LOCUS       sequence-275093-       11136 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses fluorescent ALIX S718/721E in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11136)
  TITLE     Plasmids for creating inducible cell lines expressing ALIX-GFP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLVX ALIX-GFP S718/721/E
FEATURES             Location/Qualifiers
     source          1..11136
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2206..2520
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2214..2232
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2250..2268
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2285..2303
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2321..2339
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2357..2375
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2392..2410
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2428..2446
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2493..2517
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(5237..5239,5240..5242,5243..5956)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5237..5239,5240..5242,5243..5953)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5282..5303)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5543..5562)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5890..5911
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        5969..6468
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6004..6027)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6401..6420
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6489..7088
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6489..6508)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6985..7005
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7105..7693
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7158..7178)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7576..7587)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7900..8533
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8661..8677)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8661..8677)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8674..8696)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8685..8701
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8709..8715,8716..8733,8734..8739))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8754..8775
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8892..8909)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9063..9651)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9143..9162)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9822..10613,10614..10682))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10445..10464
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10683..10787)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10835..10969
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10872..10891)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10926..10945
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagtt tactccctat
     2221 cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac gatgtcgagt
     2281 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
     2341 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
     2401 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
     2461 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     2521 tggagaagga tccgccacca tgatggcgac attcatctcg gtgcagctga aaaagacctc
     2581 agaggtggac ctggccaagc cgctggtgaa gttcatccag cagacttacc caagcggcgg
     2641 ggaagagcag gcccagtact gccgcgcggc ggaggagctc agcaagctgc gccgcgccgc
     2701 agtcggtcgt ccgctggaca agcacgaggg cgcgctcgag acgctcctga gatattatga
     2761 tcagatttgt tctattgaac ccaaattccc attttctgaa aatcagatct gcttgacatt
     2821 tacctggaag gatgctttcg ataaaggttc actttttgga ggctctgtaa aactggctct
     2881 tgcaagctta ggatatgaaa agagctgtgt gttgttcaat tgtgcagcct tagctagcca
     2941 aattgcagca gaacagaact tagacaacga tgaaggattg aaaatcgctg ctaaacatta
     3001 ccagtttgct agtggtgcct ttttacatat taaagagacg gttttatctg ccttaagtcg
     3061 agagccgacc gtggacatat ctccagatac tgttgggacc ctcagtctta ttatgctggc
     3121 acaggctcaa gaagtatttt ttttaaaagc cacaagagat aaaatgaaag atgccatcat
     3181 agctaaattg gctaatcagg ctgcagatta ttttggtgat gctttcaaac agtgtcaata
     3241 caaagatact ctccccaagg aggtgttccc tgtcttggct gcaaagcact gtatcatgca
     3301 ggccaatgct gagtaccatc agtctatcct ggcaaaacag cagaagaaat ttggagaaga
     3361 aattgcaagg ttacagcatg cagcagaact gattaaaaca gtggcatctc gctatgatga
     3421 atatgttaat gtgaaggatt tttctgacaa aatcaatcgt gcccttgctg cagcaaagaa
     3481 ggataatgac ttcatttatc atgatcgagt tccagacctt aaagatctag atcctattgg
     3541 caaagccaca cttgtgaaat ctaccccggt caatgtaccc atcagtcaga aatttactga
     3601 tctgtttgag aagatggttc ccgtgtcagt acagcagtct ttggctgcct ataatcagag
     3661 gaaagccgat ttggttaaca gatcaattgc tcagatgaga gaagccacca ctttggcaaa
     3721 tggggtgcta gcttccctta atcttccagc agcaattgaa gatgtgtctg gagacactgt
     3781 acctcagtct atattgacta aatccagatc tgtgattgaa cagggaggca tccagactgt
     3841 tgatcagttg attaaagaac tgcctgaatt actgcaacga aatagagaaa tcctagatga
     3901 gtcattaagg ttgttggatg aagaagaagc aaccgataat gatttaagag caaaatttaa
     3961 ggaacgttgg caaaggacac catccaatga actgtataag cctttaagag cagagggaac
     4021 caacttcaga acagttttag ataaagctgt gcaggcagat ggacaagtga aagaatgtta
     4081 ccagtctcat cgtgacacca tcgtgctttt gtgtaagcca gagcctgagc tgaatgctgc
     4141 catcccttct gctaatccag caaagaccat gcagggcagt gaggttgtaa atgtcttaaa
     4201 atccttattg tcaaatcttg atgaagtaaa gaaggaaaga gagggtctgg agaatgactt
     4261 gaaatctgtg aattttgaca tgacaagcaa gtttttgaca gccctggctc aagatggtgt
     4321 gataaatgaa gaagctcttt ctgttactga actagatcga gtctatggag gtcttacaac
     4381 taaagtccaa gaatctctaa agaaacagga gggacttctt aaaaatattc aggtctcaca
     4441 tcaggaattt tcaaaaatga aacaatctaa taatgaagct aacttaagag aagaagtttt
     4501 gaagaattta gctactgcgt atgacaactt tgttgaactt gtagctaatt tgaaggaagg
     4561 cacaaagttt tacaatgagt tgactgaaat cctggtcagg ttccagaaca aatgcagtga
     4621 tatagttttt gcacggaaga cagaaagaga tgaactctta aaggacttgc aacaaagcat
     4681 tgccagagaa cctgaagctc ctgaaattcc tacacctgcg tatcagtcct caccagcagg
     4741 aggacatgca ccaactcctc caactccagc gccaagaacc atgccgccta ctaagcccca
     4801 gcccccagcc aggcctccac cacctgtgct tccagcaaat cgagctcctt ctgctactgc
     4861 tccatctcca gtgggggctg ggactgctgc gccagctcca tcacaaacgc ctggctcagc
     4921 tcctcctcca caggcgcagg gaccacccta tcccacctat ccaggatatc ctgggtattg
     4981 ccaaatgccc atgcccatgg gctataatcc ttatgcgtat ggccagtata atatgccata
