LOCUS       sequence-275080-       11418 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nup62 dimerization construct fused to 2 copies of GFP under an CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11418)
  AUTHORS   Dharan A, Bachmann N, Talley S, Zwikelmaier V, Campbell EM
  TITLE     Nuclear pore blockade reveals that HIV-1 completes reverse 
            transcription and uncoating in the nucleus.
  JOURNAL   Nat Microbiol. 2020 Jun 1. pii: 10.1038/s41564-020-0735-8. doi: 
            10.1038/s41564-020-0735-8.
  PUBMED    32483230
REFERENCE   2  (bases 1 to 11418)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Microbiol. 2020 Jun 1. pii: 10.1038/s41564-020-0735-8. doi: 
            10.1038/s41564-020-0735-8."
COMMENT     Sequence Label: pLVX Nup62Fv2GFP
FEATURES             Location/Qualifiers
     source          1..11418
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1302..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2027..2142
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2200..2503
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2504..2707
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2658..2678
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2704..2728
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4389..4709
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     regulatory      4716..4725
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4722..4724,4725..4727,4728..5435)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     CDS             join(4722..4724,4725..4727,4728..5435)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     CDS             join(5439..5441,5442..5444,5445..6158)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5439..5441,5442..5444,5445..6155)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     promoter        6252..6751
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6287..6310)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6684..6703
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6772..7371
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6772..6791)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7268..7288
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7385..7973
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7438..7458)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7856..7867)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8180..8813
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8942..8958)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8942..8958)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8955..8977)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8966..8982
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8990..8996,8997..9014,9015..9020))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9035..9056
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9173..9190)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9344..9932)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9424..9443)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10103..10894,10895..10963))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10726..10745
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10964..11068)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    11116..11250
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11153..11172)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11207..11226
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(11418,1..633)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
       61 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
      121 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
      181 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
      241 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
      301 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
      361 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
      421 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg
     2041 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2101 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2161 cagcagagat ccagtttatc gataagcttg ggagttccgc gttacataac ttacggtaaa
     2221 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     2281 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     2341 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     2401 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     2461 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     2521 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     2581 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     2641 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     2701 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     2761 ccatagaaga caccgactct actagaggat caccggactc agatctcgag gccaccatga
     2821 gcgggtttaa ttttggaggc actggggccc ctacaggcgg gttcacgttt ggcactgcaa
     2881 agacggcaac aaccacacct gctacagggt tttctttctc cacctctggc actggagggt
     2941 ttaattttgg ggctcccttc caaccagcca caagtacccc ttccaccggc ctgttctcac
     3001 ttgccaccca gactccggcc acacagacga caggcttcac ttttggaaca gcgactcttg
     3061 cttcgggggg aactggattt tctttgggga tcggtgcttc aaagctcaac ttgagcaaca
     3121 cagctgccac cccagccatg gcaaacccca gcggctttgg gctgggcagc agcaacctca
     3181 ctaatgccat atcgagcacc gtcacctcca gccagggcac agcacccacc ggctttgtgt
     3241 ttggcccctc caccacctct gtggctccag ctaccacatc tggaggcttc tcattcactg
     3301 gtggaagcac ggcccaaccc tccggtttca acattggctc agcagggaat tcagcccagc
     3361 ccacggcacc tgccacgttg cccttcactc cggccacgcc agcagccacc acagcaggtg
     3421 ccacacagcc agctgctccc acacccacag ccaccatcac cagtactggg cccagcctct
     3481 ttgcgtcaat agcaactgct ccaacctcat ctgccaccac tggactctcc ctctgtaccc
     3541 ctgtgaccac agcgggcgcc cccactgctg ggacacaggg cttcagctta aaggcacctg
     3601 gagcagcttc cggcacctcc acaacaacat ccaccgctgc caccgccacc gccaccacca
     3661 ccaccagcag cagcaccacc ggctttgcct tgaatttaaa accactggcg ccagccggga
     3721 tccccagcaa tacagcagct gccgtgaccg ctccacctgg ccctggcgca gctgcagggg
     3781 cggctgccag ctccgccatg acctacgcgc agctggagag cctgatcaac aaatggagcc
     3841 tggagctaga ggaccaggag cggcacttcc tccagcaggc cacccaggtc aacgcctggg
     3901 accgcacgct gatcgagaat ggagaaaaga tcaccagcct gcaccgcgag gtggagaagg
     3961 tgaagctgga ccagaagagg ctggaccagg agctcgactt catcctgtcc cagcagaagg
     4021 agctggaaga cctgctgagc ccactggagg agttggtcaa ggagcagagc gggaccatct
     4081 acctgcagca cgcggatgag gagcgtgaga aaacctacaa gctggctgag aacatcgacg
     4141 cacagctcaa gcgcatggcc caggatctca aggacatcat cgagcacctg aacacgtccg
     4201 gggcccccgc cgacaccagt gacccactgc agcagatctg caagatcctc aatgcgcaca
     4261 tggactcact gcagtggatc gaccagaact cggccctgct gcagaggaag gtggaggagg
     4321 tgaccaaggt gtgcgagggc cggcgcaagg agcaggagcg cagcttccgg atcacctttg
     4381 acacgcgtgg agtgcaggtg gagactatct ccccaggaga cgggcgcacc ttccccaagc
     4441 gcggccagac ctgcgtggtg cactacaccg ggatgcttga agatggaaag aaagttgatt
     4501 cctcccggga cagaaacaag ccctttaagt ttatgctagg caagcaggag gtgatccgag
     4561 gctgggaaga aggggttgcc cagatgagtg tgggtcagag agccaaactg actatatctc
     4621 cagattatgc ctatggtgcc actgggcacc caggcatcat cccaccacat gccactctcg
