LOCUS       sequence-275124-        2778 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FP for C-term fusion with CDS12.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: CTAG_mVenus
FEATURES             Location/Qualifiers
     source          1..2778
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(11..724,725..727))
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(56..77)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     405..424
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     664..685
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     terminator      747..790
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(794..812)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             974..1633
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1040..1059)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1654..1748
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      1931..2519
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2420..2439
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2673..2690
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2714..2771)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
ORIGIN
        1 gtctcgaagc ttacttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga
       61 actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagc ttggactggt
      121 agctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct
      181 ggtagtggtc ggcgagctgc acgccgccgt cctcgatgtt gtggcggatc ttgaagttgg
      241 ccttgatgcc gttcttctgc ttgtcggcgg tgatatagac gttgtggctg ttgtagttgt
      301 actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga
      361 tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt
      421 cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga
      481 agtcgtgctg cttcatgtgg tcggggtagc gggcgaagca ctgcaggccg tagcccaggg
      541 tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatc agcttcaggg
      601 tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt
      661 ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct
      721 tgctcaccga atgagaccga attccagaaa tcatccttag cgaaagctaa ggattttttt
      781 tatctgaaat tctgcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
      841 gtttcttaga cgtcaggtgg cactcgagtt gatcgggcac gtaagaggtt ccaactttca
      901 ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat tttcaggagc
      961 taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg
     1021 gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac
     1081 cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta
     1141 tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat ttcgtatggc
     1201 aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca
     1261 tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt
     1321 tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa
     1381 agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt
     1441 tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata
     1501 ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg
     1561 tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca
     1621 gggcggggcg taatttgata tcgagctcgc ttggactcct gttgatagat ccagtaatga
     1681 cctcagaact ccatctggat ttgttcagaa cgctcggttg ccgccgggcg ttttttattg
     1741 gtgagaatcc aagcctcgag ctgtcagacc aagtttactc atatatactt tagattgatt
     1801 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     1861 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     1921 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     1981 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     2041 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     2101 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     2161 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     2221 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     2281 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     2341 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     2401 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     2461 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctgtggaaaa
     2521 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     2581 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     2641 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcctgca
     2701 taacgcgaag taatcttttc ggttttaaag aaaaagggca gggtggtgac accttgccct
     2761 tttttgccgg actgcagg
//