     5041 tccaccagtg tatcaccaga gtcctggaca ggctccatac ccgggacccc agcagccttc
     5101 ataccccttc cctcagcccc cacagcagtc ttactatcca cagcagcccg gggacgagga
     5161 gctcgagcgc cggcttcgcg aggaattgga acggcgcctt cgcgaggagc tggaacgtcg
     5221 actccgcggc tcttccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     5281 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     5341 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     5401 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     5461 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     5521 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     5581 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     5641 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     5701 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     5761 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     5821 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     5881 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     5941 cgagctgtac aagtaataga attctaccgg gtaggggagg cgcttttccc aaggcagtct
     6001 ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct
     6061 cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc
     6121 gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg
     6181 tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc
     6241 gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc
     6301 gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc
     6361 aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag
     6421 cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca
     6481 agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     6541 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     6601 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     6661 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     6721 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     6781 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     6841 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     6901 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     6961 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     7021 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     7081 gtgcctgacg ggcgcgtctg gaacaatcaa cctctggatt acaaaatttg tgaaagattg
     7141 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     7201 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     7261 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     7321 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     7381 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     7441 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
     7501 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     7561 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     7621 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     7681 gccgcctccc cgcctggaat taattctgca gtcgagacct agaaaaacat ggagcaatca
     7741 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
     7801 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
     7861 ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc
     7921 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     7981 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca
     8041 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct
     8101 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga
     8161 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca
     8221 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc
     8281 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt
     8341 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     8401 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     8461 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     8521 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     8581 aaatgaatat cagagagtga gaggccttga cattgctagc gtttaccgtc gacctctagc
     8641 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     8701 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     8761 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     8821 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     8881 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     8941 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     9001 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     9061 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     9121 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     9181 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     9241 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     9301 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     9361 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     9421 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     9481 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     9541 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     9601 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     9661 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     9721 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     9781 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     9841 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     9901 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     9961 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    10021 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    10081 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
    10141 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    10201 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    10261 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    10321 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    10381 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    10441 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    10501 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    10561 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    10621 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    10681 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    10741 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    10801 aaagtgccac ctgacgtcga cggatcggga gatcaacttg tttattgcag cttataatgg
    10861 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
    10921 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac
    10981 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta
    11041 cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa
    11101 ttgtatttgt taaatatgta ctacaaactt agtagt
//