     4681 tcttcgatgt ggagctccta aaactggaac cggtcgccac catggtgagc aagggcgagg
     4741 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     4801 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     4861 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     4921 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     4981 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     5041 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     5101 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     5161 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     5221 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     5281 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     5341 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     5401 ccgccgggat cactctcggc atggacgagc tgtacggaat ggtgagcaag ggcgaggagc
     5461 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     5521 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     5581 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     5641 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     5701 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     5761 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     5821 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     5881 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     5941 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     6001 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     6061 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     6121 ccgggatcac tctcggcatg gacgagctgt acaagtaaag cggccgcgac tctagatcat
     6181 tcgaattctg cagtcgacgg taccgcgggc ccgggatcca ccggatctag ataactgatc
     6241 ataattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc
     6301 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc
     6361 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc
     6421 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
     6481 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg
     6541 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag
     6601 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc
     6661 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct
     6721 gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac catgaccgag
     6781 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     6841 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     6901 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     6961 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     7021 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     7081 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     7141 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     7201 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     7261 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     7321 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg accgcgtctg
     7381 gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     7441 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     7501 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     7561 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     7621 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     7681 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     7741 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     7801 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     7861 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     7921 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat
     7981 taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct
     8041 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
     8101 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     8161 ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca agatatcctt
     8221 gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg
     8281 ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca
     8341 gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg
     8401 catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca
     8461 tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct
     8521 tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg
     8581 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct
     8641 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     8701 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     8761 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     8821 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     8881 gaggccttga cattgctagc gttttaccgt cgacctctag ctagagcttg gcgtaatcat
     8941 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     9001 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     9061 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     9121 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     9181 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     9241 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     9301 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     9361 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     9421 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     9481 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     9541 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     9601 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     9661 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     9721 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     9781 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     9841 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     9901 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     9961 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
    10021 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
    10081 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
    10141 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    10201 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    10261 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    10321 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    10381 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    10441 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    10501 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    10561 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    10621 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    10681 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    10741 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    10801 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    10861 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    10921 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    10981 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    11041 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
    11101 acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc
    11161 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    11221 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat
    11281 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc
    11341 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
    11401 actacaaact tagtagtt
//
