccggcaa tccaaatgat ggttatgagg agttaaacct gaagtccacc
      481 aagggtgacc tccagttctc cccctggatt ctggtccctc atatcgggta tggcttccat
      541 cagtacctgc cctaccctga cgggatgtcg cctttccagg ccgccatggt agatggctcc
      601 ggataccaag tccatcgcac aatgcagttt gaagatggtg cctcccttac tgttaactac
      661 cgctacacct acgagggaag ccacatcaaa ggagaggccc aggtgatggg gactggtttc
      721 cctgctgacg gtcctgtgat gaccaacacg ctgaccgctg cggactggtg catgtcgaag
      781 aagacttacc ccaacgacaa aaccatcatc agtaccttta agtggagtta caccactgta
      841 aatggcaaac gctaccggag cactgcgcgg accacctaca cctttgccaa gccaatggcg
      901 gctaactatc tgaagaacca gccgatgtac gtgttccgta agacggagct caagcactcc
      961 atg
//
