LOCUS       sequence-275123-        2790 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FP with ER retention signal for C-term fusion with CDS12 with ER 
            targeting signal peptide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: CTAG_mTurquoise2-HDEL
FEATURES             Location/Qualifiers
     source          1..2790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     117..138
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      213..270
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(294..311)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(465..1053)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(545..564)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1236..1330)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1351..2010)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1925..1944
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2172..2190
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2194..2237)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     CDS             join(2257..2259,2260..2790,1..180)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2299..2320)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2560..2579)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
       61 cactacctga gcacccagtc caagctgagc aaagacccca acgagaagcg cgatcacatg
      121 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
      181 catgatgagc tttaagcttc gagaccctgc agtccggcaa aaaagggcaa ggtgtcacca
      241 ccctgccctt tttctttaaa accgaaaaga ttacttcgcg ttatgcaggc ttcctcgctc
      301 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
      361 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
      421 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca caggctccgc
      481 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
      541 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
      601 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
      661 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
      721 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
      781 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
      841 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
      901 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
      961 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     1021 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     1081 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     1141 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     1201 tatgagtaaa cttggtctga cagctcgagg cttggattct caccaataaa aaacgcccgg
     1261 cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc
     1321 aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact catcgcagta
     1381 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac
     1441 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa
     1501 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac
     1561 ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag
     1621 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc
     1681 gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca
     1741 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac gaaattccgg
     1801 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt
     1861 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca
     1921 ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac
     1981 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
     2041 taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct
     2101 tacgtgcccg atcaactcga gtgccacctg acgtctaaga aaccattatt atcatgacat
     2161 taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa tccttagctt
     2221 tcgctaagga tgatttctgg aattcggtct cattcggtga gcaagggcga ggagctgttc
     2281 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     2341 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     2401 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgtc ctggggcgtg
     2461 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     2521 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     2581 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     2641 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta ctttagcgac
     2701 aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc
     2761 cacaacatcg aggacggcgg cgtgcagctc
//
